Emergence of Herpes Simplex Virus-1 Syncytial Variants With Altered Virulence for Mice After Selection With a Natural Carrageenan

Mateu, Cecilia Gabriela; Recalde, Mercedes Pérez; Artuso, María Carolina; Hermida, Gladys N.; Linero, Florencia N.; Scolaro, Luis Alberto; Damonte, Elsa B.; Pujol, Carlos A.; Carlucci, María Josefina

doi:10.1097/olq.0b013e3182084d99

Cited by 12 publications

(15 citation statements)

References 42 publications

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“…Variants of HSV-2 were also obtained under similar selective pressure using nonsulfated compounds. These HCV-2 variants showed resistance to the drug used for selection, as well as to heparin and to the 1C 3 CGN in the order of 2.6 to 6.7 times with respect to the control virus (Mateu et al 2011). However, in these studies, we found that, in vivo, the same HSV-2 variants showed no difference in virulence when compared to the parental strain.…”

Section: Introductionmentioning

confidence: 55%

“…Given that the frequent use of antivirals, such as acyclovir, against HSV may generate resistance, we initiated a series of studies that proposed to examine the behavior of the viral population when subjected to selective pressure using increasing concentrations of natural bioactive compounds during in vitro replication. In earlier studies we found that such selective pressure, employing the 1C3 CGN (precursor of ι and k CGNs), generated variants of HSV-1 with low resistance to CGNs and altered virulence when tested in mice by different routes (Artuso et al 2014;Carlucci et al 2002;Mateu et al 2011). Variants of HSV-2 were also obtained under similar selective pressure using nonsulfated compounds.…”

Section: Introductionmentioning

confidence: 95%

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In vitro isolation of variants of herpes simplex virus attenuated with altered thymidine kinase and DNA polymerase genes using carrageenans as selection agents

et al. 2016

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Natural polysaccharides known as carrageenans are potent and selective inhibitors of herpes simplex virus through blocking the interaction of the virus with the cellular receptor. Passaging the virus in vitro in the presence of carrageenans types k and ι enabled us to generate variants of herpes simplex type 2. At passage 22, four clones were selected for further characterization: ι22-9, ι22-12, k22-12 and k22-13. Variants showed a syncytial phenotype, grew at similar titers when compared to parental virus and exhibited moderate resistance to carrageenans. These were found to have a mutation in the thymidine kinase gene in the case of k22-13 (aa 149 Val to Ala) and in the DNA pol gene in the case of ι22-12 (aa 789 Met to Thr). In variant k22-12, three substitutions in the DNA pol gene were identified. Variants were less virulent than parental strain when tested intravaginally or intranasally in mice. Attenuation correlated with higher levels of IL-6 and TNF-α in animals inoculated with the variants. Selective pressure on the external glycoproteins of the virus may generate viruses with alterations in genes unrelated to the target of action of the selection agent. Study of this type of variation might help us to understand the basis of persistent viral strategies, which in turn may play a role in the development of virus-host symbioses.

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Section: Introductionmentioning

confidence: 55%

Section: Introductionmentioning

confidence: 95%

In vitro isolation of variants of herpes simplex virus attenuated with altered thymidine kinase and DNA polymerase genes using carrageenans as selection agents

et al. 2016

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“…In experiments performed on the Balb/c mice, intravag inal infection with viral clones passaged in the pres ence of natural carrageenan from the red seaweed Gigartina scottbergii, did not cause disease in animals, while the untreated original strain was fatal to them [78]. Attenuation of the herpesvirus strains correlated with low levels of TNFα, IL 6, and IFNγ in the vagi nal content.…”

Section: Action Of Algal Sulfated Polysaccharides On Genital Herpesvimentioning

confidence: 96%

“…Mateu et al showed that carrageenans can reduce the virulence of the Herpes simplex virus [78]. In experiments performed on the Balb/c mice, intravag inal infection with viral clones passaged in the pres ence of natural carrageenan from the red seaweed Gigartina scottbergii, did not cause disease in animals, while the untreated original strain was fatal to them [78].…”

Section: Action Of Algal Sulfated Polysaccharides On Genital Herpesvimentioning

confidence: 99%

Antiviral activity and pathogenetic targets for seaweed sulfated polysaccharides in herpesvirus infections

Беседнова¹,

Макаренкова²,

Zvyagintseva

et al. 2016

Biochem. Moscow Suppl. Ser. B

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The review summarizes results of studies of effects of sulfated polysaccharides from seaweed on herpesviruses and the course of herpesvirus infections. Importance of this problem is determined by the prev alence of herpesviruses that can persist in the human body and demonstrate a high degree of immune mimicry and resistance to antiviral drugs. A wide range of physiological action of sulfated polysaccharides, receptor agonists of innate and adaptive immune cells, which possess potent antiviral, antioxidant and anti inflamma tory activities, open the possibility of their use for creation of new generation pharmacological substances and drugs with associated activity for the treatment of herpesvirus infections.

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“…After the viral selection and cloning, all the variants showed a syncytial phenotype on Vero cells, this phenotype was also observed on mouse lung and genitals primary cell cultures [74,75]. In order to characterize the obtained viral variants, the susceptibility to CGNs was assessed.…”

Section: Effect Of Carrageenans On the Virusmentioning

confidence: 97%

Polysaccharides from Red Algae: Genesis of a Renaissance

Carlucci¹,

Mateu²,

Artuso³

et al. 2012

The Complex World of Polysaccharides

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Emergence of Herpes Simplex Virus-1 Syncytial Variants With Altered Virulence for Mice After Selection With a Natural Carrageenan

Cited by 12 publications

References 42 publications

In vitro isolation of variants of herpes simplex virus attenuated with altered thymidine kinase and DNA polymerase genes using carrageenans as selection agents

In vitro isolation of variants of herpes simplex virus attenuated with altered thymidine kinase and DNA polymerase genes using carrageenans as selection agents

Antiviral activity and pathogenetic targets for seaweed sulfated polysaccharides in herpesvirus infections

Polysaccharides from Red Algae: Genesis of a Renaissance

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