Emergence of the Uncommon Clone ST944/ST78 Carrying bla_OXA-40-like and bla_CTX-M-like Genes Among Carbapenem-Nonsusceptible Acinetobacter baumannii in Moscow, Russia

Abstract

Carbapenem-nonsusceptible (Carba-NS) Acinetobacter baumannii has emerged as an important cause of nosocomial infections. In the present study, we characterized 91 Carba-NS A. baumannii isolates collected from patients of surgical departments and intensive care units at three hospitals in Moscow in 2012-2015. Multilocus sequence typing (MLST) using the Oxford (Oxf) scheme identified 16 sequence types (STs) of three clonal complexes (CCs), including CC92 (67%), CC109 (1%), CC944 (29%), and the singleton ST1100 (3%). CC944 was composed of ST944 (n = 16) and two of its newly described single locus variants ST1103 (n = 3) and ST1104 (n = 7); all the three STs were identical to the Pasteur (Pas) MLST scheme ST78. All CC944/ST78 isolates were bla positive and all but one isolate harbored a bla gene. ST944 was the only ST found in each of the three study hospitals. Biofilm growth capacity was similar among Carba-NS and nonclonal carbapenem-susceptible isolates. Our data demonstrate the predominance of two clonal lineages among Carba-NS A. baumannii. One of these, the uncommon bla/bla-positive clone of CC944/ST78, seems to be endemic in Russia.

Insights into Acinetobacter baumannii: A Review of Microbiological, Virulence, and Resistance Traits in a Threatening Nosocomial Pathogen

“…Cefepime and carbapenems appear to be stable in the presence of these enzymes [155]. Whole-genome sequencing approaches are allowing detection of new AmpC alleles like AmpC-69, AmpC-70, and AmpC-71 [158]; the new AmpC allelic variant encoded by bla was also recently identified in a clinical A. baumannii isolate from China [159].…”

Genotypes and metallo-beta-lactamases carriage in carbapenem-resistant Pseudomonas aeruginosa isolated from children in Moscow

“…Отметим, что эпидемическая популяционная струк тура P. aeruginosa с МЛУфенотипом отличается от других бактерий, в частности, A. baumannii, популяция МЛУштаммов которых характеризуется выраженной клональностью [8,25].…”

Diversity of International High-Risk Clones of Acinetobacter baumannii Revealed in a Russian Multidisciplinary Medical Center during 2017–2019

“…Although many reports consider different clones or lineages of A. baumannii species to be associated with particular parts or regions of the world [ 13 , 20 , 21 , 22 ], the dramatically fast distribution of SARS-CoV-2 in 2020 has demonstrated that our knowledge regarding the spread of different pathogens is still limited.…”