Emerging Technologies and Fourth Generation Issues in Cartilage Repair

Kessler, Michael W.; Ackerman, George; Dines, Joshua S.; Grande, Daniel

doi:10.1097/jsa.0b013e31818d56b3

Cited by 70 publications

(46 citation statements)

References 100 publications

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“…Moreover, ADMSCs show chondrogenic potential, with the ability to maintain the differentiated chondrocyte phenotype for a long time both in vivo and in vitro (Erickson et al, 2002), and exhibit a strong capacity to form chondrocytes (Kessler et al, 2008). In the current study, the number of chondrocytes in the control and vector groups was lower than that in the hBMP-7 group, suggesting that the differentiation of ADMSCs induced by BMP-7 toward chondrocytes was effective.…”

Section: Discussionmentioning

confidence: 99%

hBMP-7 induces the differentiation of adipose-derived mesenchymal stem cells into osteoblast-like cells

et al. 2016

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ABSTRACT. The aim of this study was to investigate the differentiation potential of adipose-derived mesenchymal stem cells (ADMSCs) into osteoblasts by human bone morphogenetic protein-7 (hBMP-7) induction. ADMSCs were isolated from the subcutaneous adipose tissue of a rabbit, and then transfected with the pcDNA3.1 vector alone and pcDNA3.1-hBMP-7 (hBMP-7), respectively. Untransfected ADMSCs were used as the control group. After transfection, the morphology and green fluorescent protein (GFP) fluorescence intensity of ADMSCs were observed by fluorescent microscopy. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed to detect the growth of ADMSCs at 1, 3, and 5 days, respectively. Transmission electron microscopy was performed to observe the ultrastructural morphology of ADMSCs. In addition, ADMSCs were stained with quinalizarin and toluidine blue to reflect the content of osteoblasts and chondrocytes, respectively. Finally, the expression of collagen I and osteocalcin in ADMSCs was detected by western blot. ADMSCs were successfully isolated. Obvious GFP fluorescence and high expression of hBMP-7 demonstrated the successful transfection of hBMP-7. Specific morphological characters with a metabolically active ultrastructure were exhibited on the ADMSCs transfected with hBMP-7. In addition, the growth rate of ADMSCs transfected with hBMP-7 was significantly higher than that of the cells in the vector and control groups. Successfully induced osteoblast-like cells were identified by an obvious erythrine area and high expression of collagen I and osteocalcin in ADMSCs transfected with hBMP-7. Thus, ADMSCs can be successfully differentiated into osteoblast-like cells by hBMP-7 induction in vitro.

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Section: Discussionmentioning

confidence: 99%

hBMP-7 induces the differentiation of adipose-derived mesenchymal stem cells into osteoblast-like cells

et al. 2016

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“…17,18 Hydrogels have a high water content that creates a protective environment which mimics native cartilage. 19 They allow the addition of growth factors and cell signaling molecules that can diffuse freely. 20 The addition of bone morphogenetic peptide-7 (BMP-7) has been shown to improve the repair histology when used in addition to microfracture.…”

Section: Microfracturementioning

confidence: 99%

One-stage vs two-stage cartilage repair: a current review

Meyerkort¹,

Wood²,

Zheng³

2010

ORR

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Introduction: Articular cartilage has a poor capacity for regeneration if damaged. Various methods have been used to restore the articular surface, improve pain, function, and slow progression to osteoarthritis. Method: A PubMed review was performed on 18 March, 2010. Search terms included "autologous chondrocyte implantation (ACI)" and "microfracture" or "mosaicplasty". The aim of this review was to determine if 1-stage or 2-stage procedures for cartilage repair produced different functional outcomes. Results: The main procedures currently used are ACI and microfracture. Both first-generation ACI and microfracture result in clinical and functional improvement with no significant differences. A significant increase in functional outcome has been observed in second-generation procedures such as Hyalograft C, matrix-induced ACI, and ChondroCelect compared with microfracture. ACI results in a higher percentage of patients with clinical improvement than mosaicplasty; however, these results may take longer to achieve. Conclusion: Clinical and functional improvements have been demonstrated with ACI, microfracture, mosaicplasty, and synthetic cartilage constructs. Heterogeneous products and lack of good-quality randomized-control trials make product comparison difficult. Future developments involve scaffolds, gene therapy, growth factors, and stem cells to create a single-stage procedure that results in hyaline articular cartilage.

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“…These findings clearly suggest a correlation between a change of biomechanical properties following injury and the development of OA; however, the exact mechanisms are yet to be elucidated. OA is a chronic disease that leads to slow degradation of articular cartilage and pathological changes to the synovium as well as other connective tissues in the joint [135]. Symptoms attributed to OA involve debilitating pain in affected joints and impaired mobility [136].…”

Section: Osteoarthritismentioning

confidence: 99%

“…With the advancement of OA, pro-inflammatory cytokines including interleukin-1 and -6 (IL-1, IL-6), as well as tumour necrosis factor-α (TNF-α) cause an up-regulation of catabolic pathways in chondrocytes [135]. These cytokines play a key role in OA through activation of matrix-degrading aggrecanases ("A Disintegrin and Metalloproteinase with Thrombospondin motifs", or ADAMTS) and MMPs, as well as nitric oxide signalling pathways [141].…”

Section: Osteoarthritismentioning

confidence: 99%

“…Hydrogel materials include a wide variety of natural and synthetic polymers and allow the incorporation of bioactive molecules such as collagen [169] or HA [170] in order to stimulate chondrogenic redifferentiation [171]. Besides synthetic polymers such as poly (ethylene) glycol diacrylate (PEG) or poly(lactide-co-glycolide), natural hydrogels like agarose, alginate, or gelatin constitute a well-established material platform for cartilage models or tissue engineering purposes [135,[172][173][174]. Similar to the native cartilage ECM, the interconnected network of these hydrogels attracts and retains water, allowing nutrients and waste-products to diffuse through the constructs.…”

Section: Engineeringmentioning

confidence: 99%

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Hydrogels and bioreactors for cartilage research and functional tissue engineering

Meinert¹

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Emerging Technologies and Fourth Generation Issues in Cartilage Repair

Cited by 70 publications

References 100 publications

hBMP-7 induces the differentiation of adipose-derived mesenchymal stem cells into osteoblast-like cells

hBMP-7 induces the differentiation of adipose-derived mesenchymal stem cells into osteoblast-like cells

One-stage vs two-stage cartilage repair: a current review

Hydrogels and bioreactors for cartilage research and functional tissue engineering

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