Emergence of glycopeptide resistant Enterococcus faecium in Algeria: a case report

Hamidi, Moufida; Ammari, Houria; Ghaffor, M; Benamrouche, Nabila; Tali-Maamar, H.; Tala-Khir, Farida; Younsi, Mokhtar; Rahal, K

doi:10.1684/abc.2012.0781

Cited by 4 publications

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“…This result is of great concern and consistent with other studies, which show a high-level resistance among enterococci, especially in E. faecium , and even more in VREfm. 14 , 25 , 34 The treatment of these enterococcal infections in Algeria is challenging because tigecyclin, daptomycin, linezolid, and quinupristin–dalfopristin are not yet available, and effective therapy for patients infected by MDR VRE is consequently limited, as in our study for patients 2 and 3.…”

Section: Discussionmentioning

confidence: 78%

“…44 However, only two previous reports included the identification of the genetic support for vancomycin resistance, the isolates were VanA-type E. faecium recovered from a blood culture in a 47-year-old severely burned patient in 2010 13 and from a surgical wound in a patient who carried the strain in his digestive tract in 2011, respectively. 14 Our work is therefore the third one in which the vancomycin-resistant genetic support was identified in a VRE. It is also noteworthy that none of the previous study reporting VRE included genotyping.…”

Section: Discussionmentioning

confidence: 83%

“…As in Batna, very few reports have described VRE in other Algerian ( http://www.sante.dz/aarn/alerte.htm ) or North Africa hospitals. 12 , 14 , 40 , 41 In Algeria, the first VRE identified in Algiers University Hospital was an E. faecalis isolate from a urine sample in 2006. 12 More recently, single-center and multicenter epidemiological studies conducted on Enterococcus spp.…”

Section: Discussionmentioning

confidence: 99%

“… 12 However, only few studies identified the genetic support for vancomycin resistance in VRE 13 and there is no previously published molecular epidemiology study of VRE. 14 Here, we report the clinical and bacteriological characteristics of the first cases of HAIs involving VRE in the Batna University Hospital (North-East Algeria) identified during a 6-month period. To our knowledge, here, we performed the third study with the molecular characterization of E. faecium vanA in Algeria and reported the first VRE of sequence type (ST) 789 while performing the first molecular characterization of related cases of HAIs involving VRE in Algeria.…”

Section: Introductionmentioning

confidence: 99%

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First molecular characterization of related cases of healthcare-associated infections involving multidrug-resistant <em>Enterococcus faecium vanA</em> in Algeria

Benammar¹,

Pantel²,

Aujoulat³

et al. 2018

IDR

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PurposeVancomycin-resistant Enterococcus (VRE) faecium (VREfm) are highly resistant bacteria emerging worldwide and rarely studied using molecular tools in Algeria since their first report in 2006. The aim of the study was to investigate healthcare-associated infections (HAIs) involving the first VRE in Batna University Hospital, Algeria, and characterize isolates using molecular tools.Patients and methodsMedical charts were reviewed for patients with VREfm. van genes were detected by multiplex polymerase chain reaction (PCR), and strains were characterized by automated repetitive sequence-based PCR (rep-PCR), multiplex rep-PCR, pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST).ResultsDuring a 6-month period, VREfm infections occurred in four patients hospitalized in three wards. The four isolates were E. faecium vanA belonging to the hospital-adapted clonal complex 17. PCR-based methods did not discriminate the isolates but MLST and PFGE delineated a subgroup of three VREfm of identical pulsotype and sequence type (ST) 80 (yet identified for five isolates in the international PubMLST database) while the fourth isolate was of ST789 (not previously identified for a VREfm) and displayed an unrelated pulsotype. The three genotypically related isolates were recovered in patients who underwent surgery in the same department, suggesting an outbreak for which the source and route of transmission remained unidentified.ConclusionThis first molecular epidemiology study of VRE in Algeria was useful in delimiting an outbreak involving three of the four HAI cases and revealed rarely encountered genotypes. Considering the threat and burden of VRE infections worldwide, particularly in the USA, and the late emergence in Algeria, our study supports the urgent need for improved and early adequate infection control measures to avoid VRE spread in North African hospitals.

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Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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First molecular characterization of related cases of healthcare-associated infections involving multidrug-resistant <em>Enterococcus faecium vanA</em> in Algeria

Benammar¹,

Pantel²,

Aujoulat³

et al. 2018

IDR

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“…In Algeria, the first VRE strain was isolated since 2010. To date, only two papers have reported the emergence of this highly resistant pathogen, one case from Algiers and four cases from Batna [19,20]. To the best of our knowledge, we describe for the first time the microbiological, epidemiological and molecular characteristics of VREfm involved in HAIs from several regions of Algeria.…”

Section: Discussionmentioning

confidence: 92%

Vancomycin-resistant Enterococcus faecium in Algeria: phenotypic and genotypic characterization of clinical isolates

Benamrouche

Guettou

Henniche³

et al. 2021

J Infect Dev Ctries

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Introduction: vancomycin-resistant Enterococcus faecium (VREfm) is a major public health problem worldwide. The aim of our study was to determine the microbiological, epidemiological and molecular characteristics of VREfm isolated in north-central, eastern and western Algeria. Methodology: a collection of 48 VREfm isolated from September 2010 to April 2017 in several Algerian hospitals were studied. Minimum inhibitory concentrations (MICs) were determined by E-test method according to CLSI guidelines. the detection of van genotype of all strains was performed by PCR. Clonal relationship of five VREfm targeted by region were characterized using multilocus sequence typing (MLST). Results: All isolates have multidrug-resistance (MDR) and were resistant to at least five classes of antibiotics; however, all were susceptible to tigecycline and daptomycin with MIC50 at 0.094 µg/mL and 2 µg/mL respectively. All strains belonged to vanA genotype and have high level of resistance to vancomycin and teicoplanin. MLST revealed two sequence types (STs): ST80 (from the four regions of Algeria) and ST789, both belonging to the former hospital-adapted clonal complex CC17. Conclusions: the alarming dissemination of MDR E. faecium vanA and the ST80 in several regions of Algeria suggest a clonal spread of VREfm strains, which urgently require implementation of adequate infection control measures.

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First detection of vanA positive Enterococcus faecium clonal complex 17 in hospital wastewater in Algeria: an epidemiological report

Cherak

Bendjama

Moussi

et al. 2022

New Microbes and New Infections

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Emergence of glycopeptide resistant Enterococcus faecium in Algeria: a case report

Cited by 4 publications

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First molecular characterization of related cases of healthcare-associated infections involving multidrug-resistant <em>Enterococcus faecium vanA</em> in Algeria

First molecular characterization of related cases of healthcare-associated infections involving multidrug-resistant <em>Enterococcus faecium vanA</em> in Algeria

Vancomycin-resistant Enterococcus faecium in Algeria: phenotypic and genotypic characterization of clinical isolates

First detection of vanA positive Enterococcus faecium clonal complex 17 in hospital wastewater in Algeria: an epidemiological report

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