Emergence of mcr-1 gene and carbapenemase-encoding genes among colistin-resistant Klebsiella pneumoniae clinical isolates in Jordan

Gharaibeh, Mohammad; Alyafawi, Dania A.; Elnasser, Ziad; Lafi, Shawkat Q.; Obeidat, Haneen

doi:10.1016/j.jiph.2022.07.005

Cited by 16 publications

(11 citation statements)

References 29 publications

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“…Our results showed that the blaKPC and blaOXA-48 were more predominant than blaNDM-1, blaIMP and blaVIM among colistin sensitive and colistin resistant (Col R) CRKp. This goes around with Gharaibeh et al 30 who found that blaKPC was the most frequently found in either colistin sensitive (6.6%) or colistin resistant Kp (6.7%). followed by blaNDM among colistin sensitive Kp (3.3%) and blaIMP among Col R Kp, also matched with other studies 31,32 .…”

Section: Table (5): Antibiotic Susceptibility Of Cpkp In Relation Tomentioning

confidence: 72%

“…Also, studies on CRE in Hong Kong 33 and Oman 34 revealed presence of mcr1 in only 6.5% and 4.5% respectively. This can be explained by that, the most common cause of colistin resistance in Klebsiella pneumoniae isolates is chromosomal mutation that modifies LPS resulting in decreasing their negative charges and so decrease their affinity to colistin 30 .…”

Section: Table (5): Antibiotic Susceptibility Of Cpkp In Relation Tomentioning

confidence: 99%

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Colistin Susceptibility among Carbapenem Resistant Klebsiella pneumoniae Isolated from Menoufia University Hospitals

Elaskary¹,

Badawy²

2023

The Egyptian Journal of Hospital Medicine

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Background: Infections by CRKp represents a major health problem. Carbapenemases have been frequently reported in CRKp. Limited antimicrobials are still effective for CRKp like tigecycline, ceftazidime/avibactam, as well, polymyxins including colistin. With the increased use of colistin, Col R k. pneumoniae carbapenemase (KpC)producing isolates are documented worldwide. Objectives: The aim of the current study is to investigate carbapenemases and to determine colistin susceptibility and mcr genes among CRKp. Material and methods: k. pneumoniae were identified by conventional methods and API20E. Carbapenem and colistin resistance were screened by disc diffusion method. Confirmatory combined mCIM/eCIM testing was used for carbapenemases detection. BMD and CBDE tests were used for colistin susceptibility detection. Multiplex PCRs were done for carbapenemases genes (blaNDM-1, blaOXA48, blaIMP blaVIM, blaKPC) and colistin resistance genes (mcr1, 2, 3, 4 and 5 genes) detection. Results: Out of CRKp, 68.9% were positive carbapenemases by mCIM/eCIM testing, 33.3% of them were MBLs and 66.7% were serine carbapenemases. the most frequent carbapenemases genes were blaKPC and blaOXA-48. About 16.3% of CRKp were colistin resistant by BMD test. There was fair agreement of CBDE test in relation to BMD for colistin susceptibility detection. The mcr1 gene was detected in 9.1% of CRKp. Conclusions: The increase detection of carbapenemases and colistin resistance among k. pneumoniae isolates obligate us for efficient use of prevention and control protocols to decrease the multidrug resistance in hospital and community environments.

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Section: Table (5): Antibiotic Susceptibility Of Cpkp In Relation Tomentioning

confidence: 72%

Section: Table (5): Antibiotic Susceptibility Of Cpkp In Relation Tomentioning

confidence: 99%

Colistin Susceptibility among Carbapenem Resistant Klebsiella pneumoniae Isolated from Menoufia University Hospitals

Elaskary¹,

Badawy²

2023

The Egyptian Journal of Hospital Medicine

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“…Based on MIC tests, 41 isolates (46%) were phenotypically resistant to colistin but contained no mobile colistin resistance genes (mcr1-5, and 9). Indeed, several recent Jordanian studies have investigated the presence of the mcr gene in various types of bacteria found in animals and humans ( Gharaibeh et al, 2022 , Gharaibeh et al, 2024a , Gharaibeh et al, 2024b , Gharaibeh et al, 2024c ). The present study is the first to examine the presence of mcr genes in Salmonella isolated from poultry in Jordan.…”

Section: Discussionmentioning

confidence: 99%

Occurrence, virulence, and resistance genes in Salmonella enterica isolated from an integrated poultry company in Jordan

Gharaibeh,

Lafi,

Allah

et al. 2024

Poultry Science

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“…In recent years A. baumannii research in Jordan has focused on its phenotypic and genotypic resistance profiles, the mechanism of drug resistance and clinical epidemiology with little attention on the mechanism of virulence and pathogenesis [25,32,33]. In this research, molecular and conventional assays were conducted to inspect the virulence genes harbored in CRAB strains isolated from clinical samples in two hospitals in Jordan.…”

Section: Discussionmentioning

confidence: 99%

Virulence Molecular Epidemiology of Clinical Carbapenem-Resistant Acinetobacter baumannii: First Report from Jordan

Zueter,

Al Balawi,

Al-Tamimi

et al. 2024

Preprint

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1) Background: carbapenem-resistant Acinetobacter baumannii (CRAB) is an opportunistic Gram-negative pathogen that has a significant role in healthcare-associated infections. Unlike several studies on the antibiotic-resistant epidemiology of A. baumanni, virulence molecular epidemiology was less studied. This study aimed to investigate CRAB virulence genes and their ability to form biofilms, and to correlate their biofilm formation ability with both; biofilm-encoding virulence genes and carbapenemase-encoding resistance genes. 2) Methods: 107 CRAB clinical isolates were collected from two hospitals in Jordan between 2018 and 2019 and were screened for virulence genes using PCR. In addition, biofilm formation ability was assessed using the microtiter plate method. 3) Results: the frequencies of the bap, OmpA, surA, PLD, paaE, basD, and traT virulence genes were 99.10%, 98.20%, 98.20%, 95.50%, 89.10%, 86.40%, and 8.20%, respectively. Overall, 86.4% of the tested isolates were biofilm formers with varying degrees; weak (28.2%), moderate (36.4%) and strong (21.8%). A significant relationship was found between the carbapenemase-encoding gene (OXA-23 gene) and biofilm production. 4) Conclusion: to the best of our knowledge, this is the first study in Jordan that inspected CRAB virulence genes and highlighted the importance of improving infection control measures to avoid CRAB outbreaks.

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Emergence of mcr-1 gene and carbapenemase-encoding genes among colistin-resistant Klebsiella pneumoniae clinical isolates in Jordan

Cited by 16 publications

References 29 publications

Colistin Susceptibility among Carbapenem Resistant Klebsiella pneumoniae Isolated from Menoufia University Hospitals

Colistin Susceptibility among Carbapenem Resistant Klebsiella pneumoniae Isolated from Menoufia University Hospitals

Occurrence, virulence, and resistance genes in Salmonella enterica isolated from an integrated poultry company in Jordan

Virulence Molecular Epidemiology of Clinical Carbapenem-Resistant Acinetobacter baumannii: First Report from Jordan

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