Emergence of Klebsiella pneumoniae and Salmonella Metallo-Beta-Lactamase (NDM-1) Producers on Reunion Island

Cabanes, Florian; Lemant, Jérôme; Picot, S.; Simac, C.; Cousty, Julien; Jalin, Laurence; Naze, Florence; Boisson, Véronique; Cresta, Marie-Pierre; Andre, Hélène; Thibault, Laure; Tixier, François; Winer, Arnaud; Antok, Emmanuel; Michault, Alain

doi:10.1128/jcm.01029-12

Cited by 29 publications

(23 citation statements)

References 3 publications

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“…Our results may partially explain the low frequency of VIM-2-producing salmonellae; however, recent reports of Salmonella carrying bla NDM (9,10,11) suggest that the latter might impose a lower biological cost on its host. Further studies are required in order to confirm or rule out such a hypothesis.…”

Section: Discussioncontrasting

confidence: 40%

“…Furthermore, only a few reports concerning the occurrence of MBL in clinical isolates of this genus have been published; although epidemiologically unrelated, such isolates carried all bla NDM-1 (9,10,11), and recently, VIM-2. Consequently, the extremely low frequency of class B ␤-lactamase-producing salmonellae, together with the data presented in this work, support the hypothesis that the expression of such enzymes in this particular microorganism might be accompanied by an important fitness reduction.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, the occurrence of metallo-␤-lactamase (MBL)-producing salmonellae is an infrequent event. In recent years, a few clinical cases have been reported, all of them corresponding to NDM-producing Salmonella (10,11,12). Interestingly, VIM alleles were not reported in Salmonella isolates until 2013 (9,13).…”

mentioning

confidence: 99%

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Synthesis of Metallo-β-Lactamase VIM-2 Is Associated with a Fitness Reduction in Salmonella enterica Serovar Typhimurium

Cordeiro

Chabalgoity

Yim

et al. 2014

Antimicrob Agents Chemother

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Antibiotic resistance, especially due to ␤-lactamases, has become one of the main obstacles in the correct treatment of Salmonella infections; furthermore, antibiotic resistance determines a gain of function that may encompass a biological cost, or fitness reduction, to the resistant bacteria. The aim of this work was to determine in vitro if the production of the class B ␤-lactamase VIM-2 determined a fitness cost for Salmonella enterica serovar Typhimurium. To that end the gene bla VIM-2 was cloned into the virulent strain S. Typhimurium SL1344, using both the tightly regulated pBAD22 vector and the natural plasmid pST12, for inducible and constitutive expression, respectively. Fitness studies were performed by means of motility, growth rate, invasiveness in epithelial cells, and plasmid stability. The expression of bla VIM-2 was accompanied by alterations in micro-and macroscopic morphology and reduced growth rate and motility, as well as diminished invasiveness in epithelial cells. These results suggest that VIM-2 production entails a substantial fitness cost for S. Typhimurium, which in turn may account for the extremely low number of reports of metallo-␤-lactamase-producing Salmonella spp.

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Section: Discussioncontrasting

confidence: 40%

Section: Discussionmentioning

confidence: 99%

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Synthesis of Metallo-β-Lactamase VIM-2 Is Associated with a Fitness Reduction in Salmonella enterica Serovar Typhimurium

Cordeiro

Chabalgoity

Yim

et al. 2014

Antimicrob Agents Chemother

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“…Three months later, a patient transferred from a hospital in India to the same unit in Reunion Island yielded growth of NDM-1-producing Salmonella enterica subsp. enterica serotype Westhampton from a urine culture (Cabanes et al, 2012).…”

Section: A P Johnson and N Woodfordmentioning

confidence: 99%

Global spread of antibiotic resistance: the example of New Delhi metallo-β-lactamase (NDM)-mediated carbapenem resistance

Johnson¹,

Woodford²

2013

Journal of Medical Microbiology

382

286

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The rapidity with which new types of antibiotic resistance can disseminate globally following their initial emergence or recognition is exemplified by the novel carbapenemase New Delhi metallo-blactamase (NDM). The first documented case of infection caused by bacteria producing NDM occurred in 2008, although retrospective analyses of stored cultures have identified the gene encoding this enzyme (bla NDM ) in Enterobacteriaceae isolated in 2006. Since its first description, NDM carbapenemase has been reported from 40 countries worldwide, encompassing all continents except South America and Antarctica. The spread of NDM has a complex epidemiology involving the spread of a variety of species of NDM-positive bacteria and the interstrain, inter-species and inter-genus transmission of diverse plasmids containing bla NDM , with the latter mechanism having played a more prominent role to date. The spread of NDM illustrates that antibiotic resistance is a public health problem that transcends national borders and will require international cooperation between health authorities if it is to be controlled.

