Emergence of
 IntI1
 associated
 bla
 VIM-2
 gene cassette-mediated carbapenem resistance in opportunistic pathogen
 Pseudomonas stutzeri

Bashar, Sabrin; Sanyal, Santonu Kumar; Sultana, Munawar; Hossain, M. Anwar

doi:10.1038/emi.2017.12

Cited by 5 publications

(4 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar resistance modules may be found either in different plasmids or in the chromosome, evidencing mobilization of such modules. 1040 Also, a given bla MBL gene may be inserted in different immediate contexts, 1041 especially when mobilized as gene cassettes, or exist in multiple copies in the same host. 1042 Unfortunately, many reports of bla MBL genes lack the characterization of the DNA sequences extending beyond the immediate elemental modules.…”

Section: Horizontal Gene Transfer Of Bla Mbl Genesmentioning

confidence: 99%

Metallo-β-lactamases in the Age of Multidrug Resistance: From Structure and Mechanism to Evolution, Dissemination, and Inhibitor Design

2021

View full text Add to dashboard Cite

Antimicrobial resistance is one of the major problems in current practical medicine. The spread of genes coding for resistance determinants among bacteria challenges the use of approved antibiotics, narrowing the options for treatment. Resistance to carbapenems, last resort antibiotics, is a major concern. Metallo-β-lactamases (MBLs) hydrolyze carbapenems, penicillins, and cephalosporins, becoming central to this problem. These enzymes diverge with respect to serine-β-lactamases by exhibiting a different fold, active site, and catalytic features. Elucidating their catalytic mechanism has been a big challenge in the field that has limited the development of useful inhibitors. This review covers exhaustively the details of the active-site chemistries, the diversity of MBL alleles, the catalytic mechanism against different substrates, and how this information has helped developing inhibitors. We also discuss here different aspects critical to understand the success of MBLs in conferring resistance: the molecular determinants of their dissemination, their cell physiology, from the biogenesis to the processing involved in the transit to the periplasm, and the uptake of the Zn(II) ions upon metal starvation conditions, such as those encountered during an infection. In this regard, the chemical, biochemical and microbiological aspects provide an integrative view of the current knowledge of MBLs.

show abstract

Section: Horizontal Gene Transfer Of Bla Mbl Genesmentioning

confidence: 99%

Metallo-β-lactamases in the Age of Multidrug Resistance: From Structure and Mechanism to Evolution, Dissemination, and Inhibitor Design

2021

View full text Add to dashboard Cite

show abstract

“…It is reported that 62% of all globally reported cases were detected in the Mediterranean Basin. 12 However, no strains from the Mediterranean region are found in Figure 3 . Two different explanations are proposed for this phenomenon.…”

Section: Discussionmentioning

confidence: 98%

“…11 In 2017, class I integron containing bla VIM-2 emerged in P. stutzeri in Bangladesh. 12 However, the carbapenemase resistance genes detected in P. stutzeri (except bla DIM-1 ) were identified as chromosomal located. The discovery of transferable plasmid carrying bla VIM-2 gene in P. stutzeri has not so far been reported.…”

Section: Introductionmentioning

confidence: 98%

Emergence and Genetic Characterization of Plasmid-Encoded VIM-2-Producing Pseudomonas stutzeri with Novel Integron In1998 Isolated from Cerebrospinal Fluid

Liu¹,

Xu²,

Guo³

et al. 2021

IDR

View full text Add to dashboard Cite

Purpose: To investigate the genomic and plasmid characteristics of a newly discovered Pseudomonas stutzeri strain with a bla VIM-2 -carrying plasmid and novel integron In1998 isolated from a cerebrospinal fluid specimen in a teaching hospital. Methods: Species identification was performed by MALDI-TOF MS, and bla VIM-2 was identified by PCR and Sanger sequencing. Whole-genome sequencing analysis was conducted using the Illumina NovaSeq 6000 and Oxford Nanopore platforms. Integron detection was performed using INTEGRALL. The phylogenetic tree was constructed by using kSNP3.0. Plasmid characteristics were assessed by S1-pulsed-field gel electrophoresis (S1-PFGE), Southern blotting, conjugation experiments, and whole-genome sequencing analysis. Comparative genomics analysis of the plasmid and genetic context of bla VIM-2 were conducted by using BLAST Ring Image Generator (BRIG) and Easyfig 2.3, respectively. Results: ZDHY95, an MDR strain of P. stutzeri harboring bla VIM-2 , was identified. It was sensitive only to amikacin and was resistant to carbapenems, β-lactams, aztreonam, fluoroquinolones, and aminoglycosides. Joint S1-PFGE, Southern blot, conjugation assay, and whole-genome sequencing experiments confirmed that the bla VIM-2 gene was located within class I integron In1722 of the plasmid and that the surrounding genetic environment was 5ʹCS-aacA4ʹ-30-bla VIM-2 -aacA4ʹ-3ʹCS. The novel class I integron In1998 was detected on the chromosome of P. stutzeri ZDHY95, and the gene cassette array was 5ʹCS-aacA3-aadA13-cmlA8-bla OXA-246 -arr3-dfrA27 -3ʹCS. Phylogenetic analysis showed that antimicrobial resistance gene-carrying P. stutzeri isolates were divided into two clusters, mainly containing isolates from the USA and Pakistan. Conclusion:A novel bla VIM-2 -carrying conjugative plasmid, pZDHY95-VIM-2, was reported for the first time in P. stutzeri, elucidating the genetic environment and transfer mechanism. The gene structure of the novel class I integron In1998 was also clarified. We explored the phylogenetic relationship of P. stutzeri with drug resistance genes and suggested that Pseudomonas with metallo-β-lactamases (MBLs) in the hospital environment may cause infection in patients with long-term intubation or after interventional surgery.

