Emerging photothermal-derived multimodal synergistic therapy in combating bacterial infections

Huo, Jianhua; Jia, Qingyan; Huang, Han; Zhang, Jing; Li, Peng; Dong, Xiaochen; Huang, Wei

doi:10.1039/d1cs00074h

Cited by 500 publications

(318 citation statements)

References 235 publications

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“…ROS generation is an important factor in SACT-induced cell cytotoxicity, as shown in the previous studies. 48 It has been reported that ROS can oxidize cell membranes, causing damage to DNA, proteins, and lipids. 49 We explored the impacts of free LVFX/encapsulated LVFX on ROS generation under ultrasound stimulation.…”

Section: Discussionmentioning

confidence: 99%

Levofloxacin-Loaded Nanosonosensitizer as a Highly Efficient Therapy for Bacillus Calmette-Guérin Infections Based on Bacteria-Specific Labeling and Sonotheranostic Strategy

Jian-hu

Hou

et al. 2021

IJN

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Purpose The rapid emergence of multidrug-resistant Mycobacterium tuberculosis ( MTB ) poses a significant challenge to the treatment of tuberculosis (TB). Sonodynamic antibacterial chemotherapy (SACT) combined with sonosensitizer-loaded nanoparticles with targeted therapeutic function is highly expected to eliminate bacteria without fear of drug resistance. This study aimed to investigate the antibacterial effect and underlying mechanism of levofloxacin-loaded nanosonosensitizer with targeted therapeutic function against Bacillus Calmette-Guérin bacteria ( BCG , an MTB model). Methods This study developed levofloxacin-loaded PLGA-PEG (poly lactide-co-glycolide-polyethylene glycol) nanoparticles with BM2 aptamer conjugation on its surface using the crosslinking agents EDC and NHS (BM2-LVFX-NPs). The average diameter, zeta potential, morphology, drug-loading properties, and drug release efficiency of the BM2-LVFX-NPs were investigated. In addition, the targeting and toxicity of BM2-LVFX-NPs in the subcutaneous BCG infection model were evaluated. The biosafety, reactive oxygen species (ROS) production, cellular phagocytic effect, and antibacterial effect of BM2-LVFX-NPs in the presence of ultrasound stimulations (42 kHz, 0.67 W/cm 2 , 5 min) were also systematically evaluated. Results BM2-LVFX-NPs not only specifically recognized BCG bacteria in vitro but also gathered accurately in the lesion tissues. Drugs loaded in BM2-LVFX-NPs with the ultrasound-responsive feature were effectively released compared to the natural state. In addition, BM2-LVFX-NPs exhibited significant SACT efficiency with higher ROS production levels than others, resulting in the effective elimination of bacteria in vitro. Meanwhile, in vivo experiments, compared with other options, BM2-LVFX-NPs also exhibited an excellent therapeutic effect in a rat model with BCG infection after exposure to ultrasound. Conclusion Our work demonstrated that a nanosonosensitizer formulation with LVFX could efficiently translocate therapeutic drugs into the cell and improve the bactericidal effects under ultrasound, which could be a promising strategy for targeted therapy for MTB infections with high biosafety.

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Section: Discussionmentioning

confidence: 99%

Levofloxacin-Loaded Nanosonosensitizer as a Highly Efficient Therapy for Bacillus Calmette-Guérin Infections Based on Bacteria-Specific Labeling and Sonotheranostic Strategy

Jian-hu

Hou

et al. 2021

IJN

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“…C3 and C4 nanocomposites eliminated the totality of C. albicans. Therefore, all nanocomposites can eliminate some of the microorganisms used in this study, with C2 being the best, due to its ability to eliminate the three types of microorganisms [113][114][115]. The samples without hydrogel C3 and C4 only presented a complete response in C. albicans, and it could be considered that the photothermic effect allowed microbial elimination, based on the results shown in figure 11c, C3+light and C4+light samples [116].…”

Section: Antimicrobial Effect Of Magnetic Nanocompositesmentioning

confidence: 96%

Broad-spectrum Antimicrobial ZnMintPc Encapsulated in Magnetic-nanocomposites with Graphene Oxide/MWCNTs Based on Bimodal Action of Photodynamic and Photothermal Effect

