Emission of ESBL/AmpC-producing Escherichia coli from pig fattening farms to surrounding areas

Salviati, C. von; Laube, H.; Guerra, Beatriz; Roesler, Uwe; Friese, Anika

doi:10.1016/j.vetmic.2014.10.010

Cited by 82 publications

(62 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…CTX-M-1 was the most prevalent beta-lactamase in isolates from flies, which is in line with an overall higher proportion of the CTX-M-1 group in livestock compared to humans (Strauß et al, 2015;von Salviati et al, 2015). In contrast, the CTX-M-9 group (incl.…”

Section: The High Proportion Of S Fonticola Among All Esbl-e Is Not supporting

confidence: 56%

See 1 more Smart Citation

A geospatial analysis of flies and the spread of antimicrobial resistant bacteria

Schaumburg

Onwugamba

Akulenko

et al. 2016

International Journal of Medical Microbiology

View full text Add to dashboard Cite

Section: The High Proportion Of S Fonticola Among All Esbl-e Is Not supporting

confidence: 56%

“…Recent studies reported ESBL-E on flies in the vicinity of livestock farms in the Netherlands and Germany (Blaak et al, 2014;von Salviati et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

A geospatial analysis of flies and the spread of antimicrobial resistant bacteria

Schaumburg

Onwugamba

Akulenko

et al. 2016

International Journal of Medical Microbiology

View full text Add to dashboard Cite

“…In contaminated stables, MRSA and ESBL-E have been detected in water (49,50), air (33,51), and dust (52,53), as well as in fodder (33,50) and liquid manure (33,51). During the previous monitoring program, microbiological analysis revealed the presence of MRSA in all samples of the stable air of the model pig farm and ESBL-E had been detected in one out of two air samples collected within the stable.…”

Section: Situation At the Outset (I) Prevalence Of Mrsa And Esbl-e Imentioning

confidence: 99%

Eradication of Methicillin-Resistant Staphylococcus aureus and of Enterobacteriaceae Expressing Extended-Spectrum Beta-Lactamases on a Model Pig Farm

Schmithausen

Kellner

Schulze-Geisthoevel

et al. 2015

Appl Environ Microbiol

View full text Add to dashboard Cite

g Colonization of livestock with bacteria resistant to antibiotics is considered a risk for the entry of drug-resistant pathogens into the food chain. For this reason, there is a need for novel concepts to address the eradication of drug-resistant commensals on farms. In the present report, we evaluated the decontamination measures taken on a farm contaminated with methicillin-resistant Staphylococcus aureus (MRSA) and Enterobacteriaceae expressing extended-spectrum ␤-lactamases (ESBL-E). The decontamination process preceded the conversion from piglet breeding to gilt production. Microbiological surveillance showed that the decontamination measures eliminated the MRSA and ESBL-E strains that were detected on the farm before the complete removal of pigs, cleaning and disinfection of the stable, and construction of an additional stable meeting high-quality standards. After pig production was restarted, ESBL-E remained undetectable over 12 months, but MRSA was recovered from pigs and the environment within the first 2 days. However, spa (Staphylococcus aureus protein A gene) typing revealed acquisition of an MRSA strain (type t034) that had not been detected before decontamination. Interestingly, we observed that a farmworker who had been colonized with the prior MRSA strain (t2011) acquired the new strain (t034) after 2 months. In summary, this report demonstrates that decontamination protocols similar to those used here can lead to successful elimination of contaminating MRSA and ESBL-E in pigs and the stable environment. Nevertheless, decontamination protocols do not prevent the acquisition of new MRSA strains. N umerous studies have highlighted the emergence of methicillin-resistant Staphylococcus aureus (MRSA) (1-4) and Enterobacteriaceae expressing extended-spectrum ␤-lactamases (ESBL-E) (5-7) in livestock production, particularly in pigs. Hence, there is an ongoing debate whether the use of antibiotics in food animal production represents an important source of continuous spread of MRSA and ESBL-E to humans (8-10). Farmers are confronted with two different consequences of this problem: the potential danger of animal colonization with drug-resistant bacteria for (i) humans living on or in the vicinity of farms (11-13) and for (ii) consumers of animal products (14-18). Indeed, livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) have been found in humans living and working in close contact with pigs and also in hospitals in rural areas (11,(19)(20)(21)(22)(23)(24)(25). In contrast, ESBL-E could possibly be transferred from animals to humans via meat products (16,(26)(27)(28). As a consequence, control points to limit transmission of resistant pathogens "from stable to table" have been demanded (29).Notably, bacteria not only persist on/in the living animal but also on surfaces that are in contact with the animals, such as barn walls and equipment (30, 31). LA-MRSA isolates were detected in dust samples from the investigated breeding farms in Germany as part of the EFSA (European Food Safe...

show abstract

“…Slaughterhouse biosecurity must be increased to prevent dissemination of ESBL producing E. coli to environment. Moreover, vectors such as mice and flies may also play a part in the spread of antimicrobial resistance in farms [17].…”

Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 99%

CTX-M producing Escherichia coli isolated from cattle feces in Bogor slaughterhouse, Indonesia

Sudarwanto

Lukman

Latif

et al. 2016

Asian Pacific Journal of Tropical Biomedicine

View full text Add to dashboard Cite

Objective: To determine the occurrence of CTX-M producing Escherichia coli (E. coli) from cattle feces in Bogor slaughterhouse, Indonesia. Methods: A total of 220 cattle feces samples were collected from Bogor slaughterhouse from March to April 2015. Presence of extended-spectrum beta-lactamase (ESBL) producing E. coli was detected by disc diffusion test based on the recommendation from Clinical and Laboratory Standards Institute (2014). Bacterial strains which were confirmed as producing ESBLs were further analyzed for the presence of bla genes of the ESBL by PCR. Results: The results showed that CTX-M producing E. coli isolates were detected in 19 samples from 220 samples (8.6%). The β-lactamase genes detected were CTX-M-1 (n = 10) and CTX-M-9 (n = 9). All of the CTX-M producing E. coli isolates showed multidrug resistance phenotypes to at least four antibiotics. The highest incidence of antibiotics resistance was showed to ampicillin (100.0%), cefotaxime (100.0%), and cefpodoxime (100.0%), followed by streptomycin (84.3%), trimethoprim-sulfamethoxazole (73.7%), erythromycin (52.6%), kanamycin (26.3%), doxycycline (10.5%), and ceftazidime (0.0%). Conclusions: Detection of CTX-M-producing E. coli in cattle feces raises important questions as they can represent a potential risk factor to public health.

show abstract

Emission of ESBL/AmpC-producing Escherichia coli from pig fattening farms to surrounding areas

Cited by 82 publications

References 31 publications

A geospatial analysis of flies and the spread of antimicrobial resistant bacteria

A geospatial analysis of flies and the spread of antimicrobial resistant bacteria

Eradication of Methicillin-Resistant Staphylococcus aureus and of Enterobacteriaceae Expressing Extended-Spectrum Beta-Lactamases on a Model Pig Farm

CTX-M producing Escherichia coli isolated from cattle feces in Bogor slaughterhouse, Indonesia

Contact Info

Product

Resources

About