Emerging Roles of MicroRNA in the Embryo–Endometrium Cross Talk

Dior, Uri; Kogan, Liron; Chill, Henry H.; Eizenberg, N.; Simon, Alexander; Revel, Ariel

doi:10.1055/s-0034-1376359

Cited by 21 publications

(9 citation statements)

References 95 publications

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“…Several investigations have profiled miRNA expression in secretory and proliferative endometrium ( Kuokkanen et al 2010 , Wessels et al 2013 , Dior et al 2014 ) and differences in miRNA expression in spontaneous decidualized endometrium and early pregnancy decidua ( Wang et al 2016 ). In some studies, the endometrium was considered as a whole tissue ( Ohlsson Teague et al 2009 ), whereas in others, endometrial epithelial cells were targeted.…”

Section: Discussionmentioning

confidence: 99%

ESTROGEN-REGULATED miRNA-27b IS ALTERED BY BISPHENOL A IN ENDOMETRIAL STROMAL CELLS

Reed

Babayev²,

Chen

et al. 2018

Reproduction

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MicroRNAs (miRs) are small molecules important for regulation of transcription and translation. The objective was to identify hormonally regulated miRs in human endometrial stromal cells and to determine the impact of the endocrine disruptor, bisphenol A (BPA), on those miRs. miR microarray analysis and multiple confirmatory cell preparations treated with 17β-estradiol (E2) and BPA altered miR-27b, let-7c, let-7e and miR-181b. Further, decidualization downregulated miR-27b. VEGFB and VEGFC were validated as targets of miR-27b. Identification of miR-27b target genes suggests that BPA and E2 downregulate miR-27b thereby leading to upregulation of genes important for vascularization and angiogenesis of the endometrium during the menstrual cycle and decidualization.

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Section: Discussionmentioning

confidence: 99%

ESTROGEN-REGULATED miRNA-27b IS ALTERED BY BISPHENOL A IN ENDOMETRIAL STROMAL CELLS

Reed

Babayev²,

Chen

et al. 2018

Reproduction

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“…Studies of the miRs in the endometrium are limited to expression studies, comparing the expression of miRs in receptive with non-receptive phase endometrium ( Altmae et al, 2013 , Kresowik et al, 2014 , Kuokkanen et al, 2010 ), or in endometrium from fertile, infertile and repeat implantation failure (RIF) women ( Dior et al, 2014 , Revel et al, 2011 , Zhao et al, 2012 ), with the aim of identifying endometrial receptivity biomarkers. To date only one recent study, has investigated a functional role of miRs in human endometrial cells in vitro, specifically miR-145.…”

Section: Discussionmentioning

confidence: 99%

“…Analysis of human endometrium and trophectoderm has identified the expression of a large number of miRs ( Dior et al, 2014 , Galliano and Pellicer, 2014 , Kresowik et al, 2014 , Rosenbluth et al, 2013 ), with more recent studies demonstrating that miRs are secreted by human and bovine embryos in culture ( Kropp et al, 2014 , Rosenbluth et al, 2014 ). We hypothesised that miRs are released by human blastocysts and are taken up by endometrial surface epithelial cells to regulated endometrial receptivity and implantation.…”

Section: Introductionmentioning

confidence: 99%

Human Blastocyst Secreted microRNA Regulate Endometrial Epithelial Cell Adhesion

Cuman¹,

Sinderen²,

Gantier³

et al. 2015

EBioMedicine

122

134

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Successful embryo implantation requires synchronous development and communication between the blastocyst and the endometrium, however the mechanisms of communication in humans are virtually unknown. Recent studies have revealed that microRNAs (miRs) are present in bodily fluids and secreted by cells in culture. We have identified that human blastocysts differentially secrete miRs in a pattern associated with their implantation outcome. miR-661 was the most highly expressed miR in blastocyst culture media (BCM) from blastocysts that failed to implant (non-implanted) compared to blastocysts that implanted (implanted). Our results indicate a possible role for Argonaute 1 in the transport of miR-661 in non-implanted BCM and taken up by primary human endometrial epithelial cells (HEECs). miR-661 uptake by HEEC reduced trophoblast cell line spheroid attachment to HEEC via PVRL1. Our results suggest that human blastocysts alter the endometrial epithelial adhesion, the initiating event of implantation, via the secretion of miR, abnormalities in which result in implantation failure.

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“…miR are short, 20–24 nucleotide, non-protein coding lengths of RNA involved in post-transcriptional gene regulation 15 . miR act by binding to the 3′ untranslated region (UTR) of target genes to induce degradation of mRNA transcripts or translational repression of the protein product 16 .…”

Section: Introductionmentioning

confidence: 99%

miR-29c overexpression and COL4A1 downregulation in infertile human endometrium reduces endometrial epithelial cell adhesive capacity in vitro implying roles in receptivity

Griffiths

Sinderen

Rainczuk

et al. 2019

Sci Rep

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The endometrium is a highly complex tissue that is vulnerable to subtle gene expression changes and is the first point of contact for an implanting blastocyst. Successful blastocyst implantation can only occur when the endometrium is receptive during a short window with each menstrual cycle. microRNAs are small, non-coding RNAs that negatively regulate their gene targets. miR-29c has previously been identified to be differentially regulated across the fertile menstrual cycle, however it has not been investigated in association with infertility. We hypothesised that miR-29c dysregulation in the infertile endometrium would negatively influence endometrial adhesion and blastocyst implantation outcomes during the mid-secretory, receptive phase. miR-29c expression was elevated in early and mid-secretory phase infertile endometrium and localised to the epithelial compartments of endometrial tissue. Overexpression of miR-29c in vitro impaired endometrial epithelial adhesion, and reduced collagen type IV alpha 1 (COL4A1) mRNA expression. COL4A1 was immunolocalised to the luminal and glandular epithelial basement membranes in early and mid-secretory phase fertile and infertile endometrium for the first time. Knockdown of COL4A1 impaired endometrial epithelial adhesion suggesting a role in endometrial receptivity and implantation. Our data suggests miR-29c overexpression with infertility may impair the adhesive capacity of the endometrium, potentially contributing to implantation failure and infertility.

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Emerging Roles of MicroRNA in the Embryo–Endometrium Cross Talk

Cited by 21 publications

References 95 publications

ESTROGEN-REGULATED miRNA-27b IS ALTERED BY BISPHENOL A IN ENDOMETRIAL STROMAL CELLS

ESTROGEN-REGULATED miRNA-27b IS ALTERED BY BISPHENOL A IN ENDOMETRIAL STROMAL CELLS

Human Blastocyst Secreted microRNA Regulate Endometrial Epithelial Cell Adhesion

miR-29c overexpression and COL4A1 downregulation in infertile human endometrium reduces endometrial epithelial cell adhesive capacity in vitro implying roles in receptivity

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