Cuadrado¹,

Díaz-Barrios²,

Campana³

et al. 2022

Preprint

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Microbial diseases have been declared one of the main threats to humanity, which is why, in recent years, great interest has been generated in the development of nanocomposites with antimicrobial capacity. In the present work, two magnetic nanocomposites, based on Graphene Oxide (GO) and Multiwall Carbon Nanotubes (MWCNTs) were studied. The synthesis of these magnetic nanocomposites consisted of three phases: first, the synthesis of Iron Magnetic Nanoparticles (MNPs) was carried out in the presence of MWCNTs and GO using the Co-precipitation method. The second phase consisted of the adsorption of photosensitizer menthol-Zinc phthalocyanine (ZnMintPc) into MWCNTs and GO, and the third phase was the encapsulation in poly (N-vinylcaprolactam-co-poly(ethylene glycol diacrylate)) poly (VCL-co-PEGDA) polymer VCL/PEGDA a biocompatible hydrogel, in order to obtain the magnetic nanocomposites: VCL/PEGDA-MNPs-MWCNTs-ZnMintPc and VCL/PEGDA-MNPs-GO-ZnMintPc. In vitro studies were carried out using Escherichia coli and Staphylococcus aureus bacteria and the Candida albicans yeast based on the PTT/PDT effect. This research describes the optical, morphological, magnetic and photophysical characterizations of nanocomposites and their application as antimicrobial agents. It was evaluated the antimicrobial effect of magnetics nanocomposites based on the Photodynamic/Photothermal (PDT/PTT) effect; for this purpose, doses of 65 mW cm-2 at 630 nm of light were used. The VCL/PEGDA-MNPs-GO-ZnMintPc nanocomposite was able to eliminate colonies of E. coli and S. aureus, while VCL/PEGDA-MNPs-MWCNTs-ZnMintPc nanocomposite was able to eliminate the three types of microorganisms; consequently, the latter is considered a broad-spectrum of antimicrobial agent in PDT and PTT.

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“…Copyright 2019, ACS Publications antibacterial treatments. The photothermal and magnetocaloric effects of nanozymes can not only decompose biofilms by generating heat to cause thermal damage to bacteria, but also accelerate the release of nanozymes and the generated ROS and enhance the antibacterial activity of nanozymes (Huo et al, 2021;). An antibacterial nanoplatform constructed by encapsulating tungsten sulfide quantum dots (WS 2 QDs) and the antibiotic vancomycin in a thermal-sensitive liposome also showed excellent therapeutic effects on wounds infected by S. aureus due to photothermal therapy, chemodynamic therapy by free radicals and the pharmacological activity of vancomycin (Figure 2a; Xu et al, 2020) In other research, amorphous iron nanoparticles (AIronNPs) with POD-like activity were found to be influenced by an alternating magnetic field, which strengthened the catalytic activity and antibacterial effects of AIronNPs (Figure 2b; Gao et al, 2020).…”

Section: Superficial Woundsmentioning

confidence: 99%

Catalytic antimicrobial therapy using nanozymes

Wang

Jiang

Gao

2021

WIREs Nanomed Nanobiotechnol

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Nanozymes are nanomaterials with enzyme-like characteristics, which catalyze the conversion of enzyme substrates and follow enzymatic kinetics under physiological conditions. As a new generation of artificial enzymes, nanozymes provide alternative approaches for those upon enzymatic catalysis. Compared with natural enzymes, nanozymes have the advantages of simple preparation, good stability and low cost, which makes nanozymes promising for application in many fields, such as antimicrobial infection treatment. Many studies have reported that nanozymes are capable of killing a number of pathogenic bacteria with resistance, fungi as well as viruses, and have shown great curative effects for diseases caused by these pathogens. Herein, we summarize the application of nanozymes for antibacterial, antiviral, and antifungal therapies and outline the issues needing resolution in the future.

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Emerging photothermal-derived multimodal synergistic therapy in combating bacterial infections

Abstract: Photothermal therapy (PTT)-derived multimodal synergistic treatments exhibit a super-additive effect in fighting bacterial infections.

Cited by 500 publications

References 235 publications

Levofloxacin-Loaded Nanosonosensitizer as a Highly Efficient Therapy for Bacillus Calmette-Guérin Infections Based on Bacteria-Specific Labeling and Sonotheranostic Strategy

Levofloxacin-Loaded Nanosonosensitizer as a Highly Efficient Therapy for Bacillus Calmette-Guérin Infections Based on Bacteria-Specific Labeling and Sonotheranostic Strategy

Broad-spectrum Antimicrobial ZnMintPc Encapsulated in Magnetic-nanocomposites with Graphene Oxide/MWCNTs Based on Bimodal Action of Photodynamic and Photothermal Effect

Catalytic antimicrobial therapy using nanozymes

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