Emerging Effects of Resveratrol Derivatives in Cells Involved in Oral Wound Healing: A Preliminary Study

Abstract

Recently, there has been an increasing interest in finding new approaches to manage oral wound healing. Although resveratrol (RSV) exhibited many biological properties, such as antioxidant and anti-inflammatory activities, its use as a drug is limited by unfavorable bioavailability. This study aimed to investigate a series of RSV derivatives (1a–j) with better pharmacokinetic profiles. At first, their cytocompatibility at different concentrations was tested on gingival fibroblasts (HGFs). Among them, derivatives 1d and 1h significantly increased cell viability compared to the reference compound RSV. Thus, 1d and 1h were investigated for cytotoxicity, proliferation, and gene expression in HGFs, endothelial cells (HUVECs), and oral osteoblasts (HOBs), which are the main cells involved in oral wound healing. For HUVECs and HGFs, the morphology was also evaluated, while for HOBs ALP and mineralization were observed. The results showed that both 1d and 1h did not exert negative effects on cell viability, and at a lower concentration (5 µM) both even significantly enhanced the proliferative rate, compared to RSV. The morphology observations pointed out that the density of HUVECs and HGFs was promoted by 1d and 1h (5 µM) and mineralization was promoted in HOBs. Moreover, 1d and 1h (5 µM) induced a higher eNOS mRNA level in HUVECs, higher COL1 mRNA in HGFs, and higher OCN in HOBs, compared to RSV. The appreciable physicochemical properties and good enzymatic and chemical stability of 1d and 1h, along with their promising biological properties, provide the scientific basis for further studies leading to the development of RSV-based agents useful in oral tissue repair.

Complex magnetic fields represent an eco-sustainable technology to counteract the resistant Candida albicans growth without affecting the human gingival fibroblasts

“…To determine how CMFs affected the density and shape of the cells, toluidine-blue staining was employed 42 . According to the experimental plan, 2 × 10 4 cells/well were seeded in 24-well plates and were subjected to CMFs.…”

The Application of Resveratrol Derivatives in Oral Cells Reduces the Oxidative Stress Induced by Glucocorticoids

“…The versatile structure of sulfonamides and the wide range of their activities have contributed to increased interest in repurposing old drugs. In this field, we investigated a series of RSV derivatives with better pharmacokinetic profiles [30]. In previous work, we studied the effects of a set of stilbene-containing sulfonamides [30].…”

“…In this field, we investigated a series of RSV derivatives with better pharmacokinetic profiles [30]. In previous work, we studied the effects of a set of stilbene-containing sulfonamides [30]. These compounds can be considered derivatives of RSV with improved lipophilicity thanks to the presence of aromatic or aliphatic structural portions (Figure 1) [24].…”

“…bolites 2024, 14, x FOR PEER REVIEW 3 of 17 respect to control and RSV. They showed an upregulation of eNOS for endothelial cells of COL1 for gingival fibroblasts, and of ALP for osteoblasts, at the lowest concentration [30] [doi: 10.3390/ijms24043276]. They also showed favorable physicochemical properties enzymatic and chemical stability, and good wound-healing properties [30].…”

“…They showed an upregulation of eNOS for endothelial cells of COL1 for gingival fibroblasts, and of ALP for osteoblasts, at the lowest concentration [30] [doi: 10.3390/ijms24043276]. They also showed favorable physicochemical properties enzymatic and chemical stability, and good wound-healing properties [30]. Since structural analogy, derivatives can act in the same way as referent compounds with better results in scavenging ROS.…”