We discuss how to calculate non-equilibrium universal amplitude ratios in the functional renormalization group approach, extending its applicability. In particular, we focus on the critical relaxation of the Ising model with non-conserved dynamics (model A) and calculate the universal amplitude ratio associated with the fluctuation-dissipation ratio of the order parameter, considering a critical quench from a high-temperature initial condition. Our predictions turn out to be in good agreement with previous perturbative renormalization-group calculations and Monte Carlo simulations.