Emerging functions of PRKDC in the initiation and progression of cancer

Yin, Yuting; He, Qinglian; Li, Yuling; Long, Jiali; Xue, Lijun; Li, Ziqi; Zhu, Wei

doi:10.1177/0300891620950472

Cited by 5 publications

(4 citation statements)

References 52 publications

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“…Further analyses showed that SPP1 significantly contributed to tumor differentiation. SPP1 was discovered to be upregulated in undifferentiated tumors in Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC) [ 18 – 20 ]. It was encouraging that SPP1 is related to clinical-pathologic variables at the mRNA level, and PRKDC with increased SPP1 expression could progress to an advanced stage.…”

Section: Discussionmentioning

confidence: 99%

Higher Expression of SPP1 Predicts Poorer Survival Outcomes in Head and Neck Cancer

Bie

Zhang

2021

Journal of Immunology Research

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Secreted phosphoprotein 1 (SPP1) participated in various biological processes in many cancers, including immune response, tumor progression, and prognosis. However, SPP1 in head and neck squamous cell carcinoma (HNSCC) remains unknown. Clinical-genetic data of HNSCC were obtained from The Cancer Genome Atlas (TCGA) database. The differential expression of SPP1 in HNSCC tissues and adjacent normal tissues was quantified by bioinformatics methods and verified by western blot and other differential biological methods. We concluded that SPP1 is significantly upregulated in tumor tissues and can become a prognostic biomarker for HNSCC.

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Section: Discussionmentioning

confidence: 99%

Higher Expression of SPP1 Predicts Poorer Survival Outcomes in Head and Neck Cancer

Bie

Zhang

2021

Journal of Immunology Research

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“…Thus, it suggests that the inhibition of TTK by MS13 treatment may inhibit cell proliferation and induce apoptosis in AIPC cells. PRKDC is a critical component of DNA repair machinery, and its expression is implicated in tumour progression, metastasis and migration (Yin et al, 2020). The PRKDC knockdown has been reported to attenuate cell proliferation and metastasis in PCa cells (Goodwin et al, 2015;Zhang et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Diarylpentanoid (1,5-bis(4-hydroxy-3-methoxyphenyl)-1,4-pentadiene-3-one) (MS13) Exhibits Anti-proliferative, Apoptosis Induction and Anti-migration Properties on Androgen-independent Human Prostate Cancer by Targeting Cell Cycle–Apoptosis and PI3K Signalling Pathways

et al. 2021

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Diarylpentanoids exhibit a high degree of anti-cancer activity and stability in vitro over curcumin in prostate cancer cells. Hence, this study aims to investigate the effects of a diarylpentanoid, 1,5-bis(4-hydroxy-3-methoxyphenyl)-1,4-pentadiene-3-one (MS13) on cytotoxicity, anti-proliferative, apoptosis-inducing, anti-migration properties, and the underlying molecular mechanisms on treated androgen-independent prostate cancer cells, DU 145 and PC-3. A cell viability assay has shown greater cytotoxicity effects of MS13-treated DU 145 cells (EC50 7.57 ± 0.2 µM) and PC-3 cells (EC50 7.80 ± 0.7 µM) compared to curcumin (EC50: DU 145; 34.25 ± 2.7 µM and PC-3; 27.77 ± 6.4 µM). In addition, MS13 exhibited significant anti-proliferative activity against AIPC cells compared to curcumin in a dose- and time-dependent manner. Morphological observation, increased caspase-3 activity, and reduced Bcl-2 protein levels in these cells indicated that MS13 induces apoptosis in a time- and dose-dependent. Moreover, MS13 effectively inhibited the migration of DU 145 and PC-3 cells. Our results suggest that cell cycle-apoptosis and PI3K pathways were the topmost significant pathways impacted by MS13 activity. Our findings suggest that MS13 may demonstrate the anti-cancer activity by modulating DEGs associated with the cell cycle-apoptosis and PI3K pathways, thus inhibiting cell proliferation and cell migration as well as inducing apoptosis in AIPC cells.

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“…Among them, the two RBPs with the highest mutation frequency, PRKDC and HELZ2, were searched on the cBioportal platform. The PRKDC gene encodes the catalytic subunit of DNA-dependent protein kinase (DNA-PK) [ 29 ]. Together with Ku70/Ku80 heterodimeric protein, PRKDC has an effect on DNA double-strand break repair and recombination [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

The underlying molecular mechanisms and prognostic factors of RNA binding protein in colorectal cancer: a study based on multiple online databases

Xue

et al. 2021

Cancer Cell Int

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Background RNA binding protein (RBP) is an active factor involved in the occurrence and development of colorectal cancer (CRC). Therefore, the potential mechanism of RBP in CRC needs to be clarified by dry-lab analyses or wet-lab experiments. Methods The differential RBP gene obtained from the GEPIA 2 (Gene Expression Profiling Interactive Analysis 2) were performed functional enrichment analysis. Then, the alternative splicing (AS) events related to survival were acquired by univariate regression analysis, and the correlation between RBP and AS was analyzed by R software. The online databases were conducted to analyze the mutation and methylation of RBPs in CRC. Moreover, 5 key RBP signatures were obtained through univariate and multivariate Cox regression analysis and established as RBP prognosis model. Subsequently, the above model was verified through another randomized group of TCGA CRC cohorts. Finally, multiple online databases and qRT-PCR analysis were carried to further confirm the expression of the above 5 RBP signatures in CRC. Results Through a comprehensive bioinformatics analysis, it was revealed that RBPs had genetic and epigenetic changes in CRC. We obtained 300 differentially expressed RBPs in CRC samples. The functional analysis suggested that they mainly participated in spliceosome. Then, a regulatory network for RBP was established to participate in AS and DDX39B was detected to act as a potentially essential factor in the regulation of AS in CRC. Our analysis discovered that 11 differentially expressed RBPs with a mutation frequency higher than 5%. Furthermore, we found that 10 differentially expressed RBPs had methylation sites related to the prognosis of CRC, and a prognostic model was constructed by the 5 RBP signatures. In another randomized group of TCGA CRC cohorts, the prognostic performance of the 5 RBP signatures was verified. Conclusion The potential mechanisms that regulate the aberrant expression of RBPs in the development of CRC was explored, a network that regulated AS was established, and the RBP-related prognosis model was constructed and verified, which could improve the individualized prognosis prediction of CRC.

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Emerging functions of PRKDC in the initiation and progression of cancer

Cited by 5 publications

References 52 publications

Higher Expression of SPP1 Predicts Poorer Survival Outcomes in Head and Neck Cancer

Higher Expression of SPP1 Predicts Poorer Survival Outcomes in Head and Neck Cancer

Diarylpentanoid (1,5-bis(4-hydroxy-3-methoxyphenyl)-1,4-pentadiene-3-one) (MS13) Exhibits Anti-proliferative, Apoptosis Induction and Anti-migration Properties on Androgen-independent Human Prostate Cancer by Targeting Cell Cycle–Apoptosis and PI3K Signalling Pathways

The underlying molecular mechanisms and prognostic factors of RNA binding protein in colorectal cancer: a study based on multiple online databases

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