Emergence of Multifunctional Oxygenase Activities by Random Priming Recombination

Suenaga, Hikaru; Goto, Minoru; Furukawa, Koichi

doi:10.1074/jbc.m101323200

Cited by 49 publications

(29 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Site-directed mutagenesis of naphthalene dioxygenase indicates that slight changes in amino acid sequence can have profound effects on reaction regio-and stereospecificity (681). Questions related to enzyme functionality and the evolution of similar naphthalene dioxygenases in different genera (e.g., Pseudomonas and Rhodococcus) will be answered as more enzymes are purified and characterized (93,237,310,368,414,361,472,591,592).…”

Section: Aerobic Pah Metabolismmentioning

confidence: 99%

Recent Advances in Petroleum Microbiology

Hamme¹,

Singh

Ward

2003

Microbiol Mol Biol Rev

1,201

640

View full text Add to dashboard Cite

Recent advances in molecular biology have extended our understanding of the metabolic processes related to microbial transformation of petroleum hydrocarbons. The physiological responses of microorganisms to the presence of hydrocarbons, including cell surface alterations and adaptive mechanisms for uptake and efflux of these substrates, have been characterized. New molecular techniques have enhanced our ability to investigate the dynamics of microbial communities in petroleum-impacted ecosystems. By establishing conditions which maximize rates and extents of microbial growth, hydrocarbon access, and transformation, highly accelerated and bioreactor-based petroleum waste degradation processes have been implemented. Biofilters capable of removing and biodegrading volatile petroleum contaminants in air streams with short substrate-microbe contact times (<60 s) are being used effectively. Microbes are being injected into partially spent petroleum reservoirs to enhance oil recovery. However, these microbial processes have not exhibited consistent and effective performance, primarily because of our inability to control conditions in the subsurface environment. Microbes may be exploited to break stable oilfield emulsions to produce pipeline quality oil. There is interest in replacing physical oil desulfurization processes with biodesulfurization methods through promotion of selective sulfur removal without degradation of associated carbon moieties. However, since microbes require an environment containing some water, a two-phase oil-water system must be established to optimize contact between the microbes and the hydrocarbon, and such an emulsion is not easily created with viscous crude oil. This challenge may be circumvented by application of the technology to more refined gasoline and diesel substrates, where aqueous-hydrocarbon emulsions are more easily generated. Molecular approaches are being used to broaden the substrate specificity and increase the rates and extents of desulfurization. Bacterial processes are being commercialized for removal of H2S and sulfoxides from petrochemical waste streams. Microbes also have potential for use in removal of nitrogen from crude oil leading to reduced nitric oxide emissions provided that technical problems similar to those experienced in biodesulfurization can be solved. Enzymes are being exploited to produce added-value products from petroleum substrates, and bacterial biosensors are being used to analyze petroleum-contaminated environments

show abstract

Section: Aerobic Pah Metabolismmentioning

confidence: 99%

Recent Advances in Petroleum Microbiology

Hamme¹,

Singh

Ward

2003

Microbiol Mol Biol Rev

1,201

640

View full text Add to dashboard Cite

show abstract

“…For example, mutagenesis of the catalytically active subunit of the dioxygenase may expand the substrate range of dioxin-degrading bacteria with dioxygenases. Recently, Suenaga et al 175) have shown that functional evolution of Pseudomonas pseudoalcaligenes biphenyl dioxygenase was accomplished by random priming recombination of the bphA1 gene. Interestingly, the evolved dioxygenase acquired multifunctional degradation capabilities not only for PCBs but also for DF, DD, DBT and FN.…”

Section: Concluding Remarks and Prospectsmentioning

confidence: 99%

Biodiversity of Dioxin-Degrading Microorganisms and Potential Utilization in Bioremediation.

Hiraishi

2003

Microb. Environ.

View full text Add to dashboard Cite

Dioxins are a group of chloroaromatic compounds known to be toxic and carcinogenic, and persistent environmental pollutants. How to remedy dioxin-polluted environments is one of the most challenging problems in environmental technology. From ecological and economical viewpoints, biological methods using particular microorganisms and microbial consortia capable of dioxin transformation and degradation have greater appeal than physicochemical methods in their application for environmental remediation. Large numbers of microorganisms capable of degrading dioxins and dioxin-like compounds have been isolated and characterized. Information about the biodiversity, ecophysiology and molecular biology of dioxin-degrading microorganisms has accumulated particularly during the past decade. There are three major modes of microbial degradation and transformation of dioxins; that is, oxidative degradation by aerobic bacteria containing aromatic hydrocarbon dioxygenases, reductive dechlorination by anaerobic microorganisms and fungal oxidation with cytochrome P-450, lignin peroxidases and laccases. This article overviews the current knowledge of microbial degradation and transformation of dioxins as well as the biodiversity of microorganisms involved. Strategies directed towards the bioremediation of dioxin-polluted environments are discussed.

show abstract

“…Biphenyl-utilizing bacteria are ubiquitously distributed and isolated from various environmental samples (3,10,27,38). These bacteria have been extensively studied with respect to the degradation of polychlorinated biphenyls (PCBs), a family of xenobiotic compounds that are major environmental pollutants.…”

mentioning

confidence: 99%

Alteration of Regiospecificity in Biphenyl Dioxygenase by Active-Site Engineering

et al. 2002

Self Cite

View full text Add to dashboard Cite

Structure 6:571-586, 1998), we developed a three-dimensional model of KF707 BphA1 of Pseudomonas pseudoalcaligenes KF707. Based on structural information about the amino acids which coordinate the catalytic nonheme iron center, we constructed 12 site-directed BphA1 mutants with changes at positions 227, 332, 335, 376, 377, and 383 and expressed these enzymes in Escherichia coli. The Ile335Phe, Thr376Asn, and Phe377Leu Bph Dox mutants exhibited altered regiospecificities for various PCBs compared with wild-type Bph Dox. In particular, the Ile335Phe mutant acquired the ability to degrade 2,5,2,5-tetrachlorobiphenyl by 3,4-dioxygenation and showed bifunctional 2,3-dioxygenase and 3,4-dioxygenase activities for 2,5,2-trichlorobiphenyl and 2,5,4-trichlorobiphenyl. Furthermore, two mutants, the Phe227Val and Phe377Ala mutants, introduced molecular oxygen at the 2,3 position, forming 3-chloro-2,3-dihydroxy biphenyl with concomitant dechlorination.

show abstract

Emergence of Multifunctional Oxygenase Activities by Random Priming Recombination

Cited by 49 publications

References 35 publications

Recent Advances in Petroleum Microbiology

Recent Advances in Petroleum Microbiology

Biodiversity of Dioxin-Degrading Microorganisms and Potential Utilization in Bioremediation.

Alteration of Regiospecificity in Biphenyl Dioxygenase by Active-Site Engineering

Contact Info

Product

Resources

About