Emergence of New Pathogenic Variants in Colletotrichum falcatum, Stalk Infecting Ascomycete in Sugarcane: Role of Host Varieties

Viswanathan, R.; Padmanaban, P.; Selvakumar, R.

doi:10.1007/s12355-019-00780-3

Cited by 25 publications

(11 citation statements)

References 15 publications

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“…To assess pathogenic variation amongst the isolates, the same reactions were categorized as LeV: least virulent (0–2.0), LV: less virulent (2.1–4.0), MV: moderately virulent (4.1–6.0), V: virulent (6.1–8.0) and HV: highly virulent (8.1–9.0) [ 28 ]. Finally, the pathogenic reactions were grouped as less virulent (LeV and LV), moderately (MV) and virulent (V and HV) for group comparison [ 29 ]. At the end of each season, the respective isolates were re-isolated from infected cane tissues; sub cultured and kept at 4 ± 1 °C for future use.…”

Section: Methodsmentioning

confidence: 99%

Phylogenetic Analysis and Genetic Diversity of Colletotrichum falcatum Isolates Causing Sugarcane Red Rot Disease in Bangladesh

Hossain

Ahmad

Vadamalai

et al. 2021

Biology

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Colletotrichum falcatum Went causes red rot disease in sugarcane farming in the tropical and sub-tropical regions. This disease causes significant economic loss to the sugarcane production industry. Successful disease management strategies depend on understanding the evolutionary relationship between pathogens, genetic diversity, and population structure, particularly at the intra-specific level. Forty-one isolates of C. falcatum were collected from different sugarcane farms across Bangladesh for molecular identification, phylogeny and genetic diversity study. The four genes namely, ITS-rDNA, β-tubulin, Actin and GAPDH sequences were conducted. All the 41 C. falcatum isolates showed a 99–100% similarity index to the conserved gene sequences in the GenBank database. The phylogram of the four genes revealed that C. falcatum isolates of Bangladesh clustered in the same clade and no distinct geographical structuring were evident within the clade. The four gene sequences revealed that C. falcatum isolates from Bangladesh differed from other countries´ isolates because of nucleotides substitution at different loci. The genetic structure of C. falcatum isolates were determined using ISSR marker generated 404 polymorphic loci from 10 selected markers. The percentage of polymorphic loci was 99.01. The genetic variability at species level was slightly higher than at population level. Total mean gene diversity at the species level was 0.1732 whereas at population level it was 0.1521. The cluster analysis divided 41 isolates into four main genetic groups and the principal component analysis was consistent with cluster analysis. To the best of our knowledge, this is the first finding on characterizing C. falcatum isolates infesting sugarcane in Bangladesh. The results of this present study provide important baseline information vis a vis C. falcatum phylogeny analysis and genetic diversity study.

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Section: Methodsmentioning

confidence: 99%

Phylogenetic Analysis and Genetic Diversity of Colletotrichum falcatum Isolates Causing Sugarcane Red Rot Disease in Bangladesh

Hossain

Ahmad

Vadamalai

et al. 2021

Biology

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“…Many fungal isolates are significantly different [13]. Diversity in virulence within pathotypes had revealed that a red rot pathogen undergoes adaptive changes in host cultivars.Viswanathan et al [19] reported that isolates are virulent in susceptible varieties, but not in resistant and moderately susceptible varieties. Virulence frequencies of isolates range from 21.3-40% on mildly susceptible varieties compared with 62.9-97.9% on susceptible varieties.…”

Section: Pathogen Infection and Transmissionmentioning

confidence: 99%

Current and Prospective Strategies on Detecting and Managing Colletotrichumfalcatum Causing Red Rot of Sugarcane

et al. 2020

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Sugarcane is an important industrial crop because it is the major source of white sugar. It is also one of the crops for the alcohol and biofuel industries. Disease-causing organisms can significantly decrease the productivity of sugarcane plants and sugar quality. Among the disease-causing organisms, Colletotrichum falcatum Went causes the most significant economic loss (5–50%) in the sugarcane production due to red rot disease. This loss results in only 31% sugar recovery. It is reported that C. falcatum can kill sugarcane plants. Currently, there is no sustainable way of preventing red rot disease from spreading in sugarcane plantations. Many popular sugarcane varieties are no longer used in sugarcane cultivation because of their susceptibility to C. falcatum. The objectives of this manuscript were to: (i) summarize existing approaches for the early detection of red rot disease and controlling techniques of red rot disease in the field and laboratory and (ii) assess red rot disease control effectiveness so as to propose better methods for mitigating the spread C. falcatum. If our proposition is adopted or practiced, it could significantly contribute to the mitigation of C. falcatum infection in the sugarcane industry. This could enable achieving sustainable cultivation of sugarcanes to guarantee the sustainability of the sugar industry in the tropics and the subtropics.

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“…The fungal pathogen exhibits enormous variation under fields conditions and the pathogenics variants emerge regularly in tune with deployment of new host varieties for cultivation making the resistant to susceptible referred as "varietal breakdown" (Viswanathan et al 2020b). So, already many sugarcane varieties were replaced due to their breakdown to a new pathogenic strain, so chemical control may be more one possibility in the management of red rot in sugarcane crop.…”

Section: Introductionmentioning

confidence: 99%

“…So, already many sugarcane varieties were replaced due to their breakdown to a new pathogenic strain, so chemical control may be more one possibility in the management of red rot in sugarcane crop. Given the losses caused by the disease, existence of pathogenic variation in C. falcatum and the emergence of new virulent pathotypes were documented over the decades (Viswanathan 2010;Viswanathan et al 2020b).…”

Section: Introductionmentioning

confidence: 99%

A Rapid Microtiter Assay to Evaluate Fungicide Sensitivity to Colletotrichum falcatum Isolates

Menezes¹

2022

IJAB

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Chemical control of sugarcane red rot, caused by Colletotrichum falcatum, forms part of integrated management of the disease. A rapid microtiter bioassay based on the colorimetric changes of resazurin dye was developed to evaluate the sensitivity of C. falcatum to the main chemical fungicide groups, including strobilurin, triazole, benzimidazole, isophthalonitrile and dithiocarbamate. There was no significant difference among the isolates in terms of growth inhibition for any of the active ingredients tested (α = 0.01). The C. falcatum isolates showed almost similar sensitivity to various fungicides. The active ingredients varied in relation to fungitoxicity. Doses that inhibited 50% of C. falcatum growth were calculated as a percentage of resazurin reduction due to various fungicides. The colorimetric method used to assess the fungitoxicity of active ingredients to C. falcatum, combined with resazurin, proved a fast practical and efficient method. © 2022 Friends Science Publishers

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Emergence of New Pathogenic Variants in Colletotrichum falcatum, Stalk Infecting Ascomycete in Sugarcane: Role of Host Varieties

Cited by 25 publications

References 15 publications

Phylogenetic Analysis and Genetic Diversity of Colletotrichum falcatum Isolates Causing Sugarcane Red Rot Disease in Bangladesh

Phylogenetic Analysis and Genetic Diversity of Colletotrichum falcatum Isolates Causing Sugarcane Red Rot Disease in Bangladesh

Current and Prospective Strategies on Detecting and Managing Colletotrichumfalcatum Causing Red Rot of Sugarcane

A Rapid Microtiter Assay to Evaluate Fungicide Sensitivity to Colletotrichum falcatum Isolates

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