Emerging drugs for chemotherapy-induced mucositis

Keefe, Dorothy; Sonis, Stephen T.; Bowen, Joanne M.

doi:10.1517/14728214.13.3.511

Cited by 43 publications

(17 citation statements)

References 65 publications

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“…Caco-2 human colorectal adenocarcinoma cells (passage [25][26][27][28][29][30][31][32][33][34][35] were cultured in medium containing DMEM supplemented with 10% (v/v) fetal calf serum (FCS) and 1% (v/v) antibiotics (penicillin, gentamicin, and streptomycin, Gibco Ò , Life Technologies, Mulgrave, Victoria, Australia). All cells were maintained at 37 C in a humidified incubator with 5% CO 2 and grown in 75 cm 2 vented tissue culture flasks (CELLSTAR Ò , Greiner Bio-One, Frankenhausen, Germany).…”

Section: Cell Culturementioning

confidence: 99%

Factors Derived From Escherichia Coli Nissle 1917, Grown in Different Growth Media, Enhance Cell Death in a Model of 5-Fluorouracil-Induced Caco-2 Intestinal Epithelial Cell Damage

Wang

Bastian

Lawrence

et al. 2015

Nutrition and Cancer

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We evaluated supernatants (SNs) from Escherichia coli Nissle 1917 (EcN) grown in commonly used growth media for their capacity to affect the viability of Caco-2 colon cancer cells in the presence and absence of 5-Fluorouracil (5-FU) chemotherapy. EcN was grown in Luria-Bertani (LB), tryptone soya (TSB), Man Rogosa Sharpe (MRS), and M17 broth supplemented with 10% (v/v) lactose solution (M17). Human Caco-2 colon cancer cells were treated with DMEM (control), growth media alone (LB, TSB, MRS, and M17) or EcN SNs derived from these 4 media, in the presence and absence of 5-FU. Cell viability, reactive oxygen species (ROS), and cell monolayer permeability were determined. EcN SN in LB medium reduced Caco-2 cell viability significantly, to 51% at 48 h. The combination of this EcN SN and 5-FU further reduced cell viability to 37% at 48 h, compared to 5-FU control. MRS broth and EcN SN in MRS, together with 5-FU, generated significantly lower levels of ROS compared to 5-FU control. However, all 5-FU treatments significantly disrupted the Caco-2 cell barrier compared to control; with no significant differences observed among any of the 5-FU treatments. EcN SNs (LB+) was most effective at decreasing the viability of Caco-2 cells. This could indicate a potential role for this EcN SN in chemoprevention for colon cancer.

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Section: Cell Culturementioning

confidence: 99%

Factors Derived From Escherichia Coli Nissle 1917, Grown in Different Growth Media, Enhance Cell Death in a Model of 5-Fluorouracil-Induced Caco-2 Intestinal Epithelial Cell Damage

Wang

Bastian

Lawrence

et al. 2015

Nutrition and Cancer

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“…It can occur to any mucosal cells, but most commonly occurs in the cells of the oral cavity and small intestine [5]. Oral mucositis caused by cytotoxic chemotherapy is characterized by painful inflammation, erythema, swelling, and ulcerations of the oral mucosa in the oral cavity, oropharynx, and hypopharynx [8,9].…”

Section: Mucositis and Stomatitismentioning

confidence: 99%

“…As reviewed by Elting et al [28], mucositis can lead to a dose reduction of chemotherapy in 23%-28% of cycles. These dose reductions are a major source of concern because they can directly diminish cure rates and patient survival [5].…”

Section: Impact On Patientsmentioning

confidence: 99%

“…For example, oral toxicities are a common cause of dose delays and interruptions of cancer therapy [4]. Unfortunately, the currently available treatments for oral toxicities are primarily palliative and in many cases, only marginally effective [5][6][7]. The lack of effective treatments and prevention strategies may be attributed to the fact that research on oral toxicities has lagged behind research on other chemotherapy and radiation-related toxicities such as febrile neutropenia and nausea and vomiting [4].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Oral Mucositis and Stomatitis Associated with Conventional and Targeted Anticancer Therapy

Parkhill¹

2013

J Pharmacovigilance

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Oral mucositis and stomatitis are characterized by pain, inflammation, and redness in the oral cavity. In the case of mucositis, ulceration also occurs. Despite being understudied, these symptoms are some of the most troubling, and yet common, adverse effects associated with cancer treatment. The incidence of these toxicities is highly variable depending on treatment-related and patient-related risk factors. However, most cancer treatment regimens carry some risk of this toxicity. The presence of mucositis or stomatitis can result in a significant decrease in the patient's quality of life due to severe pain and the inability to eat or drink. These toxicities can also negatively affect the overall outcome of cancer treatment, leading to dose reductions and delays in treatment. Although the mechanism is not yet understood, the rapid increase in the number of targeted anticancer therapies available on the market has increased the number of patients who experience oral toxicities. The toxicities caused by targeted agents are less severe than those caused by conventional anticancer agents, but due to prolonged dosing schedules, they can still lead to significant declines in quality of life. The pathobiology of oral mucositis is a complex five phase process. It involves injury, activation of enzymes and transcription factors, upregulation of cytokine genes, inflammation/damage to tissue, and healing. These processes not only involve cells in the oral mucosa, but also the surrounding fibroblasts and connective tissue cells. A better understanding of the mechanisms behind oral mucositis and stomatitis will facilitate the development of effective prediction, prevention, and treatment strategies.

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“…It presents debilitating symptoms as intestinal and/or oral pain, vomiting, diarrhea, sore mouth and destruction of the gastrointestinal epithelium that contributes to poor absorption of nutrients and, consequently, weight loss [2]. This scenario might force a reduction in the chemotherapeutics dose leading to a worse prognosis [3,4]. In addition, mucositis has a considerable economic impact, due to high costs with patients' hospitalization in order to manage symptoms characteristic of this disorder.…”

Section: Introductionmentioning

confidence: 99%