Emerging Yr26-Virulent Races of Puccinia striiformis f. tritici Are Threatening Wheat Production in the Sichuan Basin, China

Han, Dejun; Wang, Q. L.; Chen, X. M.; Zeng, Qingdong; Wu, Jianhui; Xue, Wei; Zhan, Gangming; Huang, Lili; Kang, Zhensheng

doi:10.1094/pdis-08-14-0865-re

Cited by 92 publications

(48 citation statements)

References 33 publications

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“…Arrows indicate wheat-rye 1BL•1RS translocation chromosomes . Han et al (2015) concluded that more than 76 % of wheat cultivars and breeding lines from the Sichuan Basin are susceptible to the V26/Gui 22 race at the seeding stage. It is, therefore, urgent to identify new stripe rust resistance genes and to use more effective genes to counterbalance the continuous evolution of rust pathogens in wheat-breeding programs.…”

Section: Discussionmentioning

confidence: 99%

Molecular cytogenetic characterization of a new wheat-rye 1BL•1RS translocation line expressing superior stripe rust resistance and enhanced grain yield

Yao

Zhu

et al. 2016

Planta

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A new wheat-rye 1BL•1RS translocation line, with the characteristics of superior stripe rust resistance and high thousand-kernel weight and grain number per spike, was developed and identified from progenies of wheat-rye- Psathyrostachys huashanica trigeneric hybrids. The wheat-rye 1BL•1RS translocation line from Petkus rye has contributed substantially to the world wheat production. However, due to extensive growing of cultivars with disease resistance genes from short arm of rye chromosome 1R and coevolution of pathogen virulence and host resistance, these cultivars successively lost resistance to pathogens. In this study, a new wheat-rye line K13-868, derived from the progenies of wheat-rye-Psathyrostachys huashanica trigeneric hybrids, was identified and analyzed using fluorescence in situ hybridization (FISH), genomic in situ hybridization (GISH), acid polyacrylamide gel electrophoresis (A-PAGE), and molecular markers. Cytological studies indicated that the mean chromosome configuration of K13-868 at meiosis was 2n = 42 = 0.14 I + 18.78 II (ring) + 2.15 II (rod). Sequential FISH and GISH results demonstrated that K13-868 was a compensating wheat-rye 1BL•1RS Robertsonian translocation line. Acid PAGE analysis revealed that clear specific bands of rye 1RS were expressed in K13-868. Simple sequence repeat (SSR) and rye 1RS-specific markers ω-sec-p1/ω-sec-p2 and O-SEC5'-A/O-SEC3'-R suggested that the 1BS arm of wheat had been substituted by the 1RS arm of rye. At the seedling and adult growth stage, compared with its recurrent wheat parent SM51 and six other wheat cultivars containing the 1RS arm in southwestern China, K13-868 showed high levels of resistance to stripe rust (Puccinia striiformis f. sp. tritici, Pst) pathogens prevalent in China, which are virulent to Yr10 and Yr24/Yr26. In addition, K13-868 expresses higher thousand-kernel weight and more grain number per spike than these controls in two growing seasons, suggesting that this line may carry yield-related genes of rye. This translocation line, with significant characteristics of resistance to stripe rust and high thousand-kernel weight and grain number per spike, could be utilized as a valuable germplasm for wheat improvement.

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Section: Discussionmentioning

confidence: 99%

Molecular cytogenetic characterization of a new wheat-rye 1BL•1RS translocation line expressing superior stripe rust resistance and enhanced grain yield

Yao

Zhu

et al. 2016

Planta

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“…Wheat stripe rust is one of the most serious wheat diseases. Therefore, stripe rust resistance gene Yr26 has been widely used in wheat breeding programs since its discovery in the early 1990s [1]. However, dependence on a single gene can be risky, as the rise of new virulent strains (such as the Yr26 -virulent race V26/Gui 22) can easily lead to a significant decline in wheat production.…”

Section: Introductionmentioning

confidence: 99%

“…However, dependence on a single gene can be risky, as the rise of new virulent strains (such as the Yr26 -virulent race V26/Gui 22) can easily lead to a significant decline in wheat production. Yr26 virulence may represent a major threat to wheat production in the Sichuan Basin and other regions of China [1]. The use of cultivars harboring novel resistance is an efficient and economical alternative to control wheat stripe rust [2].…”

Section: Introductionmentioning

confidence: 99%

Cytogenetics and stripe rust resistance of wheat–Thinopyrum elongatum hybrid derivatives

