Emergence of OXA-48-producing Klebsiella pneumoniae and the novel carbapenemases OXA-244 and OXA-245 in Spain

Oteo, Jesús; Hernández, Juan Manuel Herrera; Espasa, Mateu; Fleites, Ana; Sáez, David; Bautista, Verónica; Pérez-Vázquez, Marı́a; Fernández-García, Ma Dolores; Delgado‐Iribarren, Alberto; Sánchez-Romero, Isabel; García-Picazo, Luisa; Miguel, Ma Dolores; Solís, Sonia; Aznar, Esteban; Trujillo, Glòria; Mediavilla, Concepción; Fontanals, Dionísia; Rojo, Susana; Vindel, Ana; Campos, José

doi:10.1093/jac/dks383

Cited by 119 publications

(96 citation statements)

References 21 publications

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“…In addition, when the enzyme was cloned into sensitive E. coli reference strains, it resulted in only modest increases in MICs of the carbapenems (Table 3) (62,64,65). Since 2001, 10 more named OXA-48 variants have been identified (Table 1), although it should be noted that a number of related unnamed enzymes have been identified in Shewanella species, thought to be the original host genus for this group of enzymes (66)(67)(68).…”

Section: Oxa-48 ␤-Lactamasementioning

confidence: 99%

OXA β-Lactamases

2014

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SUMMARY The OXA β-lactamases were among the earliest β-lactamases detected; however, these molecular class D β-lactamases were originally relatively rare and always plasmid mediated. They had a substrate profile limited to the penicillins, but some became able to confer resistance to cephalosporins. From the 1980s onwards, isolates of Acinetobacter baumannii that were resistant to the carbapenems emerged, manifested by plasmid-encoded β-lactamases (OXA-23, OXA-40, and OXA-58) categorized as OXA enzymes because of their sequence similarity to earlier OXA β-lactamases. It was soon found that every A. baumannii strain possessed a chromosomally encoded OXA β-lactamase (OXA-51-like), some of which could confer resistance to carbapenems when the genetic environment around the gene promoted its expression. Similarly, Acinetobacter species closely related to A. baumannii also possessed their own chromosomally encoded OXA β-lactamases; some could be transferred to A. baumannii , and they formed the basis of transferable carbapenem resistance in this species. In some cases, the carbapenem-resistant OXA β-lactamases (OXA-48) have migrated into the Enterobacteriaceae and are becoming a significant cause of carbapenem resistance. The emergence of OXA enzymes that can confer resistance to carbapenems, particularly in A. baumannii , has transformed these β-lactamases from a minor hindrance into a major problem set to demote the clinical efficacy of the carbapenems.

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Section: Oxa-48 ␤-Lactamasementioning

confidence: 99%

OXA β-Lactamases

2014

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“…T he class D ␤-lactamase OXA-48 (1) and its variants have emerged as important determinants of carbapenem resistance in Enterobacteriaceae, representing a public health concern in some countries (2)(3)(4). The objective of this study was to report a new OXA-48 variant.…”

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confidence: 99%

Detection of OXA-370, an OXA-48-Related Class D β-Lactamase, in Enterobacter hormaechei from Brazil

Sampaio

Ribeiro

Campos

et al. 2014

Antimicrob Agents Chemother

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“…A unique allelic profile was obtained with the seven isolates, corresponding to ST11. ST11 is an epidemic clone of K. pneumoniae that has been isolated from humans worldwide, and it is associated with the spread of resistance determinants such as OXA-48 or NDM (13,14,15). The MICs, determined and interpreted according to Clinical and Laboratory Standards Institute guidelines (16,17), showed high-level resistance to all 4,6-disubstituted 2-deoxystreptamine aminoglycosides, as well as to ampicillin, ceftazidime, sulfamethoxazole, tetracycline, chloramphenicol, and fluoroquinolones ( Table 2).…”

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confidence: 99%

Klebsiella pneumoniae Sequence Type 11 from Companion Animals Bearing ArmA Methyltransferase, DHA-1 β-Lactamase, and QnrB4

Hidalgo

Gutiérrez

Ovejero

et al. 2013

Antimicrob Agents Chemother

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Seven Klebsiella pneumoniae isolates from dogs and cats in Spain were found to be highly resistant to aminoglycosides, and ArmA methyltransferase was responsible for this phenotype. All isolates were typed by multilocus sequence typing (MLST) as ST11, a human epidemic clone reported worldwide and associated with, among others, OXA-48 and NDM carbapenemases. In the seven strains, armA was borne by an IncR plasmid, pB1025, of 50 kb. The isolates were found to coproduce DHA-1 and SHV-11 ␤-lactamases, as well as the QnrB4 resistance determinant. This first report of the ArmA methyltransferase in pets illustrates their importance as a reservoir for human multidrug-resistant K. pneumoniae.

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Emergence of OXA-48-producing Klebsiella pneumoniae and the novel carbapenemases OXA-244 and OXA-245 in Spain

Abstract: Multidrug-resistant K. pneumoniae producing OXA-48-like carbapenemase are emerging as important pathogens in Spain due to intra- and inter-hospital, clonal and non-clonal dissemination.

Cited by 119 publications

References 21 publications

OXA β-Lactamases

OXA β-Lactamases

Detection of OXA-370, an OXA-48-Related Class D β-Lactamase, in Enterobacter hormaechei from Brazil

Klebsiella pneumoniae Sequence Type 11 from Companion Animals Bearing ArmA Methyltransferase, DHA-1 β-Lactamase, and QnrB4

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