Emerging Developments in Separation Techniques and Analysis of Chiral Pharmaceuticals

Abstract

Chiral separation, the process of isolating enantiomers from a racemic mixture, holds paramount importance in diverse scientific disciplines. Using chiral separation methods like chromatography and electrophoresis, enantiomers can be isolated and characterized. This study emphasizes the significance of chiral separation in drug development, quality control, environmental analysis, and chemical synthesis, facilitating improved therapeutic outcomes, regulatory compliance, and enhanced industrial processes. Capillary electrophoresis (CE) has emerged as a powerful technique for the analysis of chiral drugs. This review also highlights the significance of CE in chiral drug analysis, emphasizing its high separation efficiency, rapid analysis times, and compatibility with other detection techniques. High-performance liquid chromatography (HPLC) has become a vital technique for chiral drugs analysis. Through the utilization of a chiral stationary phase, HPLC separates enantiomers based on their differential interactions, allowing for the quantification of individual enantiomeric concentrations. This study also emphasizes the significance of HPLC in chiral drug analysis, highlighting its excellent resolution, sensitivity, and applicability. The resolution and enantiomeric analysis of nonsteroidal anti-inflammatory drugs (NSAIDs) hold great importance due to their chiral nature and potential variations in pharmacological effects. Several studies have emphasized the significance of resolving and analyzing the enantiomers of NSAIDs. Enantiomeric analysis provides critical insights into the pharmacokinetics, pharmacodynamics, and potential interactions of NSAIDs, aiding in drug design, optimization, and personalized medicine for improved therapeutic outcomes and patient safety. Microfluidics systems have revolutionized chiral separation, offering miniaturization, precise fluid control, and high throughput. Integration of microscale channels and techniques provides a promising platform for on-chip chiral analysis in pharmaceuticals and analytical chemistry. Their applications in techniques such as high-performance liquid chromatography (HPLC) and capillary electrochromatography (CEC) offer improved resolution and faster analysis times, making them valuable tools for enantiomeric analysis in pharmaceutical, environmental, and biomedical research.

Enantioselective separation and determination of ibuprofen: Stereoselective pharmacokinetics, pharmacodynamics and analytical methods

“…Inside the reactor, a mixture is stirred at a rate of 1100 1/min using a stir bar (6) and a magnetic stirrer (7). After the rapid decompression, carbon dioxide flows through the exit valve (10) into 40 mL of methanol (11) to capture the constituents saturated in carbon dioxide.…”

“…The presence of molecular chirality plays a crucial role in the interactions between medicinal compounds and biological receptors. [5][6][7][8] This significance arises from the fact that the majority of biological receptors, such as enzymes, transporters, and membrane proteins, are comprised of chiral molecules. Consequently, receptors often have the ability to differentiate between different forms of chiral drug stereoisomers, resulting in significantly varying effects on the body in terms of their pharmacological and toxicological impact.…”

“…The presence of molecular chirality plays a crucial role in the interactions between medicinal compounds and biological receptors 5–8 . This significance arises from the fact that the majority of biological receptors, such as enzymes, transporters, and membrane proteins, are comprised of chiral molecules.…”

Current Trends and Technological Advancements in the Study of Honey Bee-Derived Peptides with an Emphasis on State-of-the-Art Approaches: A Review

“…For these reasons, silica is frequently used as the material for the particles. It is also important to keep in mind that separation performance is also influenced by the quality of the silica that is used in HPLC columns [58,59].…”