Emerging trends in organ-on-a-chip systems for drug screening

Wang, Yanping; Gao, Yanfeng; Pan, Yongchun; Zhou, Dongtao; Liu, Yuta; Yin, Yi; Yang, Jingjing; Wang, Yuzhen; Song, Yujun

doi:10.1016/j.apsb.2023.02.006

Cited by 40 publications

(22 citation statements)

References 211 publications

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“…MPS are commonly employed for drug screening, assessing drug efficacy and toxicity. Currently, a wide array of drugs, ranging from antipsychotics and analgesics to anticancer and senolytic drugs, have been utilized through MPS for screening tests (Wang et al, 2023).…”

Section: Application Of Mps To Drug Screeningmentioning

confidence: 99%

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Microphysiological systems for human aging research

Park,

Laskow,

Chen

et al. 2024

Aging Cell

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Recent advances in microphysiological systems (MPS), also known as organs‐on‐a‐chip (OoC), enable the recapitulation of more complex organ and tissue functions on a smaller scale in vitro. MPS therefore provide the potential to better understand human diseases and physiology. To date, numerous MPS platforms have been developed for various tissues and organs, including the heart, liver, kidney, blood vessels, muscle, and adipose tissue. However, only a few studies have explored using MPS platforms to unravel the effects of aging on human physiology and the pathogenesis of age‐related diseases. Age is one of the risk factors for many diseases, and enormous interest has been devoted to aging research. As such, a human MPS aging model could provide a more predictive tool to understand the molecular and cellular mechanisms underlying human aging and age‐related diseases. These models can also be used to evaluate preclinical drugs for age‐related diseases and translate them into clinical settings. Here, we provide a review on the application of MPS in aging research. First, we offer an overview of the molecular, cellular, and physiological changes with age in several tissues or organs. Next, we discuss previous aging models and the current state of MPS for studying human aging and age‐related conditions. Lastly, we address the limitations of current MPS and present future directions on the potential of MPS platforms for human aging research.

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Section: Application Of Mps To Drug Screeningmentioning

confidence: 99%

“…The kidney serves as a pivotal regulator of blood filtration and urine production (Rehberg, 1926). Furthermore, its role is closely intertwined with metabolic processes and drug clearance (Wang et al, 2023). Various models of kidney or tubule MPS have been put…”

Section: Application Of Mps To Drug Screeningmentioning

confidence: 99%

Microphysiological systems for human aging research

Park,

Laskow,

Chen

et al. 2024

Aging Cell

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“…A lack of ECM components and a high degree of cross-linking can be toxic for synthetic polymers cell-laden bioprinting depending upon the polymer molecular weight and concentration utilized. The control over polymer concentration and degree of cross-linking of light-sensitive biomaterials provide an opportunity for the incorporation of chemical gradients with high cell densities and tissue-mimetic vasculature, rendering PB-based bioprinting highly promising . On the contrary, the typical limitations of natural biomaterials include lot-to-lot variability and limited mechanical stability.…”

Section: Photopolymerizable Bioink Componentsmentioning

confidence: 99%

“…Three-dimensional (3D) in vitro models are being increasingly explored to provide a more clinically relevant tumor model than 2D culture . 3D platforms comprising spheroid cultures, scaffold-based models, and organ-on-a-chip , offer various advantages to overcome some of the limitations of 2D cultures. Nevertheless, these techniques continue to be limited by their inability to efficiently recreate the full complexity of the TME with respect to spatial localization, vasculature, and architectural and dynamic complexity …”

Section: Introductionmentioning

confidence: 99%

Trends in Photopolymerizable Bioinks for 3D Bioprinting of Tumor Models

Gugulothu,

Asthana,

Homer-Vanniasinkam

et al. 2023

JACS Au

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Three-dimensional (3D) bioprinting technologies involving photopolymerizable bioinks (PBs) have attracted enormous attention in recent times owing to their ability to recreate complex structures with high resolution, mechanical stability, and favorable printing conditions that are suited for encapsulating cells. 3D bioprinted tissue constructs involving PBs can offer better insights into the tumor microenvironment and offer platforms for drug screening to advance cancer research. These bioinks enable the incorporation of physiologically relevant cell densities, tissue-mimetic stiffness, and vascularized channels and biochemical gradients in the 3D tumor models, unlike conventional two-dimensional (2D) cultures or other 3D scaffold fabrication technologies. In this perspective, we present the emerging techniques of 3D bioprinting using PBs in the context of cancer research, with a specific focus on the efforts to recapitulate the complexity of the tumor microenvironment. We describe printing approaches and various PB formulations compatible with these techniques along with recent attempts to bioprint 3D tumor models for studying migration and metastasis, cell–cell interactions, cell–extracellular matrix interactions, and drug screening relevant to cancer. We discuss the limitations and identify unexplored opportunities in this field for clinical and commercial translation of these emerging technologies.

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“…In this respect, innovative and advanced in vitro models mimicking the brain's complex architecture are unique tools for investigating neuronal circuitry and function. More specifically, in vitro models allow detailed electrophysiological characterization of neuronal transmission and large-scale drug screening, which is not easily carried out in vivo [9,10]. Several different types of in vitro neuronal models mimicking the complexity of neural circuits and reflecting the complex underpinnings of neurodevelopmental disorders are under intensive investigation, and researchers are shifting from two-dimensional (2D) network models to threedimensional (3D) structures and organoids [11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Development of multi-depth probing 3D microelectrode array to record electrophysiological activity within neural cultures

Yadav,

Lisa,

Giacomozzi

et al. 2023

J. Micromech. Microeng.

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Microelectrode arrays (MEAs) play a crucial role in investigating the electrophysiological activities of neuronal populations. Although two-dimensional neuronal cell cultures have predominated in neurophysiology in monitoring in-vitro the electrophysiological activity, recent research shifted toward culture using three-dimensional (3D) neuronal network structures for developing more sophisticated and realistic neuronal models. Nevertheless, many challenges remain in the electrophysiological analysis of 3D neuron cultures, among them the development of robust platforms for investigating the electrophysiological signal at multiple depths of the 3D neurons’ networks. While various 3D MEAs have been developed to probe specific depths within the layered nervous system, the fabrication of microelectrodes with different heights, capable of probing neural activity from the surface as well as from the different layers within the neural construct, remains challenging. This study presents a novel 3D MEA with microelectrodes of different heights, realized through a multi-stage mold-assisted electrodeposition process. Our pioneering platform allows meticulous control over the height of individual microelectrodes as well as the array topology, paving the way for the fabrication of 3D MEAs consisting of electrodes with multiple heights that could be tailored for specific applications and experiments. The device performance was characterized by measuring electrochemical impedance, and noise, and capturing spontaneous electrophysiological activity from neurospheroids derived from human induced pluripotent stem cells. These evaluations unequivocally validated the significant potential of our innovative multi-height 3D MEA as an avant-garde platform for in vitro 3D neuronal studies.

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Emerging trends in organ-on-a-chip systems for drug screening

Cited by 40 publications

References 211 publications

Microphysiological systems for human aging research

Microphysiological systems for human aging research

Trends in Photopolymerizable Bioinks for 3D Bioprinting of Tumor Models

Development of multi-depth probing 3D microelectrode array to record electrophysiological activity within neural cultures

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