Emergence of Staphylococcus hominis Strains in General Infections

Voineagu, Lavinia; Braga, Victoria; Botnarciuc, Mihaela; Barbu, Adina; Tataru, Mirela

doi:10.2478/v10307-012-0016-8

Cited by 6 publications

(4 citation statements)

References 5 publications

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“…The same happens with S. hominis subsp. hominis that has been reported as a potential pathogen isolated in generalized infections (Voineagu et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Potentially pathogenic bacteria isolated from neglected air and surfaces in hospitals

Oliveira

Cunha

Cruz

et al. 2021

Braz. J. Pharm. Sci.

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Hospital infections (HI) are a serious public health problem in many countries. Several studies have identified strains correlating to surgical site infections, many with multi-resistance. The goals of this study was to quantify, to identify and to verify the resistance profile of microorganisms collected at two hospitals settings, and to alert health professionals how environmental contamination can influence hospital infection rates. For air sampling in operating rooms, intensive care unit and materials sterilization center, the impaction method (Spin Air, IUL®) and passive sedimentation were used. For the isolation of bacteria on surfaces and uniforms contact plates (RODAC®) were used. Identification of the microorganisms was performed using Vitek® 2 Systems. The antibiograms were conducted according to the disk diffusion method recommended by CLSI. The surgical center of hospital B presented more than 500 CFU/m 3 in aerial microbial load. In the aerial microbiota of the sampled areas of both hospitals, M. luteus, S. haemolyticus and S. hominis spp hominis were the prevalent microorganisms, with a percentage greater than 30%. On the surfaces and uniforms there was a prevalence of M. luteus (40%) and S. hominis spp hominis (20%) among others, and some of the resistant strains were isolated from environments with microbial load within the recommended limits.

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“…The same happens with S. hominis subsp. hominis that has been reported as a potential pathogen isolated in generalized infections (Voineagu et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Potentially pathogenic bacteria isolated from neglected air and surfaces in hospitals

Oliveira

Cunha

Cruz

et al. 2021

Braz. J. Pharm. Sci.

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“…soil [20] NDA decomposition of cellulose [4] Cellulosmicrobium cellulans soil [20] NDA biocorrosion of mineral building materials [4] Brevibacterium spp. water [21] NDA deterioration of concrete, brick, glass [4] Leifsonia aquatica water [22] acute septicemia [22] NDA; Leifsonia poae: deterioration of wooden altar [23] Staphylococcus haemolitycus skin [24,25] neonatal sepsis [24] deterioration of photographic paper [26] Staphylococcus hominis skin of arms, legs, trunk [27,28] general infections, sepsis [29] deterioration of photographic paper [26] Staphylococcus capitis skin [30] prosthetic joint infections [31] deterioration of stone [26] Staphylococcus spp. skin and mucous membranes [14] dependent on the species deterioration of stone and murals [32] Micrococcus spp.…”

Section: Bacterial Species Possible Sources Pathogenicity Biodeteriormentioning

confidence: 99%

Bioaerosols in an Underground Tourist Trail

Wlazło¹,

Kasela²,

Nowakowicz-Dębek³

et al. 2020

Pol. J. Environ. Stud.

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“…Among coagulase-negative staphylococci (CONs), S. homins is one of the three most identified isolates from the blood of hospitalized patients [ 2 ]. Studies have reported that among staphylococcal infections, 15% were caused by S. hominis and were also associated with several other diseases, including peritonitis, osteomyelitis, bone and joint infection, cancer, and bacterial meningitis [ 3 , 4 , 5 , 6 , 7 ]. S. hominis is a multidrug-resistant bacterium that is resistant to a class of antibiotics known as lactams (methicillin, tetracycline, erythromycin, novobiocin, and oxacillin).…”

Section: Introductionmentioning

confidence: 99%

Genome-Based Multi-Antigenic Epitopes Vaccine Construct Designing against Staphylococcus hominis Using Reverse Vaccinology and Biophysical Approaches

et al. 2022

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Staphylococcus hominis is a Gram-positive bacterium from the staphylococcus genus; it is also a member of coagulase-negative staphylococci because of its opportunistic nature and ability to cause life-threatening bloodstream infections in immunocompromised patients. Gram-positive and opportunistic bacteria have become a major concern for the medical community. It has also drawn the attention of scientists due to the evaluation of immune evasion tactics and the development of multidrug-resistant strains. This prompted the need to explore novel therapeutic approaches as an alternative to antibiotics. The current study aimed to develop a broad-spectrum, multi-epitope vaccine to control bacterial infections and reduce the burden on healthcare systems. A computational framework was designed to filter the immunogenic potent vaccine candidate. This framework consists of pan-genomics, subtractive proteomics, and immunoinformatics approaches to prioritize vaccine candidates. A total of 12,285 core proteins were obtained using a pan-genome analysis of all strains. The screening of the core proteins resulted in the selection of only two proteins for the next epitope prediction phase. Eleven B-cell derived T-cell epitopes were selected that met the criteria of different immunoinformatics approaches such as allergenicity, antigenicity, immunogenicity, and toxicity. A vaccine construct was formulated using EAAAK and GPGPG linkers and a cholera toxin B subunit. This formulated vaccine construct was further used for downward analysis. The vaccine was loop refined and improved for structure stability through disulfide engineering. For an efficient expression, the codons were optimized as per the usage pattern of the E coli (K12) expression system. The top three refined docked complexes of the vaccine that docked with the MHC-I, MHC-II, and TLR-4 receptors were selected, which proved the best binding potential of the vaccine with immune receptors; this was followed by molecular dynamic simulations. The results indicate the best intermolecular bonding between immune receptors and vaccine epitopes and that they are exposed to the host’s immune system. Finally, the binding energies were calculated to confirm the binding stability of the docked complexes. This work aimed to provide a manageable list of immunogenic and antigenic epitopes that could be used as potent vaccine candidates for experimental in vivo and in vitro studies.

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Emergence of Staphylococcus hominis Strains in General Infections

Cited by 6 publications

References 5 publications

Potentially pathogenic bacteria isolated from neglected air and surfaces in hospitals

Potentially pathogenic bacteria isolated from neglected air and surfaces in hospitals

Bioaerosols in an Underground Tourist Trail

Genome-Based Multi-Antigenic Epitopes Vaccine Construct Designing against Staphylococcus hominis Using Reverse Vaccinology and Biophysical Approaches

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