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“…E. coli and Klebsiella isolates showing an ESBL phenotype, but no imipenem resistance were obtained from the same patient. No data on the presence of bla NDM-1 in these isolates were presented (Cabanes et al, 2012).…”

Section: Carbapenemase-producing Salmonella Spp Isolates From Cases mentioning

confidence: 99%

Scientific Opinion on Carbapenem resistance in food animal ecosystems

Publication¹

2013

EFS2

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Carbapenems are broad-spectrum β-lactam antimicrobials used for the treatment of serious infections in humans. To date only sporadic studies have reported the occurrence of carbapenemase-producing (CP) bacteria in foodproducing animals and their environment. The bacteria and enzymes isolated include VIM-1 producing Escherichia coli and Salmonella Infantis from pigs and poultry in Germany, OXA-23-producing Acinetobacter spp. from cattle and horses in France and Belgium, and NDM-producing Acinetobacter spp. from pigs and poultry in China. In the German S. Infantis and E. coli isolates, the VIM-1-encoding genes were located on IncHI2 plasmids. A methodology including selective culture is proposed for the detection of CP strains of Enterobacteriaceae and Acinetobacter spp. The choice of selective media for the surveillance of carbapenem resistance for testing animal and food samples needs to be experimentally evaluated and validated. Biochemical and phenotypic tests for the confirmatory identification of CP bacteria are available. For CP bacteria in animals and food, active/passive monitoring and/or targeted surveys should cover key zoonotic agents, animal pathogens and indicator organisms. Priority should be given to broilers, fattening turkeys, fattening pigs, veal calves and meat thereof. Because there are no data on the comparative efficacy of individual control options, prioritisation is complex. Continued prohibition of the use of carbapenems in food-producing animals would be a simple and effective option. As genes encoding carbapenemase production are mostly plasmid-mediated, and co-resistance may be an important issue in the spread of such resistance mechanisms, decreasing the frequency of use of antimicrobials in animal production in the EU in accordance with prudent use guidelines is also of high priority. The effectiveness of any control measures should be monitored by targeted surveys, using selective isolation methods and pre-enrichment of samples. Control measures should be proactively implemented at national and international levels to prevent CP strains become widespread in livestock. SUMMARYThe European Food Safety Authority (EFSA) asked the Panel on Biological Hazards (BIOHAZ) to provide a scientific opinion on the carbapenem resistance in food animal ecosystems. Carbapenems are broad-spectrum beta (β)-lactam antimicrobials mostly used for the treatment of serious infections in humans, frequently in hospitalised patients, and are considered last-line therapy for infections caused by multidrug-resistant Gram-negative bacteria. In particular, the panel was asked: (i) to define the carbapenemase-producing (CP) bacterial strains and genes relevant for public health and linked to food-producing animals or food-borne transmission; (ii) to review the information on the epidemiology of acquired resistance to carbapenems, including the genes coding for such resistance, in food-producing animals and food; (iii) to perform a critical analysis of the methods (phenotypic and genotypic) and the interpr...

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Emergence of Klebsiella pneumoniae and Salmonella Metallo-Beta-Lactamase (NDM-1) Producers on Reunion Island

Cited by 29 publications

References 3 publications

Synthesis of Metallo-β-Lactamase VIM-2 Is Associated with a Fitness Reduction in Salmonella enterica Serovar Typhimurium

Synthesis of Metallo-β-Lactamase VIM-2 Is Associated with a Fitness Reduction in Salmonella enterica Serovar Typhimurium

Global spread of antibiotic resistance: the example of New Delhi metallo-β-lactamase (NDM)-mediated carbapenem resistance

Scientific Opinion on Carbapenem resistance in food animal ecosystems

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