Emerging technologies in organ preservation, tissue engineering and regenerative medicine: a blessing or curse for transplantation?

Messner, Franka; Guo, Yinan; Etra, Joanna W.; Brandacher, Gerald

doi:10.1111/tri.13432

Cited by 23 publications

(21 citation statements)

References 157 publications

(194 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The utility of liver cell types is hampered by the overarching shortage of primary material, as high quality liver tissue is ear-marked for transplantation purposes where possible, leaving less optimal samples for research use. This is compounded by recent advances, which have increased the pool of viable tissue for transplantation ( 60 , 61 ). Further difficulties in using, or even benchmarking to, primary cells include the rapid loss of function of hepatocytes and other hepatic cell types during in vitro culture, particularly in monolayer ( 58 , 62 ), as well as limited proliferation potential of HSCs, combined with loss of their quiescent state in vitro ( 63 , 64 ).…”

Section: Mainmentioning

confidence: 99%

Liver Organoids: Recent Developments, Limitations and Potential

et al. 2021

View full text Add to dashboard Cite

Liver cell types derived from induced pluripotent stem cells (iPSCs) share the potential to investigate development, toxicity, as well as genetic and infectious disease in ways currently limited by the availability of primary tissue. With the added advantage of patient specificity, which can play a role in all of these areas. Many iPSC differentiation protocols focus on 3 dimensional (3D) or organotypic differentiation, as these offer the advantage of more closely mimicking in vivo systems including; the formation of tissue like architecture and interactions/crosstalk between different cell types. Ultimately such models have the potential to be used clinically and either with or more aptly, in place of animal models. Along with the development of organotypic and micro-tissue models, there will be a need to co-develop imaging technologies to enable their visualization. A variety of liver models termed “organoids” have been reported in the literature ranging from simple spheres or cysts of a single cell type, usually hepatocytes, to those containing multiple cell types combined during the differentiation process such as hepatic stellate cells, endothelial cells, and mesenchymal cells, often leading to an improved hepatic phenotype. These allow specific functions or readouts to be examined such as drug metabolism, protein secretion or an improved phenotype, but because of their relative simplicity they lack the flexibility and general applicability of ex vivo tissue culture. In the liver field these are more often constructed rather than developed together organotypically as seen in other organoid models such as brain, kidney, lung and intestine. Having access to organotypic liver like surrogates containing multiple cell types with in vivo like interactions/architecture, would provide vastly improved models for disease, toxicity and drug development, combining disciplines such as microfluidic chip technology with organoids and ultimately paving the way to new therapies.

show abstract

Section: Mainmentioning

confidence: 99%

Liver Organoids: Recent Developments, Limitations and Potential

et al. 2021

View full text Add to dashboard Cite

show abstract

“…101 Grafts can be preserved under a variety of a perfusion temperatures; these include hypothermic (0°C-12°C), mid-thermic (13°C-24°C), sub-normothermic (25°C-34°C), and normothermic (35°C-38°C) conditions. 102,103 Studies on both small and large animal VCA models have demonstrated successful preservation of graft tissue for up to 24 h using machine perfusion. [104][105][106] In 2017, Werner et al 107 applied sub-normothermic machine perfusion to preserve human limbs for 24 h with a plasma-based perfusate.…”

Section: Machine Perfusionmentioning

confidence: 99%

Preservation solutions for attenuation of ischemia–reperfusion injury in vascularized composite allotransplantation

2021

View full text Add to dashboard Cite

Vascularized composite allotransplantation represents the final level of the reconstructive ladder, offering treatment options for severe tissue loss and functional deficiencies. Vascularized composite allotransplantation is particularly susceptible to ischemia–reperfusion injury and requires preservation techniques when subjected to extended storage times prior to transplantation. While static cold storage functions to reduce ischemic damage and is widely employed in clinical settings, there exists no consensus on the ideal preservation solution for vascularized composite allotransplantation. This review aims to highlight current clinical and experimental advances in preservation solution development and their critical role in attenuating ischemia–reperfusion injury in the context of vascularized composite allotransplantation.

show abstract

“…The de-and recellularization technique has been used to produce heart, lung, liver, kidney, and intestine. Despite of some laboratory success, the technique seems to be improved before clinical application [27].…”

Section: Future Prospects 51 Organ Bioengineering and Regenerative Mmentioning

confidence: 99%

Future Prospects of Organ Transplantation

Altinors¹

2021

Organ Donation and Transplantation

View full text Add to dashboard Cite

The gap between organ demand and supply is an universal problem in organ and tissue transplantation therapy. The gap is growing in spite of efforts spent in medical, educational, social areas and mass media support. This reality has created the need for completely new therapeutic alternatives for the management of end-stage organ disease. The present research should continue in future aiming to discover systems and devices capable of totally replacing the traditional transplantation. On the other hand, a different progress in underway in transplantation. The indication for solid organ transplantation is to save life and promote quality of life. The new developing transplantations of composite tissue, uterus and face are performed with completely different indications. Facial defects caused by various insults cause serious functional and esthetic disorders, psychological and social problems. Facial transplant surgery is accomplished to overcome such problems. Uterus transplantation is emerging as an alternative to female infertility. Transplantation of composite tissue includes different organs. The main purpose of composite tissue transplantation is to restore reduced or completely lost functions and to increase the quality of life. Nerve regeneration must occur as a consequence of transplant to regain sensory and motor functions. It appears that the future of transplantation involves developments in two main streams; invention of completely new tools for solid organ transplantation and advances in the transplantation of different organs including uterus, face and composite tissue.

show abstract

Emerging technologies in organ preservation, tissue engineering and regenerative medicine: a blessing or curse for transplantation?

Cited by 23 publications

References 157 publications

Liver Organoids: Recent Developments, Limitations and Potential

Liver Organoids: Recent Developments, Limitations and Potential

Preservation solutions for attenuation of ischemia–reperfusion injury in vascularized composite allotransplantation

Future Prospects of Organ Transplantation

Contact Info

Product

Resources

About