Emerging bioanalytical applications of DNA walkers

Mason, Sean D.; Tang, Yanan; Li, Yongya; Xie, Xiaoyu; Li, Feng

doi:10.1016/j.trac.2018.08.015

Cited by 116 publications

(81 citation statements)

References 60 publications

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“…Large bending stiffness of the rotary arms and high rigidity of the supporting platform are primary for realization of rotary motion. Therefore, multilayer DNA origami components are generally employed to construct the rotary apparatus, which is different from the case of translational motion that many examples are based on small flexible structures operating on DNA origami . It has been demonstrated the unpurified, 128 nm, 6‐helix DNA exhibit persistence lengths of ≈2.4 µm and can self‐assemble against a compression forces up to 4.7 pN, compared to the 50 nm persistence length of single helix of dsDNA …”

Section: Motion Behaviors Of Dna Nanomachinesmentioning

confidence: 99%

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Programming Motions of DNA Origami Nanomachines

Wang

Zhang

Liu

et al. 2019

Small

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DNA nanotechnology enables the precise fabrication of DNA‐based machines with nanoscale dimensions. A wide range of DNA nanomachines are designed, which can be activated by specific inputs to perform various movement and functions. The excellent rigidity and unprecedented addressability of DNA origami have made it an excellent platform for manipulating and investigating the motion behaviors of DNA machines at single‐molecule level. In this Concept, power supply, machine actuation, and motion behavior of DNA machines on origami platforms are summarized and classified. The strategies utilized for programming motion behavior of DNA machines on DNA origami are also discussed with representative examples. The challenges and outlook for future development of manipulating DNA nanomachines at the single molecule level are presented and discussed.

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Section: Motion Behaviors Of Dna Nanomachinesmentioning

confidence: 99%

“…We focus on the systems with DNA origami as the platform for manipulation and direct observation of DNA machine. For DNA machines employing DNA origami as the modular components, and fabrication and application of DNA dynamic nanosystems, readers are referred to recent reviews …”

Section: Introductionmentioning

confidence: 99%

Programming Motions of DNA Origami Nanomachines

Wang

Zhang

Liu

et al. 2019

Small

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“…The DNA walker is a DNA nanomachine that mimics natural molecule walkers such as dynein, myosin, and kinesin. DNA walkers were characterized by the gradual movement of nucleic acids along precious designed tracks . The DNA walker consisted of three basic structural components: a walker, tracks, and fuel molecules or other forms of energy inputs that triggered motion .…”

Section: Dna Nanomachinesmentioning

confidence: 99%

Micro/Nano Technology for Next‐Generation Diagnostics

Gao

Tang

et al. 2019

Small Methods

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There has been a constant and urgent need to upgrade the current diagnostic technologies to the next generation by utilizing innovative advanced technologies in order to meet new challenges. Micro/nano technology, a series of methods for design and manipulation materials at the micrometer and nanometer scales, which perfectly covers the key building blocks of life including cells, organelles, proteins, nucleic acids, and other components, has unique advantages over current diagnostic methods. For the applications of micro–nano technology in next‐generation diagnostics, three categories, including microchip‐based devices, DNA nanomachines, and DNA nanomaterials, are extensively reviewed. Specifically, for the microchip‐based devices, terahertz technology and surface acoustic wave technology show their ultrasensitivity of label‐free, amplification‐free diagnosis. For the DNA nanomachines, DNA tweezers, DNA robots, and DNA walkers exhibit a great potential for diagnosis with the arbitrary structures and controllable motion behaviors. For the DNA nanomaterials, DNA aptamers, DNAzymes, and hybrid DNA nanomaterials provide sensitive biorecognition elements and versatile signal transductions, which are demonstrated as promising material candidates for the next‐generation diagnostics. Through this review, it is envisioned that micro/nano technologies will play an important role in the next‐generation diagnostics.

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“…By rational design, assembled DNA structures could be integrated for “nanowalkers”, “nanotweezers”, and “nanogears”, When an external stimuli was introduced, the operation of these DNA nanomachines usually involved repeated steps of DNA conformation changes, thus generating amplified outputs. In some cases, a metal‐dependent DNAzyme was employed to drive the operation of the DNA nanomachine . Similar to protein enzymes, DNAzymes are able to catalyze sequence‐specific RNA cleavage with high efficiency.…”

Section: Introductionmentioning

confidence: 99%

“…In some cases, am etal-dependent DNAzymew as employed to drive the operation of the DNA nanomachine. [5] Similar to protein enzymes,D NAzymes are able to catalyzes equence-specific RNA cleavage with high efficiency.M ore importantly,D NAzymes are more stable and need simpler modificationp rocedures,r ivaling that of protein enzymes. [6] By taking advantage of these inherentf eatures, DNA nanomachinesh ave also been designed for the detection of various biomolecules.…”

Section: Introductionmentioning

confidence: 99%

Designing DNAzyme‐Powered Nanomachines Simultaneously Responsive to Multiple MicroRNAs

Yang

et al. 2018

Chemistry A European J

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Herein, a DNAzyme‐powered nanomachine responsive to multiple hepatocellular carcinoma (HCC)‐related miRNAs derived from clinical samples was designed. Initially, three types of nanomachines were constructed with dye molecule [(fluorescein (FAM), tetramethylrhodamin (TMR), and Cyanine 5 (Cy5)]‐labeled DNA–RNA chimeric substrates and a specific recognized probe for the corresponding miRNAs target. Once the target miRNAs were captured by two recognizing probes, the DNA nanomachine was initiated, leading to the hybridization between the DNAzyme and the substrates. With the help of a cofactor, the automatic operation of the nanomachine was driven by cyclic cleavage of the DNAzyme. Meanwhile, we also explored the recognition behavior between the recognizing probe and the target miRNA. Subsequently, these DNAzyme‐powered nanomachines were developed for the homogeneous and simultaneous detection of three target miRNAs at the femtomloar level. Furthermore, the potential in clinical diagnosis was proven by the successful determination of target miRNA in real clinical samples. Thus, this nanomachine‐based strategy possesses significant potential to be an innovation in miRNA analysis methodology.

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Emerging bioanalytical applications of DNA walkers

Cited by 116 publications

References 60 publications

Programming Motions of DNA Origami Nanomachines

Programming Motions of DNA Origami Nanomachines

Micro/Nano Technology for Next‐Generation Diagnostics

Designing DNAzyme‐Powered Nanomachines Simultaneously Responsive to Multiple MicroRNAs

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