Long

et al. 2018

Mol Cytogenet

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BackgroundAmphidiploids generated by distant hybridization are commonly used as genetic bridge to transfer desirable genes from wild wheat species into cultivated wheat. This method is typically used to enhance the resistance of wheat to biotic or abiotic stresses, and to increase crop yield and quality. Tetraploid Thinopyrum elongatum exhibits strong adaptability, resistance to stripe rust and Fusarium head blight, and tolerance to salt, drought, and cold.ResultsIn the present study, we produced hybrid derivatives by crossing and backcrossing the Triticum durum–Th. elongatum partial amphidiploid (Trititrigia 8801, 2n = 6× = 42, AABBEE) with wheat cultivars common to the Sichuan Basin. By means of cytogenetic and disease resistance analyses, we identified progeny harboring alien chromosomes and measured their resistance to stripe rust. Hybrid progenies possessed chromosome numbers ranging from 40 to 47 (mean = 42.72), with 40.0% possessing 42 chromosomes. Genomic in situ hybridization revealed that the number of alien chromosomes ranged from 1 to 11. Out of the 50 of analyzed lines, five represented chromosome addition (2n = 44 = 42 W + 2E) and other five were chromosome substitution lines (2n = 42 = 40 W + 2E). Importantly, a single chromosome derived from wheat–Th. elongatum intergenomic Robertsonian translocations chromosome was occurred in 12 lines. Compared with the wheat parental cultivars (‘CN16’ and ‘SM482’), the majority (70%) of the derivative lines were highly resistant to strains of stripe rust pathogen known to be prevalent in China.ConclusionThe findings suggest that these hybrid-derivative lines with stripe rust resistance could potentially be used as germplasm sources for further wheat improvement.

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“…Cultivation of resistant varieties is the most effective, economical, and environmentally friendly way to control the disease. Although many resistance genes have been identified and utilized in wheat cultivars (Cheng et al, 2014; Lu et al, 2014; Han et al, 2015), the protection conferred has not been durable due to genetic variation in the pathogen population. One example is that of resistance gene Yr26 that was widely used in Chinese wheat breeding in recent years.…”

Section: Introductionmentioning

confidence: 99%

“…One example is that of resistance gene Yr26 that was widely used in Chinese wheat breeding in recent years. New Yr26 -virulent (CYR34, V26) races are now increasing and are causing unacceptable levels of disease on many of the cultivars with Yr26 (Han et al, 2015). Although resistances governed by quantitative trait loci (QTL) confer a broader-spectrum resistance, the level of resistance is not adequate to prevent significant crop losses, especially under severe epidemic conditions (Niks et al, 2015b).…”

Section: Introductionmentioning

confidence: 99%

Characterization and Genetic Analysis of Rice Mutant crr1 Exhibiting Compromised Non-host Resistance to Puccinia striiformis f. sp. tritici (Pst)

et al. 2016

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Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most devastating diseases of wheat in China. Rapid change to virulence following release of resistant cultivars necessitates ongoing discovery and exploitation of new resistance resources. Considerable effort has been directed at non-host resistance (NHR) which is believed to be durable. In the present study we identified rice mutant crr1 (compromised resistance to rust 1) that exhibited compromised NHR to Pst. Compared with wild type rice variety Nipponbare, crr1 mutant displayed a threefold increase in penetration rate by Pst, and enhanced hyphal growth. The pathogen also developed haustoria in crr1 mesophyll cells, but failed to sporulate. The response to the adapted rice pathogen Magnaporthe oryzae was unchanged in crr1 relative to the wild type. Several defense-related genes involved in the SA- and JA-mediated defense pathways response and in phytoalexin synthesis (such as OsPR1a, OsLOX1, and OsCPS4) were more rapidly and strongly induced in infected crr1 leaves than in the wild type, suggesting that other layers of defense are still in effect. Genetic analysis and mapping located the mutant loci at a region between markers ID14 and RM25792, which cover about 290 kb genome sequence on chromosome 10. Further fine mapping and cloning of the locus should provide further insights into NHR to rust fungi in rice, and may reveal new strategies for improving rust resistance in wheat.

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Emerging Yr26-Virulent Races of Puccinia striiformis f. tritici Are Threatening Wheat Production in the Sichuan Basin, China

Abstract: 21

Cited by 92 publications

References 33 publications

Molecular cytogenetic characterization of a new wheat-rye 1BL•1RS translocation line expressing superior stripe rust resistance and enhanced grain yield

Molecular cytogenetic characterization of a new wheat-rye 1BL•1RS translocation line expressing superior stripe rust resistance and enhanced grain yield

Cytogenetics and stripe rust resistance of wheat–Thinopyrum elongatum hybrid derivatives

Characterization and Genetic Analysis of Rice Mutant crr1 Exhibiting Compromised Non-host Resistance to Puccinia striiformis f. sp. tritici (Pst)

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