Emerging technologies for antibiotic susceptibility testing

Behera, Bhagaban; Vishnu, G. K. Anil; Chatterjee, Suman; Sitaramgupta, V.S.N.; Sreekumar, Niranjana; Nagabhushan, Apoorva; Rajendran, Nirmala; Prathik, B H; Pandya, Hardik J.

doi:10.1016/j.bios.2019.111552

Cited by 104 publications

(93 citation statements)

References 154 publications

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“…After the isolate is obtained, the actual AST is carried out by exposing bacteria to certain antibiotics. In this respect, all the times to result mentioned in this section (and reported as "drug test time" in Table 3) only refer to the drug testing procedure, as most of the current ASTs techniques require a pure bacterial isolate as a starting point [4,95].…”

Section: Photonic Techniques For Antimicrobial Susceptibility Testingmentioning

confidence: 99%

Nanophotonics for bacterial detection and antimicrobial susceptibility testing

2020

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Photonic biosensors are a major topic of research that continues to make exciting advances. Technology has now improved sufficiently for photonics to enter the realm of microbiology and to allow for the detection of individual bacteria. Here, we discuss the different nanophotonic modalities used in this context and highlight the opportunities they offer for studying bacteria. We critically review examples from the recent literature, starting with an overview of photonic devices for the detection of bacteria, followed by a specific analysis of photonic antimicrobial susceptibility tests. We show that the intrinsic advantage of matching the optical probed volume to that of a single, or a few, bacterial cell, affords improved sensitivity while providing additional insight into single-cell properties. We illustrate our argument by comparing traditional culture-based methods, which we term macroscopic, to microscopic free-space optics and nanoscopic guided-wave optics techniques. Particular attention is devoted to this last class by discussing structures such as photonic crystal cavities, plasmonic nanostructures and interferometric configurations. These structures and associated measurement modalities are assessed in terms of limit of detection, response time and ease of implementation. Existing challenges and issues yet to be addressed will be examined and critically discussed.

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Section: Photonic Techniques For Antimicrobial Susceptibility Testingmentioning

confidence: 99%

Nanophotonics for bacterial detection and antimicrobial susceptibility testing

2020

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“…Recent developments include nanomechanical sensors, CMOS sensors, photonic crystals, and wearable sensors, which hold great promise in improving the main drawbacks of conventional methods (Mannoor et al, 2012;Nikkhoo et al, 2016;Yen and Chiu, 2020). Several recent publications review the current advances in analytical and emerging technologies for bacteria identification and antibiotic susceptibility testing (AST) (Longo and Kasas, 2014;Li et al, 2017;Syal et al, 2017a;Leonard et al, 2018;Behera et al, 2019;Shin et al, 2019). Among the emerging technologies, nanomechanical sensors have aroused great interest due to their high integration, multiplexing capability, outstanding sensitivity, and fast response with respect to other technologies (Mutharasan, 2008;Ahmed et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Nanomechanical Sensors as a Tool for Bacteria Detection and Antibiotic Susceptibility Testing

2020

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Nanomechanical biosensors refer to a subfamily of micro-electromechanical systems (MEMS) consisting of movable suspended microstructures able to convert biological processes into measurable mechanical motion. Owing to this, nanomechanical biosensors have become a promising technology in the way to detect and manage bacterial pathogens with improved effectiveness. The precise treatment of an infection relies on its early diagnosis; however, the current standard culture-based methods for bacteria detection and antibiotic susceptibility testing involve long protocols and are labor intensive. Thanks to its high sensitivity, fast response, and high throughput capability, nanomechanical technology holds great potential for overcoming some of the limitations of conventional methods. This review aims to provide a perspective on the diverse transducer structures, working principles, and detection strategies of nanomechanical sensors for bacteria detection and antibiotic susceptibility testing. Their performance in terms of sensitivity and operation time is compared with standard methods currently used in clinical microbiology laboratories. In addition, commercial systems already developed and challenges in the way of reaching real sensing application beyond the research environment are discussed.

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“…This process can often take at least 2 days (longer for slower growing organisms such as Mycobacterium tuberculosis ( M. tuberculosis )), which can lead to an increase in resistance and even be potentially fatal in time-limited situations (e.g., sepsis). Automated systems exist based on disk diffusion and broth dilution methods where time-to-result is decreased to ≈6–12 h and throughput is significantly increased but these often require a dense bacterial suspension [ 10 , 11 , 12 ]. Current AST methods are not efficient enough for modern demands, adequate care of critically ill patients and are not compatible with rapid screening at the POC.…”

Section: Introductionmentioning

confidence: 99%

Development of a Rapid, Antimicrobial Susceptibility Test for E. coli Based on Low-Cost, Screen-Printed Electrodes

et al. 2020

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Antibiotic resistance has been cited by the World Health Organisation (WHO) as one of the greatest threats to public health. Mitigating the spread of antibiotic resistance requires a multipronged approach with possible interventions including faster diagnostic testing and enhanced antibiotic stewardship. This study employs a low-cost diagnostic sensor test to rapidly pinpoint the correct antibiotic for treatment of infection. The sensor comprises a screen-printed gold electrode, modified with an antibiotic-seeded hydrogel to monitor bacterial growth. Electrochemical growth profiles of the common microorganism, Escherichia coli (E. coli) (ATCC 25922) were measured in the presence and absence of the antibiotic streptomycin. Results show a clear distinction between the E. coli growth profiles depending on whether streptomycin is present, in a timeframe of ≈2.5 h (p < 0.05), significantly quicker than the current gold standard of culture-based antimicrobial susceptibility testing. These results demonstrate a clear pathway to a low cost, phenotypic and reproducible antibiotic susceptibility testing technology for the rapid detection of E. coli within clinically relevant concentration ranges for conditions such as urinary tract infections.

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Emerging technologies for antibiotic susceptibility testing

Cited by 104 publications

References 154 publications

Nanophotonics for bacterial detection and antimicrobial susceptibility testing

Nanophotonics for bacterial detection and antimicrobial susceptibility testing

Nanomechanical Sensors as a Tool for Bacteria Detection and Antibiotic Susceptibility Testing

Development of a Rapid, Antimicrobial Susceptibility Test for E. coli Based on Low-Cost, Screen-Printed Electrodes

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