Emergence of gyrovirus 3 in commercial broiler chickens with transmissible viral proventriculitis

Li, Gen; Yuan, Shiyu; He, Min; Zhao, Mingjun; Hao, Xiaoyan; Song, Ming; Zhang, Lingjuan; Qiao, Changming; Huang, Libo; Zhang, Li; Li, Chengui; Wang, Guihua; Cheng, Ziqiang

doi:10.1111/tbed.12927

Cited by 18 publications

(28 citation statements)

References 19 publications

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“…All together raises the question of whether another virus, different from the CPNV, is related to some of these TVP-affected cases. On this line, several other virus including adenovirus (Goodwin, et al, 1996), Gyrovirus (Li et al, 2018) and picornavirus (Kim, et al, 2015), have been reported to be associated with TVP, although none of them could be definitively proved as cause of TVP. The investigation of such potential different aetiologies was however beyond the aims of the current work, and further studies are needed to get further insights in this area.…”

Section: Discussionmentioning

confidence: 99%

Retrospective study on transmissible viral proventriculitis and chicken proventricular necrosis virus (CPNV) in the UK

et al. 2019

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Chicken proventricular necrosis virus (CPNV) is a recently described birnavirus, which has been proposed to be the cause of transmissible viral proventriculitis (TVP). The understanding of the epidemiology of both the virus and the disease is very limited. A retrospective investigation on TVP and CPNV in broiler chicken submissions from the UK from between 1994 and 2015 was performed with the aims of assessing the longitudinal temporal evolution of TVP and CPNV and to review the histological proventricular lesions in the studied chickens. Ninety-nine of the 135 included submissions (73.3%) fulfilled the TVP-diagnostic criteria, while the remaining 36 submissions (26.7%) displayed only lymphocytic proventriculitis (LP). The first detection of CPNV by PCR dated from 2009. Results showed a rise in the number of both TVP and positive CPNV RT-PCR submissions from 2009 with a peak in 2013, suggesting that they may be an emerging or re-emerging disease and pathogen, respectively. Twenty-two out of the 99 submissions displaying TVP lesions (22%) and 4 out of the 36 (11%) ones with LP gave positive CPNV RT-PCR results, further supporting the association between CPNV and TVP and confirming that CPNV is present in a low proportion of proventriculi that do not fulfil the TVP diagnostic criteria. In addition, intranuclear inclusion bodies were observed in 22 of the submissions with TVP. The vast majority of these cases (21 of 22, 96%) gave negative CPNV RT-PCR results, raising the question of whether another virus different from CPNV is responsible for some of these TVPaffected cases. Research highlights • TVP and CPNV are present in the British broilers since at least 1994 and 2009, respectively • TVP and CPNV seem to be an emerging and re-emerging disease and pathogen, respectively • CPNV was detected in proventriculi with both TVP and LP-lesions • Other viruses different from CPNV may be responsible for some TVP-affected cases

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Section: Discussionmentioning

confidence: 99%

Retrospective study on transmissible viral proventriculitis and chicken proventricular necrosis virus (CPNV) in the UK

et al. 2019

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“…The members of the genus Gyrovirus , which consists of the CAV prototype and nine other unofficially recognized species (HGyV/AGV2, GyV3, GyV4, GyV5, GyV6, GyV7, GyV8, GyV9 and GyV10), have been identified in chickens 10,11,17,18 , humans 8,15,16,19,22 , sea birds 21 , mice 25 , ferrets 24,29 , dogs 26 and cats 25 . To date, there is only one report that CAV circulating in domestic cats in China 25 .…”

Section: Discussionmentioning

confidence: 99%

“…HGyV/AGV2 has been identified in sera and tissues of chickens 10–12 , commercial poultry vaccines 13 , the blood of healthy blood donors 14 , HIV-infected patients and organ transplant recipients 15 , and the skin of healthy humans 8 . The third gyrovirus named GyV3 was identified for the first time in the feces of diarrhoeic children from Chile in 2012 16 and had been also found in chickens with transmissible viral proventriculitis and human feces in China 17,18 . GyV4 was found in chicken meat and human stools in Hong Kong 18 .…”

