Emergence of human-porcine reassortment G9P[19] porcine rotavirus A strain in Guangdong Province, China

Luo, Shicheng; Chen, Xiuqiao; Yan, Guangzhi; Chen, Shengnan; Pan, Jinghua; Zeng, Mengyi; Han, Hui; Guo, Yajing; Zhang, Haoquan; Li, Jiaming; Mo, Meilian; Liu, Mingjie; Huang, Li

doi:10.3389/fvets.2022.1111919

Cited by 9 publications

(7 citation statements)

References 26 publications

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“…It cannot be ignored that, as a major veterinary pathogen, RV significantly affects agricultural production, mainly involving weaning and post-weaning piglets, young calves, young sheep/goats as well as foals [ 31 ]. In a pig farm from the Guangdong Province of China, according to one previous study, the morbidity and mortality of RV were 60.00% and 20.99% in 2022, respectively [ 7 ].…”

Section: Discussionmentioning

confidence: 99%

“…Several studies on the reassortment or inter-species transmission of human and porcine RV strains are published annually. Examples include the GDJM1 strain (PoRVA) from China in 2022 [ 7 ], the CN127 strain (PoRVA) from China in 2020 [ 8 ], the SO1199 strain (HuRVA) from Japan in 2020 [ 9 ], and the KCH148 strain (HuRVA) from Kenya in 2019 [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

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Emergence of a Novel G4P[6] Porcine Rotavirus with Unique Sequence Duplication in NSP5 Gene in China

Zhou,

Hou,

Xiao

et al. 2024

Animals

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Rotavirus is a major causative agent of diarrhoea in children, infants, and young animals around the world. The associated zoonotic risk necessitates the serious consideration of the complete genetic information of rotavirus. A segmented genome makes rotavirus prone to rearrangement and the formation of a new viral strain. Monitoring the molecular epidemiology of rotavirus is essential for its prevention and control. The quantitative RT-PCR targeting the NSP5 gene was used to detect rotavirus group A (RVA) in pig faecal samples, and two pairs of universal primers and protocols were used for amplifying the G and P genotype. The genotyping and phylogenetic analysis of 11 genes were performed by RT-PCR and a basic bioinformatics method. A unique G4P[6] rotavirus strain, designated S2CF (RVA/Pig-tc/CHN/S2CF/2023/G4P[6]), was identified in one faecal sample from a piglet with severe diarrhoea in Guangdong, China. Whole genome sequencing and analysis suggested that the 11 segments of the S2CF strain showed a unique Wa-like genotype constellation and a typical porcine RVA genomic configuration of G4-P[6]-I1-R1-C1-M1-A8-N1-T1-E1-H1. Notably, 4 of the 11 gene segments (VP4, VP6, VP2, and NSP5) clustered consistently with human-like RVAs, suggesting independent human-to-porcine interspecies transmission. Moreover, a unique 344-nt duplicated sequence was identified for the first time in the untranslated region of NSP5. This study further reveals the genetic diversity and potential inter-species transmission of porcine rotavirus.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Emergence of a Novel G4P[6] Porcine Rotavirus with Unique Sequence Duplication in NSP5 Gene in China

Zhou,

Hou,

Xiao

et al. 2024

Animals

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“…Currently, about 42 G genotypes and 58 P genotypes have been reported to the RVA Classification Working Group (RCWG) (https://rega.kuleuven.be/cev/viralmetagenomics/virus-classification/rcwg). 7 Among these, several genotype combinations, including G1P[8], G2P[4], G3P[8], G4P[8], G9P[8] and G12P[8], together account for an estimated 90‐95% of all RVA infections worldwide 4,8 . Interestingly, G8P[8] has been detected in China and elsewhere outside Africa and becomes predominant in recent years, apparently on post‐COVID‐19 pandemic period 9–11 …”

Section: Introductionmentioning

confidence: 99%

“…4 Currently, about 42 G genotypes and 58 P genotypes have been reported to the RVA Classification Working Group (RCWG) (https://rega.kuleuven.be/ cev/viralmetagenomics/virus-classification/rcwg). 7 Among these, several genotype combinations, including G1P [8], G2P [4], G3P [8],…”

mentioning

confidence: 99%

Emergence and high prevalence of unusual rotavirus G8P[8] strains in outpatients with acute gastroenteritis in Shanghai, China

