2021
DOI: 10.3389/fmolb.2021.717771
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Emergent Differential Organization of Airway Smooth Muscle Cells on Concave and Convex Tubular Surface

Abstract: Airway smooth muscle cells (ASMCs) exist in a form of helical winding bundles within the bronchial airway wall. Such tubular tissue provides cells with considerable curvature as a physical constraint, which is widely thought as an important determinant of cell behaviors. However, this process is difficult to mimic in the conventional planar cell culture system. Here, we report a method to develop chips with cell-scale tubular (concave and convex) surfaces from fused deposition modeling 3D printing to explore h… Show more

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Cited by 8 publications
(8 citation statements)
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“…This contractility altered the migration and expression of cells with affected proliferation rate too. As the simple conclusion, depending on the cells phenotype expression of some proteins could be different on flat and curved surfaces, also proliferation and migration of cells, in this case, has been increased on convex and concave surfaces compared to flat ones, and the contraction switched to proliferation which some aspects have been clear in Figure 6a–f (Jin et al, 2021).…”
Section: Vascular Nerve Muscle and Intestine Cell Alignmentsmentioning
confidence: 91%
“…This contractility altered the migration and expression of cells with affected proliferation rate too. As the simple conclusion, depending on the cells phenotype expression of some proteins could be different on flat and curved surfaces, also proliferation and migration of cells, in this case, has been increased on convex and concave surfaces compared to flat ones, and the contraction switched to proliferation which some aspects have been clear in Figure 6a–f (Jin et al, 2021).…”
Section: Vascular Nerve Muscle and Intestine Cell Alignmentsmentioning
confidence: 91%
“…The procedure for the cell counting Kit-8 (CCK-8) assay kit (#C0037, Beyotime Biotechnology, Shanghai, China) was as described previously (Jin et al, 2021). Cells were plated in 96well plates at a density of 1×10 4 cells/well.…”
Section: Assessment Of Viability Of Asmcsmentioning
confidence: 99%
“…These results might explain the pathogenesis of the lung microvascular thrombosis and endotheliitis that existed in severe cases of SARS-CoV-2 infection. With the advances in microfabrication technology, microfluidics, and tissue engineering (Jin et al, 2021), new approaches to the development of lung-on-a-chip models enable the production of more robust and high-throughput human in vitro respiratory tract models. The organoplate is an organ-on-a-chip platform comprising 96 microchambers that can be used for 3D cell culture (Trietsch et al, 2013).…”
Section: Lung-on-a-chip Models To Mimic Epithelium-endothelium Interfacementioning
confidence: 99%
“…In addition to dynamic mechanical distortion of the alveolar-capillary interface, there is increasing evidence that the microscale curved surfaces affect the spatiotemporal organization and behaviors of cells ( Callens et al, 2020 ; Jin et al, 2021 ). To recreate the mainly spherical geometry of the cells’ native microenvironment, Baptista et al (2022 ) made the membranes the shape of hexagonally arrayed hemispherical microwells by using a combination of 3D microfilm forming and ion track technology.…”
Section: 3d Cell Culture Models To Mimic Lung In Study Of Sars-cov-2 ...mentioning
confidence: 99%