Emerging technological bases for retrospective dosimetry

Straume, T.; Anspaugh, L.R.; Lucas, Joe N.; Marchetti, A. A.; Haskell, E.H.; Likhtarev, I.A.; Chumak, V.; Romanyukha, Alexander; Khrouch, Valeryi; Gavrilin, Y. U. I.; Minenko, Victor

doi:10.1002/stem.5530150726

Cited by 12 publications

(4 citation statements)

References 44 publications

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“…Some early papers described application of biodosimetric methods, including EPR dosimetry with tooth enamel, in dose reconstruction in the Chernobyl accident (Baranov et al, 1995;Vorobiev, 1997 ;Straume et al, 1997). Table 18.2 lists also some other publications reporting dose reconstructions using fewer teeth from the Chernobyl region Gualtieri et al, 2001).…”

Section: The Chernobyl Accidentmentioning

confidence: 99%

“…However, most of these reviews either covered only selected aspects of the method (Pass, 1997;Bhat, 2005), or were limited in size (Desrosiers and Schauer, 2001;Romanyukha and Regulla, 1996;Regulla, 2005;Romanyukha et al, 2000a), or were focused on the comparison of EPR dosimetry with other dosimetric techniques (Straume et al, 1997;Kleinerman et al, 2006). In 2002, an extensive report prepared by a group of authors was published by IAEA (IAEA, 2002).…”

Section: Introductionmentioning

confidence: 99%

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EPR dosimetry with tooth enamel: A review

Fattibene

Callens

2010

Applied Radiation and Isotopes

212

165

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When tooth enamel is exposed to ionizing radiation, radicals are formed that can be detected using Electron Paramagnetic Resonance (EPR) techniques. EPR dosimetry using tooth enamel is based on the (presumed) correlation between the intensity or amplitude of some of the radiation-induced signals with the dose absorbed in the enamel. In the present paper a critical review is given of this widely applied dosimetric method.The first part of the paper is quite fundamental and deals with the main properties of tooth enamel and several of its model systems (e.g., synthetic apatites). Considerable attention is also devoted to the numerous radiation-induced and native EPR signals, and the radicals responsible for them. The relevant EPR detection, identification and spectrum analysing methods are reviewed from a general point of view. Finally, the need for solid state modelling and the linearity of the dose response are investigated.The second part is devoted to the practical implementation of EPR dosimetry using enamel. It concerns the preparation of samples, specific irradiation problems, spectrum acquisition and it is described how the dosimetric signal intensity and dose can be retrieved from the EPR spectra. Special attention is paid to the energy dependence of the EPR response and to sources of uncertainties. Results of and problems encountered in international intercomparisons and epidemiological studies are also dealt with.In the final chapter the future of EPR dosimetry with tooth enamel is analysed.

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Section: The Chernobyl Accidentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

EPR dosimetry with tooth enamel: A review

Fattibene

Callens

2010

Applied Radiation and Isotopes

212

165

View full text Add to dashboard Cite

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“…Similar to dicentric chromosome formation, translocation events are proportional to the radiation dose and they persist much longer than dicentric chromosomes in peripheral lymphocytes. Chromosome translocations, particularly reciprocal translocations, can be used for retrospective biodosimetry owing to their long time persistence after IR exposure [65][66][67][68][69]. Clinical laboratories routinely use the Giemsa banding technique to analyze chromosome translocations but this technique requires substantial skill and expertise.…”

Section: Fish Based Detection Of Chromosome Translocations For Retrosmentioning

confidence: 99%

Applications of Fluorescence in Situ Hybridization in Radiation Cytogenetic Biodosimetry and Population Monitoring

Balajee¹

2018

obm genet

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The technique of in situ hybridization (ISH) using radioactively labeled DNA probes was first described in the late 1960s and early 1970s. The first use of fluorescence in situ hybridization (FISH) was reported in 1980s where RNA labeled with a fluorophore at the 3' end was used to detect specific DNA sequences. Since then, the technique has undergone various modifications for detecting single genes, chromosomes and whole genomes on various targets such as interphase nucleus, prematurely condensed chromosomes, metaphase chromosomes, fresh and paraffinized tissue sections. Although FISH is quite frequently used in clinical diagnostics, its use has recently been extended to the field of radiation biodosimetry where both stable (translocations) and unstable aberrations (dicentrics and rings) are detected with high resolution for estimating the absorbed radiation dose in humans after incidental, accidental or occupational exposure to ionizing radiation. This review summarizes the diverse applications of FISH in radiation biodosimetry that range from radiation dose estimation to prediction of deterministic (i.e. acute radiation syndrome severity) and stochastic (i.e. genetic mutations and cancer) effects in the affected human population.

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“…Translocation is yet another type of chromosome aberration induced by IR and involves the exchange of chromosome fragments between two or more chromosomes. Utility of balanced translocations involving the reciprocal exchange between the terminal portions of two chromosomes was proposed for retrospective biodosimetry due to their prolonged persistence in peripheral blood after radiation exposure [11][12][13][14][15][16]. Several studies have been published on retrospective biodosimetry using the FISH (fluorescence in situ hybridization) methodology on radiation-exposed victims of Chernobyl [4,[17][18][19] and Goiania [10,20,21].…”

Section: Introductionmentioning

confidence: 99%

Cytogenetic follow-up studies on humans with internal and external exposure to ionizing radiation

Balajee¹,

Livingston²,

Escalona³

et al. 2021

J. Radiol. Prot.

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Cells exposed to ionizing radiation have a wide spectrum of DNA lesions that include DNA single-strand breaks, DNA double-strand breaks (DSBs), oxidative base damage and DNA-protein crosslinks. Among them, DSB is the most critical lesion, which when mis-repaired leads to unstable and stable chromosome aberrations. Currently, chromosome aberration analysis is the preferred method for biological monitoring of radiation-exposed humans. Stable chromosome aberrations, such as inversions and balanced translocations, persist in the peripheral blood lymphocytes of radiation-exposed humans for several years and, therefore, are potentially useful tools to prognosticate the health risks of radiation exposure, particularly in the hematopoietic system. In this review, we summarize the cytogenetic follow-up studies performed by REAC/TS (Radiation Emergency Assistance Center/Training site, Oak Ridge, USA) on humans exposed to internal and external radiation. In the light of our observations as well as the data existing in the literature, this review attempts to highlight the importance of follow-up studies for predicting the extent of genomic instability and its impact on delayed health risks in radiation-exposed victims.

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Emerging technological bases for retrospective dosimetry

Cited by 12 publications

References 44 publications

EPR dosimetry with tooth enamel: A review

EPR dosimetry with tooth enamel: A review

Applications of Fluorescence in Situ Hybridization in Radiation Cytogenetic Biodosimetry and Population Monitoring

Cytogenetic follow-up studies on humans with internal and external exposure to ionizing radiation

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