Emergence of metallo-β-lactamase IMP-14 and VIM-2 inPseudomonas aeruginosaclinical isolates from a tertiary-level hospital in Thailand

Abstract

Seventy-five clinical isolates of Pseudomonas aeruginosa collected in a tertiary teaching hospital in Thailand were investigated for susceptibility to antimicrobials including imipenem. Metallo-β-lactamase (MBL) enzymes were detected by E-test MBL assay and PCR; class 1 integron genes were also detected by PCR. Strains positive for bla(IMP) and bla(VIM) genes were further characterized by DNA sequencing and examined for clonality by pulsed-field gel electrophoresis. High rates of resistance to anti-pseudomonal agents were found. MBL enzymes were found in 13 (17·3%) strains and 24 (32%) carried class 1 integron genes. Twelve of the latter strains harboured the bla(IMP-14) gene and one strain the bla(VIM-2) gene. All of the IMP-14 strains were identical or closely related suggesting clonal dissemination of these genes.

Prevalence of β-lactamase classes A, C, and D among clinical isolates of Pseudomonas aeruginosa from a tertiary-level hospital in Bangkok, Thailand

“…These data suggest that OXA-10 and VEB-1 are encoded by a gene located on a mobile element, such as a class 1 integron (Sanschagrin et al, 1995;Girlich et al, 2002;Strateva and Yordanov, 2009). We found that 32.00% of the class 1 integrons of 75 isolates in a study by Piyakul et al (2012) were also present in the clinical isolates in the current study. However, this study did not investigate other mechanisms that may be responsible for resistance to β-lactam such as the alteration or loss of porin protein, or the upregulation of efflux systems.…”

First Report of bla IMP-14 on a Plasmid Harboring Multiple Drug Resistance Genes in Escherichia coli Sequence Type 131

“…To date, bla IMP-14 has not been described in E. coli , to our knowledge, and has largely previously been reported in P. aeruginosa and Acinetobacter baumannii strains by several hospital centers in Thailand, in some cases as part of clonal outbreaks ( 22 – 25 ). Although bla IMP-14 is similarly associated with class 1 integrons in these cases, as in pEC732-IMP14, the wider plasmid contexts and sequences of these integrons in P. aeruginosa and A. baumannii strains have not been investigated.…”

A Genetic Screen Reveals Novel Targets to Render Pseudomonas aeruginosa Sensitive to Lysozyme and Cell Wall-Targeting Antibiotics

“…Recalcitrant P. aeruginosa infection remains a huge threat to public healthcare. Increasing numbers of metallo-β-lactamase-producing clinical isolates have been identified around the world (Corvec et al, 2006 ; Chin et al, 2011 ; Jovcic et al, 2011 ; Piyakul et al, 2012 ; Van der Bij et al, 2012 ), and strains exhibiting resistance to colistin, a “last hope” antibiotic, have also been isolated (Lee et al, 2014 ). Therefore, novel anti-Pseudomonas strategies that do not generate escape mutants are required.…”