Section: Introductionmentioning

confidence: 99%

Genomic Characterization of Diverse Gyroviruses Identified in the Feces of Domestic Cats

Niu

Dong

et al. 2019

Sci Rep

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Gyroviruses (GyVs) are small, single-stranded, circular DNA viruses in the genus Gyrovirus, which consists of the chicken anemia virus (CAV) prototype and nine other viral species. These different GyV species have been reported in chickens, humans, mice, and companion animals. To date, CAV has been identified in the feces of domestic cats, while the circulation of other GyV species in cats is currently unknown. In the present study, 197 fecal samples were collected from pet cats in northeast China, and samples were screened for different GyV species by PCR. Twelve GyV strains were identified from the feces of pet cats. These included 4 positive for CAV, 3 for HGyV/AGV2, 3 for GyV3 and 2 positive for GyV6. The complete genome sequences of the 12 cat-sourced GyV strains showed 93.9–99.7% nucleotide identities to the homologous reference GyV strains. Phylogenetic analyses based on the complete genomes, VP1, VP2 and VP3 genes showed the identical classification of GyV species with previous reports. Moreover, one and four unique amino acid substitutions were identified in the VP1 protein of the cat-sourced HGyV/AGV2 and GyV6 strains, respectively, and one substitution was also observed in the VP2 protein of one GyV6 strain identified in this study. In conclusion, our investigation demonstrates that the diverse GyV species were circulating in domestic cats, and provides the first molecular evidence for the circulation of HGyV/AGV2, GyV3 and GyV6 in domestic cats. These cat-origin GyVs possessed considerable genetic diversity. This study also raises the possibility that domestic cats, as reservoirs for gyroviruses, may inadvertently disseminate viruses to other species, e.g., humans and chickens.

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“…The PacBio-RSII sequencing platform (Novogene) was used to search proventricular viruses of TVP in chickens. Proventricular samples from diseased chickens were treated as previously described ( 27 ). BLASTx was used to translate sequence subreads and search for similar viral sequences.…”

Section: Methodsmentioning

confidence: 99%

Novel Cyclovirus Identified in Broiler Chickens With Transmissible Viral Proventriculitis in China

Yan

Zhou

et al. 2020

Front. Vet. Sci.

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In October 2018, an outbreak of transmissible viral proventriculitis (TVP) occurred in 30-day-old commercial broiler chickens on a farm in Weifang, China. TVP, an infectious viral disease characterized by runting and stunting, is associated with many viruses, and has a significant economic impact on the global poultry industry. TVP is diagnosed according to clinical symptoms, gross and histological lesions, and negative PCR results for pathogenic bacteria, avian leukosis virus subgroup J, Marek's disease virus, reticuloendotheliosis virus, infectious bursa disease virus, avian reovirus, chicken anemia virus, infectious bronchitis virus, chicken proventricular necrosis virus, gyrovirus 3 and chicken circovirus. To further detect the possible causative pathogens of TVP, we used PacBio third-generation sequencing to examine proventricular samples. A dominant abundance of the novel cyclovirus (CyCV), chCyCV-SDAU-1, was identified in broilers with TVP. The complete chCyCV-SDAU-1 genome was verified via inverse PCR, was 1936 bp long, and consisted of Rep, Cp, and two intergenic regions. Phylogenetic tree analysis showed that chCyCV-SDAU-1 formed an independent branch with other cycloviruses. The homology of chCyCV-SDAU-1 with 20 others known cycloviruses was < 40%. Retrospective investigation showed that the CyCV infection rate in the broilers with TVP was 80% (16/20), while no CyCV was found in healthy chickens. In conclusion, a novel CyCV was identified in chickens with TVP, though its role in this disease is unclear.

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Emergence of gyrovirus 3 in commercial broiler chickens with transmissible viral proventriculitis

Cited by 18 publications

References 19 publications

Retrospective study on transmissible viral proventriculitis and chicken proventricular necrosis virus (CPNV) in the UK

Retrospective study on transmissible viral proventriculitis and chicken proventricular necrosis virus (CPNV) in the UK

Genomic Characterization of Diverse Gyroviruses Identified in the Feces of Domestic Cats

Novel Cyclovirus Identified in Broiler Chickens With Transmissible Viral Proventriculitis in China

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