show abstract

“…Pseudomonas stutzeri is a non-fluorescent denitrifying bacterium widely distributed in the environment [27]. Studies have shown that Pseudomonas stutzeri plays an important role in nitrogen fixation, denitrification, organic sulfur degradation, and degradation of aromatic compounds [28][29][30][31]. In the early stage of the experiment, the research team analyzed the whole genome sequence of Pseudomonas stutzeri LH-42 and found nineteen genes for the two-component system from the whole genome of Pseudomonas stutzeri LH-42.…”

Section: Plos Onementioning

confidence: 99%

The deletion of HK-1 gene affects the bacterial virulence of Pseudomonas stutzeri LH-42

Shan

2022

PLoS ONE

View full text Add to dashboard Cite

Two-component systems (TCSs) are widespread regulatory systems in bacteria, which control cellular functions and play an important role in sensing various external stimuli and regulating gene expression in response to environmental changes. Among the nineteen genes for the two-component system found in the whole genome of Pseudomonas stutzeri LH-42, one of the TCS coded by the HK-1 gene, has a structural domain similar to the HAMP domain, which plays an important role in regulating bacterial virulence in other bacteria. In this study, the deletion mutant LH-42△HK-1 was successfully constructed using the lambda Red recombinase system. Compared with the wild-type strain, the mutant strain LH-42△HK-1 showed a significantly slower growth time and a longer stationary phase time. In addition, in the plate bacteriostatic experiment with Escherichia coli DH5α as an indicator strain, the inhibition zone size of the mutant strain showed significantly less than the wild-type strain(P<0.05), indicating that the virulence of the mutant strain was significantly reduced compared with the wild-type strain. Overall, the results indicate that the deletion of the gene HK-1 decreased bacterial virulence in Pseudomonas stutzeri LH-42.

show abstract

Emergence of IntI1 associated bla _VIM-2 gene cassette-mediated carbapenem resistance in opportunistic pathogen Pseudomonas stutzeri

Cited by 5 publications

References 16 publications

Metallo-β-lactamases in the Age of Multidrug Resistance: From Structure and Mechanism to Evolution, Dissemination, and Inhibitor Design

Metallo-β-lactamases in the Age of Multidrug Resistance: From Structure and Mechanism to Evolution, Dissemination, and Inhibitor Design

Emergence and Genetic Characterization of Plasmid-Encoded VIM-2-Producing Pseudomonas stutzeri with Novel Integron In1998 Isolated from Cerebrospinal Fluid

The deletion of HK-1 gene affects the bacterial virulence of Pseudomonas stutzeri LH-42

Contact Info

Product

Resources

About

Emergence of IntI1 associated bla VIM-2 gene cassette-mediated carbapenem resistance in opportunistic pathogen Pseudomonas stutzeri

Cited by 5 publications

References 16 publications

Metallo-β-lactamases in the Age of Multidrug Resistance: From Structure and Mechanism to Evolution, Dissemination, and Inhibitor Design

Metallo-β-lactamases in the Age of Multidrug Resistance: From Structure and Mechanism to Evolution, Dissemination, and Inhibitor Design

Emergence and Genetic Characterization of Plasmid-Encoded VIM-2-Producing Pseudomonas stutzeri with Novel Integron In1998 Isolated from Cerebrospinal Fluid

The deletion of HK-1 gene affects the bacterial virulence of Pseudomonas stutzeri LH-42

Contact Info

Product

Resources

About

Emergence of IntI1 associated bla _VIM-2 gene cassette-mediated carbapenem resistance in opportunistic pathogen Pseudomonas stutzeri