Emerging of<i>ptxP</i>3 lineage in<i>B</i>ordetella pertussis strains circulating in a population in northeastern Mexico

Gutiérrez-Ferman, Jessica Lizzeth; Villarreal-Treviño, Licet; Ramírez-Aranda, José Manuel; Camacho-Ortíz, Adrián; Ballesteros-Elizondo, María Romelia; Moreno-Juárez, M R; Mendoza-Olazarán, Soraya; Cavazos, Manuel Enrique de la O; Villarreal-Pérez, Jesús Zacarías; Gómez-Govea, Mayra A.; Garza‐González, Elvira

doi:10.1017/s0950268818002303

Cited by 6 publications

(4 citation statements)

References 28 publications

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“…The presence of different allelic profiles of recent circulating strains in Iran compared to the vaccine strains has been reported by Pertussis Reference Laboratory of Pasteur Institute of Iran [19,17,20]. The predominant antigenic profile among the isolated strains has been ptxP3, ptxA1, prn2, fim2-1 and fim3-2 which were similar to the other studies all over the world like The Netherlands, Tunisia and Mexico [6,21,7,11]. However, genomic pattern analysis of Iranian patients' isolates by pulsed field gel electrophoresis (PFGE) has shown low heterogeneity among the isolates which indicates clonal spread of B. pertussis over the past decade.…”

Section: Introductionsupporting

confidence: 83%

Characterization of a predominant Bordetella pertussis strain isolated from Iranian patients

Nikbin

Keramati

Noofeli

et al. 2018

vacres

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Introduction: Pathogen adaptation is considered as one of the important reasons for the emergence of pertussis (whooping cough). Antigenic divergence between vaccine strains and clinical isolates of Bordetella pertussis has been occurred over the years. It is suggested that the predominant genomic profile of B. pertussis has an enough capacity to spread among the population. The aim of this study was to characterize a predominant B. pertussis strain isolated from Iranian patients during 2008-2015 period. Methods: Based on the epidemiologic results of B. pertussis circulating strains in Iran, a strain named BPIP91 with predominant genomic and virulence pattern was selected from Biobank of Pasteur Institute of Iran. The antibiotic susceptibility testing was done and the growth rate of this strain was analyzed. The lethal (LD 50) and safety dose of infection of BPIP91 was also determined via mice intranasal infection. Results: Our results showed that BPIP91 was susceptible to erythromycin, azithromycin, clarithromycin, chloramphenicol, trimethoprim-sulfamethoxazole and rifampin antibiotics. The growth rate of BPIP91 was almost twofold lower than the vaccine strain. In addition, the LD 50 and infectious dose of BPIP91 strain were about 2 × 10 10 and 4 × 10 6 colony forming units, respectively. Conclusion: In this study we obtained the growth curve, LD 50 and intranasal infectious dose of a circulating strain with predominant genomic pattern in Iran. However, further examinations including determination of immunogenicity of this native strain in animal model is needed in order to evaluate its use as a vaccine strain candidate.

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Section: Introductionsupporting

confidence: 83%

Characterization of a predominant Bordetella pertussis strain isolated from Iranian patients

Nikbin

Keramati

Noofeli

et al. 2018

vacres

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“…Poland is the country with highest vaccination coverage, where WCV have been majorly used and completely shifted from ptxA 2 to ptxA 1 and ptxP1-ptxP3, prn1-prn2 and fim2-1 to fim2-2; alleles were reported in clinical isolates [ 44 , 45 ]. The wP vaccine has been used in Mexico for more than 50 years, where the appearance of ptxP3 strains started initially between 2006 and 2014 [ 46 ]. The predominant profile of circulating strains from Cambodia was ptxP3/ ptxA 1/prn2/ fim3 -1, where WCV was introduced in 1986 [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

Characterization of Bordetella pertussis Strains Isolated from India

et al. 2022

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Despite high level vaccination and the availability of two different types of vaccines, whole cell (wP) and acellular vaccines (aP), the resurgence of pertussis has been reported in many countries. Antigenic variation within circulating and vaccine strains is the most documented reason reported for the resurgence of pertussis. Research on genetic divergence among circulating and vaccine strains has largely been reported in countries using aP vaccines. There are inadequate data available for antigenic variation in B. pertussis from wP-using countries. India has used wP for more than 40 years in their primary immunization program. The present study reports five clinical isolates of B. pertussis from samples of pediatric patients with pertussis symptoms observed in India. Genotypic and phenotypic characterization of clinical isolates were performed by serotyping, genotyping, whole genome analyses and comparative genomics. All clinical isolates showed serotype 1, 2 and 3 based on the presence of fimbriae 2 and 3. Genotyping showed genetic similarities in allele types for five aP genes within vaccine strains and clinical isolates reported from India. The presence of the ptxP3 genotype was observed in two out of five clinical isolates. Whole-genome sequencing was performed for clinical isolates using the hybrid strategy of combining Illumina (short reads) and oxford nanopore (long reads) sequencing strategies. Clinical isolates (n = 5) and vaccine strains (n = 7) genomes of B. pertussis from India were compared with 744 B. pertussis closed genomes available in the public databases. The phylogenomic comparison of B. pertussis genomes reported from India will be advantageous in better understanding pertussis resurgence reported globally with respect to pathogen adaptation.

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“…The Secretariat of Health of Nuevo León, Mexico, has an extensive collection of 352 B. pertussis isolates recovered between 2007 and 2014. We previously characterized 113 of the isolates using pulsed-field gel electrophoresis (PFGE) and identified one major clone (A1) and three closely related clones ( 14 ). In this study, we performed whole-genome sequencing of four representative clones of B. pertussis circulating in the north of Mexico ( Table 1 ).…”

Section: Announcementmentioning

confidence: 99%

Genome Sequences of Bordetella pertussis Isolates from Outbreaks in Northeastern Mexico

Rodríguez-Medina

Gutiérrez-Ferman

Villarreal-Treviño

et al. 2023

Microbiol Resour Announc

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Here, we report the draft genome sequences of 4 Bordetella pertussis isolates which correspond to major clones isolated between 2008 and 2014 from two outbreaks in northeastern Mexico. The B. pertussis clinical isolates belong to the ptxP3 lineage, and they are grouped into two major clusters, defined by the fimH allele.

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Emerging ofptxP3 lineage inBordetella pertussis strains circulating in a population in northeastern Mexico

Cited by 6 publications

References 28 publications

Characterization of a predominant Bordetella pertussis strain isolated from Iranian patients

Characterization of a predominant Bordetella pertussis strain isolated from Iranian patients

Characterization of Bordetella pertussis Strains Isolated from India

Genome Sequences of Bordetella pertussis Isolates from Outbreaks in Northeastern Mexico

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