Emetine resistance in Chinese hamster cells. Analysis of ribosomal proteins prepared from mutant cells.

Boersma, D; McGill, S; Mollenkamp, J W; Roufa, D J

doi:10.1016/s0021-9258(17)37952-8

Cited by 55 publications

(4 citation statements)

References 37 publications

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“…It can be seen from the table that the unstained plugs from the upper left quadrant of the gel were highly labeled and that the 3H/14C ratios of both the unstained plugs and the r proteins from this region were greater than those in the other portions of the electrophorogram. The presence of weakly basic contaminant proteins in gels of r proteins has been recently reported by Boersma et al (1979).…”

Section: Resultsmentioning

confidence: 86%

Preferential stimulation of ribosomal protein synthesis by insulin and in the absence of ribosomal and messenger ribonucleic acid formation

DePhilip¹,

Rudert²,

Lieberman³

1980

Biochemistry

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Section: Resultsmentioning

confidence: 86%

Preferential stimulation of ribosomal protein synthesis by insulin and in the absence of ribosomal and messenger ribonucleic acid formation

DePhilip¹,

Rudert²,

Lieberman³

1980

Biochemistry

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“…(id) All of the ith emetine); -, lack of comple-emetine-resistant CHO mutants tested thus far nately equal numbers of hybrids belong to the same complementation group (2, without emetine); ND, not deter-9, 10). In at least two CHO mutants the same 40S ribosomal protein has been shown to be electrophoretically altered (1,12). (iii) Campbell and Worton (4) have shown that the locus they have designated as emt in CHO cells is linked to a gene, chr, defined by mutants resistant to chromate.…”

Section: Resultsmentioning

confidence: 99%

“…In all the emetine-resistant CHO mutants which have been examined in enough detail, the lesions could be linked to the 40S ribosomal subunit (8). In two such mutants, the same 40S ribosomal protein has been shown to be electrophoretically altered (1,12). In addition, there is considerable evidence that CHO cells are haploid for one locus that can be altered to give rise to the emetine-resistant phenotype (3,6).…”

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confidence: 99%

Identification and Characterization of a Third Complementation Group of Emetine-Resistant Chinese Hamster Cell Mutants

Wasmuth

Hill

Vock

1981

Molecular and Cellular Biology

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We have isolated emetine-resistant cell lines from Chinese hamster peritoneal fibroblasts and have shown that they represent a third distinct class or complementation group of emetine-resistant mutants, as determined by three different criteria. These mutants, like those belonging to the two other complementation groups we have previously defined, which were isolated from Chinese hamster lung and Chinese hamster ovary cells, have alterations that directly affect the protein biosynthetic machinery. So far, there is absolute cell line specificity with respect to the three complementation groups, in that all the emetine-resistant mutants we have isolated from Chinese hamster lung cells belong to one complementation group, all those we have isolated from Chinese hamster ovary cells belong to a second complementation group, and all those isolated from Chinese hamster peritoneal cells belong to a third complementation group. Thus, in cultured Chinese hamster cells, mutations in at least three different loci, designated emtA, emtB, and emtC, encoding for different components of the protein biosynthetic machinery, can give rise to the emetine-resistant phenotype.

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“…The available evidence suggests that all CHO emetine-resistant mutants reported thus far belong to this same complementation group. In addition, two laboratories have identified a 40S ribosomal protein as being electrophoretically altered in independently isolated emetine-resistant CHO mutants (2,14).…”

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confidence: 99%

Linkage in cultured Chinese hamster cells of two genes, emtB and leuS, involved in protein synthesis and isolation of cell lines with mutations in three linked genes.

Wasmuth¹,

Chu²

1980

The Journal of Cell Biology

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We have determined via segregation analyses from appropriate hybrids that two genes involved in protein synthesis, one encoding for a ribosomal protein (emtB) and one encoding for leucyl-tRNA synthetase (IeuS), cosegregate at a very high frequency and are linked in both Chinese hamster ovary and lung cells. In contrast, the emtA locus, defined by a second complementation group of emetine-resistant mutants which also have alterations affecting protein synthesis and probably the ribosome, is not linked to IeuS . In addition, we have determined that a third gene, one that can be altered to give rise to chromate resistance, is syntenic with emtB and IeuS . We have selected cell lines with mutations in each of these three linked genes and have shown that the three loci cosegregate at a high frequency. Because the mutations in these three linked genes provide easily distinguishable phenotypes, these cell lines should provide a powerful tool for examining several important questions concerning mitotic recombination in somatic cells.The genetic and biochemical dissection of protein synthesis in mammalian cells has benefited greatly in recent years from the use of mutant methodology. A large number of cultured somatic cell mutants have been isolated with alterations in different components of the protein synthetic machinery (9,10,12,16,18,19). One goal of this laboratory is to expand the number of such mutants in cultured Chinese hamster cells and to characterize them at the molecular level, especially mutants with alterations affecting various components of the ribosome . A second goal is to use such mutants to begin constructing a genetic map of this group of functionally related genes.We have isolated and partially characterized three distinct types of Chinese hamster cell mutants that were selected as being resistant to the protein synthesis inhibitor, emetine (19; J Hill, L. Vock, and J. Wasmuth, manuscript in preparation) . Although the phenotypes of all three types of mutants are very similar, and all have alterations affecting protein synthesis directly, they clearly define three distinct complementation groups . For all three classes, the emetine-resistant phenotypes are recessive. We have designated the loci defined by these different complementation groups as emtA, emtB, and emtC . Two laboratories (4, 8) have obtained evidence that Chinese hamster ovary (CHO), but not Chinese hamster lung (CHL), cells are functionally hemizygous for a locus that can be altered

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Emetine resistance in Chinese hamster cells. Analysis of ribosomal proteins prepared from mutant cells.

Cited by 55 publications

References 37 publications

Preferential stimulation of ribosomal protein synthesis by insulin and in the absence of ribosomal and messenger ribonucleic acid formation

Preferential stimulation of ribosomal protein synthesis by insulin and in the absence of ribosomal and messenger ribonucleic acid formation

Identification and Characterization of a Third Complementation Group of Emetine-Resistant Chinese Hamster Cell Mutants

Linkage in cultured Chinese hamster cells of two genes, emtB and leuS, involved in protein synthesis and isolation of cell lines with mutations in three linked genes.

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