Zhong,

Jia,

et al. 2024

Journal of Medical Virology

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Group A rotavirus (RVA) is considered an important cause of acute gastroenteritis (AGE) in all age groups, especially in children. We investigated the epidemiology of RVA in outpatients aged ≤ 16 years at the Children's Hospital of Fudan University, Shanghai, China. In this study, 16.6% (246/1482) were infected with RVA. The detection rate of RVA was significantly higher in the year of 2021 (20.3%, 147/725) compared to the year of 2020 (14.5%, 77/531) and 2022 (9.7%, 22/226) (p = 0.000). RVA infection was prevalent in all seasons from 2020 to 2022, with a different monthly distribution observed in different years. Among 246 RVA‐positive samples, 14 different RVA genotypes were detected with different frequencies. Overall, G9P[8] (45.5%, 112/246) was the most common RVA genotype, followed by G8P[8] (37.4%, 92/246) and G3P[8] (4.1%, 10/246). The prevalence of G/P combinations varied from 2020 to 2022. G9P[8] was the most prevalent circulating genotype in 2020 (68.2%, 15/22) and 2021 (57.8%, 85/147). However, G8P[8] (68.8%, 53/77) suddenly became the most prevalent genotype in 2022 after being first identified in 2020 and prevalent in 2021. The G8 strains detected in the study were all clustered to DS‐1‐like G8 strains with the closest genetic distance to strains circulating in Southeast Asia. Our study demonstrated the diversity of circulating RVA genotypes in Shanghai. The sudden emergence and high prevalence of unusual G8P[8] strains deserve more concern and indicate the need for continuous surveillance of RVA in children with AGE in the future to refine future vaccine strategy.

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“…Another porcine enteric virus, PoRVA, is a non-enveloped double-stranded RNA virus belonging to the genus Rotavirus and family Reoviridae. It is one of the major pathogens responsible for severe diarrhea in piglets worldwide (Vlasova et al, 2017;Luo et al, 2023). The PoRVA genome is approximately 18.5 kb and has 11 double-stranded RNA segments.…”

Section: Introductionmentioning

confidence: 99%

Development of a triplex quantitative reverse transcription-polymerase chain reaction for the detection of porcine epidemic diarrhea virus, porcine transmissible gastroenteritis virus, and porcine rotavirus A

Luo,

Li,

et al. 2024

Front. Microbiol.

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Porcine viral diarrhea is caused by many pathogens and can result in watery diarrhea, dehydration and death. Various detection methods, such as polymerase chain reaction (PCR) and real-time quantitative PCR (qPCR), have been widely used for molecular diagnosis. We developed a triplex real-time quantitative reverse transcription PCR (qRT-PCR) for the simultaneous detection of three RNA viruses potentially associated with porcine viral diarrhea: porcine epidemic diarrhea virus (PEDV), porcine transmissible gastroenteritis virus (TGEV), and porcine rotavirus A (PoRVA). The triplex qRT-PCR had R2 values of 0.999 for the standard curves of PEDV, TGEV and PoRVA. Importantly, the limits of detection for PEDV, TGEV and PoRVA were 10 copies/μL. The specificity test showed that the triplex qRT-PCR detected these three pathogens specifically, without cross-reaction with other pathogens. In addition, the approach had good repeatability and reproducibility, with intra-and inter-assay coefficients of variation <1%. Finally, this approach was evaluated for its practicality in the field using 256 anal swab samples. The positive rates of PEDV, TGEV and PoRVA were 2.73% (7/256), 3.91% (10/256) and 19.14% (49/256), respectively. The co-infection rate of two or more pathogens was 2.73% (7/256). The new triplex qRT-PCR was compared with the triplex RT-PCR recommended by the Chinese national standard (GB/T 36871-2018) and showed 100% agreement for PEDV and TGEV and 95.70% for PoRVA. Therefore, the triplex qRT-PCR provided an accurate and sensitive method for identifying three potential RNA viruses for porcine viral diarrhea that could be applied to diagnosis, surveillance and epidemiological investigation.

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Emergence of human-porcine reassortment G9P[19] porcine rotavirus A strain in Guangdong Province, China

Cited by 9 publications

References 26 publications

Emergence of a Novel G4P[6] Porcine Rotavirus with Unique Sequence Duplication in NSP5 Gene in China

Emergence of a Novel G4P[6] Porcine Rotavirus with Unique Sequence Duplication in NSP5 Gene in China

Emergence and high prevalence of unusual rotavirus G8P[8] strains in outpatients with acute gastroenteritis in Shanghai, China

Development of a triplex quantitative reverse transcription-polymerase chain reaction for the detection of porcine epidemic diarrhea virus, porcine transmissible gastroenteritis virus, and porcine rotavirus A

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