Emersion survival manipulation in Greenshell™ mussels (Perna canaliculus): Implications for the extension of live mussels' shelf-life

Zamora, Leonardo N.; Ragg, Norman L.C.; Hilton, Zoë; Webb, Stephen C.; King, Nathan; Adams, Serean L.

doi:10.1016/j.aquaculture.2018.10.057

Cited by 16 publications

(6 citation statements)

References 27 publications

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“…The differential ROS signal between treatments may be explained by differences in behaviour between mussels maintained under different conditions during emersion. It is possible that mussels opened their valves during emersion while others closed their valves, as observed in adult mussels showing different gaping behavioural strategies in response to emersion ( Powell et al, 2017 ; Zamora et al, 2019 ). Mussels closing their valves would be potentially under anaerobic metabolism, presumably reducing ROS production compared to mussels attempting to maintain aerobic respiration under emersion conditions.…”

Section: Resultsmentioning

confidence: 99%

A new method to localise and quantify oxidative stress in live juvenile mussels

et al. 2021

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Stress and survival of juvenile New Zealand green-lipped mussel, Perna canaliculus, is a poorly-understood bottleneck in the ecological and economic performance of a significant aquaculture crop. This species was therefore selected as a model organism for the development of a new method to quantify oxidative stress in whole individuals. An in vivo ROS-activated stain (CellROX™) was administered to anaesthetised, translucent juveniles that were subsequently formaldehyde-fixed and visualised using confocal microscopy. Subsequent application of image analysis to quantifying ROS-positive tissue areas was successfully used to detect stress differences in juvenile mussels exposed to varying levels of emersion. This integrated method can be used to localise and quantify ROS production in individual translucent bivalve life stages (larval and juvenile), while relative stability following fixation greatly expands potential practical field applications.

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Section: Resultsmentioning

confidence: 99%

A new method to localise and quantify oxidative stress in live juvenile mussels

et al. 2021

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“…During valve closure, there is a shift from aerobic to anaerobic metabolism, resulting in a decrease in free radical production, protecting the individual from oxidative damage [54]. During emersion, mussels also use gaping behaviour to decrease their body temperature and facilitate gas exchange, helping mussels cope with long emersion periods [55][56][57]. In juvenile mussels, gaping behaviour has not been studied in detail, but previous research in P. canaliculus indicates that juvenile mussels gape during long emersion [19], potentially as a mechanism to sustain aerobic metabolism.…”

Section: Discussionmentioning

confidence: 99%

Oxidative Damage and Antioxidants as Markers for the Selection of Emersion Hardening Treatments in GreenshellTM Mussel Juveniles (Perna canaliculus)

Delorme,

Burritt,

Zamora

et al. 2024

Antioxidants

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Transport out of the water is one of the most challenging events for juvenile Perna canaliculus and can be a highly inefficient process, with many juveniles subsequently being lost following extended periods of emersion. Hardening techniques offer a possible method for reducing transport-related stress. In this study, different hardening treatments (short, long and intermittent sub-lethal emersion) were used to prepare ~1.2 mm P.canaliculus for transport (20 h) and subsequent reoxygenation stress during re-immersion (i.e., recovery). The oxidative stress responses, resettlement behaviour, respiration rates and survival of the mussels after transport and during recovery were all assessed. Short emersion (1 h) as a hardening treatment prior to transport did not cause major stress to the mussels, which maintained respiration at control levels, showed significantly stimulated antioxidant defences during recovery, showed greater resettlement behaviour and remained viable after 24 h of recovery. In comparison, the long and intermittent emersion treatments negatively impacted oxidative stress responses and affected the viability of the mussels after 24 h of recovery. This study showed that exposing juvenile P.canaliculus to a mild stress prior to transport may stimulate protective mechanisms, therefore eliciting a hardening response, but care must be taken to avoid overstressing the mussels. Improving the management of stress during the transport of juvenile mussels may be key to minimising mussel losses and increasing harvest production, and biomarkers associated with oxidative stress/antioxidant metabolism could be valuable tools to ensure emersion hardening does not overstress the mussels and reduce survival.

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“…The results of this study cannot confirm whether the heat-related mortality observed in the intertidally restored mussels was a factor of their subtidal origin as there was no available population of natural intertidal mussels for comparison or reciprocal transplantation. Nevertheless, at temperatures over 40 C even intertidally reared mussels experience increased hormonal perturbations, loss of neural control, and failure of protein synthesis (Dunphy et al, 2015(Dunphy et al, , 2018Zamora et al, 2019). These effects culminate in mass mortality events for wild intertidal mussels when extreme heat waves correspond with low tides (Capelle et al, 2021;Seuront et al, 2019;Tsuchiya, 1983).…”

Section: Discussionmentioning

confidence: 99%

Out of their depth: The successful use of cultured subtidal mussels for intertidal restoration

Toone

Hillman

Benjamin

et al. 2023

Conservat Sci and Prac

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Ecosystem restoration has proliferated across the globe to combat widespread ecosystem decline. Translocations of viable individuals into degraded habitats form a core component of restoration efforts, but the selection of source populations poses challenges because phenotypic differences between source populations and depleted populations result in low post‐translocation survival. Intertidal mussel restoration is typically reliant on subtidal source populations despite their weaker shells, lighter weights, and poorer respiratory abilities compared to intertidal mussels. Intertidal mussel restoration using subtidal mussels has recorded very low survival, leading to suggestions that subtidal mussels are unfit for the intertidal zone, effectively eliminating the possibility of translocation‐based intertidal mussel restoration. In this study, small‐scale intertidal mussel restoration was tested using subtidal cultured mussels transplanted to paired plots, half in the lower intertidal and half in the upper subtidal. The results demonstrate the first scientific evidence of the successful restoration of intertidal mussel reefs using subtidal mussels at this scale. Specifically, one location recorded very high survival after 12 months (95.1 ± 0.6%; mean ± SE), comparable to the survival recorded in the subtidal zone (97.6 ± 0.3%). Translocations to the intertidal at the other two locations experienced mortality during the Austral summer (final survival: 81.3 ± 1.6% and 56.6 ± 7.4%), which directly correlated with exposure to extreme temperatures (>40°C). These results reveal that despite phenotypic differences, subtidal cultured mussels are potential sources for successful intertidal restoration. However, care should also be taken to avoid locations prone to high temperatures, particularly in light of a warming global environment. Ultimately, to achieve the massive upscaling in restoration called for by the UN Decade on Ecosystem Restoration, we encourage restoration managers to think broadly about potential source populations, avoid rejections based solely on phenotypic differences, and explore every potential avenue for restoration success.

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Emersion survival manipulation in Greenshell™ mussels (Perna canaliculus): Implications for the extension of live mussels' shelf-life

Cited by 16 publications

References 27 publications

A new method to localise and quantify oxidative stress in live juvenile mussels

A new method to localise and quantify oxidative stress in live juvenile mussels

Oxidative Damage and Antioxidants as Markers for the Selection of Emersion Hardening Treatments in GreenshellTM Mussel Juveniles (Perna canaliculus)

Out of their depth: The successful use of cultured subtidal mussels for intertidal restoration

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