Emergence of the Chytrid Fungus<i>Batrachochytrium Dendrobatidis</i>and Global Amphibian Declines

Fisher, Matthew C.; Stajich, Jason E.; Farrer, Rhys A.

doi:10.1002/9781118308165.ch24

Cited by 4 publications

(3 citation statements)

References 45 publications

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“…The availability of whole genome sequences and analysis has enabled computational searches for genes that have contributed to the evolutionary transition to pathogenicity. During the initial analysis of Bd genome from two isolates, JEL423 and JAM81, the expansion of CBM18 gene family was identified, which implicated expansions as an important recent adaption in the transition to pathogenicity (Abramyan and Stajich, 2012;Fisher et al, 2012;Joneson et al, 2011;Rosenblum et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Carbohydrate Binding Module 18 gene family in the amphibian pathogen Batrachochytrium dendrobatidis

Liu

Stajich

2015

Fungal Genetics and Biology

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Section: Introductionmentioning

confidence: 99%

Characterization of the Carbohydrate Binding Module 18 gene family in the amphibian pathogen Batrachochytrium dendrobatidis

Liu

Stajich

2015

Fungal Genetics and Biology

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“…Reactions for the quantification of Bd load were run in singlicate 25 µL volumes comprising 5 µL of DNA (diluted 1:10) and 12.5 µL of TaqMan Fast Advanced Master Mix (Thermo Fisher Scientific, Inc.), 2.75 µL nuclease-free H 2 O, 0.625 µL of primer ITS1-3 Chytr (18 µM), 0.625 µL of primer 5.8S Chytr (18 µM), 0.625 µL of probe Chytr MGB2 (5 µM), and 0.50 µL bovine serum albumin (400 ng/µL) per reaction [40]. A standard curve was generated using the global panzootic strain JEL423 [41], which had a dynamic range of 0.1 to 1,000 zoospore equivalents (ZE). We considered samples Bd positive via qPCR if the load was greater than 1 ZE.…”

Section: Methodsmentioning

confidence: 99%

“…A standard curve was generated using the global panzootic strain JEL423 [41], which had a dynamic range of 0.1 to 1,000 zoospore equivalents (ZE). We considered samples Bd positive via qPCR if the load was greater than 1 ZE.…”

Section: Quantitative Polymerase Chain Reaction (Qpcr) For Bd Genotypingmentioning

confidence: 99%

Elevated prevalence of the global panzootic chytrid strain in Ecuadorian anurans of the Amazonian lowlands

Smart,

McCracken,

Brunner

et al. 2024

Preprint

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Considerable attention has been directed to studying the infection dynamics of the fungal pathogen,Batrachochytrium dendrobatidis(Bd), affecting amphibians in the high elevations of the Neotropics. Lowland forests of the same realm, on the other hand, remain relatively understudied in this context. Herein, an attempt to bridge this gap was made by investigating the occurrence ofBdin several anuran taxa inhabiting the Amazonian lowlands in the northeast of Ecuador. To this end, 207 anurans belonging to 10 different families, 25 different genera, and 55 distinct host species were sampled forBdDNA in 2008. Data on the taxonomy, morphology (i.e., weight and snout-vent length), and life-long aquatic dependency of hosts (i.e., aquatic index) were also collated to serve as potential predictors of infection prevalence. Genotyping via quantitative PCR revealed the presence of the global panzootic lineage ofBd(Bd-GPL) in the Ecuadorian Amazon. The overall infection prevalence ofBdwas determined to be 58%, which is a relatively high prevalence rate ofBdreported for any amphibian population from the lowlands of the Neotropics to date. A total of 88% of sampled anuran families tested positive for the infection at varying proportions. A logistic regression analysis showed a significant negative relationship between host weight and the proportion ofBdinfections (p < 0.05). However, no significant associations were observed between host taxonomy, aquatic dependency, or snout-vent length andBdprevalence. Our findings contribute to the understanding ofBddynamics in the Neotropical lowlands and emphasize the need for future research on the ecological factors influencingBdin the Amazon and their implications for amphibian conservation.

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Towards a rapid sequencing-based molecular surveillance and mosaicism investigation of Toxoplasma gondii

et al. 2020

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Emergence of the Chytrid FungusBatrachochytrium Dendrobatidisand Global Amphibian Declines

Cited by 4 publications

References 45 publications

Characterization of the Carbohydrate Binding Module 18 gene family in the amphibian pathogen Batrachochytrium dendrobatidis

Characterization of the Carbohydrate Binding Module 18 gene family in the amphibian pathogen Batrachochytrium dendrobatidis

Elevated prevalence of the global panzootic chytrid strain in Ecuadorian anurans of the Amazonian lowlands

Towards a rapid sequencing-based molecular surveillance and mosaicism investigation of Toxoplasma gondii

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