Emission of Methane by Eudrilus eugeniae and Other Earthworms from Brazil

Depkat-Jakob, Peter S.; Hunger, Sindy; Schulz, Kristin; Brown, G. G.; Tsai, Siu Mui; Drake, Harold L.

doi:10.1128/aem.07949-11

Cited by 33 publications

(20 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Gut content (approximately 25 g) was squeezed out from approximately 100 earthworms while gassing with 100% argon to minimize exposure of the gut content to air (Depkat-Jakob et al, 2012). Gut content was kept under an anoxic atmosphere of 100% argon in sterile serum bottles and mixed to a 1:10 (w/v) dilution with sterile anoxic sodium phosphate buffer (Wü st et al, 2011).…”

Section: Gut Content Microcosmsmentioning

confidence: 99%

“…Nucleic acids were extracted as previously described (Depkat-Jakob et al, 2012), and the extracts of each replicate were pooled per treatment. DNA was degraded by treatment with 1 U DNAse I per ml (Fermentas GmbH, St Leon-Rot, Germany), and RNA was purified by precipitation with 0.7 volume isopropyl alcohol and 0.1 volume of 5 mM sodium chloride (Merck KGaA, Darmstadt, Germany).…”

Section: Extraction Of Nucleic Acidsmentioning

confidence: 99%

“…Extracted RNA was transformed into complementary DNA (cDNA) by reverse transcription with SuperScript III reverse transcriptase as previously described (Depkat-Jakob et al, 2012). PCR amplification of bacterial 16S rRNA genes from cDNA was performed with the primers 27F and 907RM (5 0 -AGAGTTTGATCMTGGCTC-3 0 ; 5 0 -CCGTCAATTC MTTTGAGTTT-3 0 ; Lane, 1991 Master Mix (5Prime, Hamburg, Germany), 1 mM magnesium chloride and 0.6 mM of each primer.…”

Section: Reverse Transcription and Pcr Amplificationmentioning

confidence: 99%

“…It was therefore surprising that certain earthworms in Brazil, in particular Eudrilus eugeniae, were recently discovered to emit CH 4 in vivo (Depkat-Jakob et al, 2012). E. eugeniae is an epigeic species that is native to certain African soils but is commonly used in vermicomposting in other countries, including Brazil (Martinez, 1998;Domínguez, 2004;Oboh et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…E. eugeniae is an epigeic species that is native to certain African soils but is commonly used in vermicomposting in other countries, including Brazil (Martinez, 1998;Domínguez, 2004;Oboh et al, 2007). The emission of CH 4 by E. eugeniae appeared to be linked to the ingested methanogens of the families Methanosarcinaceae, Methanobacteriaceae and Methanomicrobiaceae (Depkat-Jakob et al, 2012). We hypothesized that the capacity of E. eugeniae to emit CH 4 is linked to diverse fermentations in the alimentary canal, and the main objective of this study was to resolve anaerobic processes and associated taxa that can potentially drive methanogenesis in the gut of E. eugeniae.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Methanogenic food web in the gut contents of methane-emitting earthwormEudrilus eugeniaefrom Brazil

et al. 2015

Self Cite

View full text Add to dashboard Cite

The anoxic saccharide-rich conditions of the earthworm gut provide an ideal transient habitat for ingested microbes capable of anaerobiosis. It was recently discovered that the earthworm Eudrilus eugeniae from Brazil can emit methane (CH 4 ) and that ingested methanogens might be associated with this emission. The objective of this study was to resolve trophic interactions of bacteria and methanogens in the methanogenic food web in the gut contents of E. eugeniae. RNA-based stable isotope probing of bacterial 16S rRNA as well as mcrA and mrtA (the alpha subunit of methyl-CoM reductase and its isoenzyme, respectively) of methanogens was performed with [ 13 C]-glucose as a model saccharide in the gut contents. Concomitant fermentations were augmented by the rapid consumption of glucose, yielding numerous products, including molecular hydrogen (H 2 ), carbon dioxide (CO 2 ), formate, acetate, ethanol, lactate, succinate and propionate. Aeromonadaceaeaffiliated facultative aerobes, and obligate anaerobes affiliated to Lachnospiraceae, Veillonellaceae and Ruminococcaceae were associated with the diverse fermentations. Methanogenesis was ongoing during incubations, and 13 C-labeling of CH 4 verified that supplemental [ 13 C]-glucose derived carbon was dissimilated to CH 4 . Hydrogenotrophic methanogens affiliated with Methanobacteriaceae and Methanoregulaceae were linked to methanogenesis, and acetogens related to Peptostreptoccocaceae were likewise found to be participants in the methanogenic food web. H 2 rather than acetate stimulated methanogenesis in the methanogenic gut content enrichments, and acetogens appeared to dissimilate supplemental H 2 to acetate in methanogenic enrichments. These findings provide insight on the processes and associated taxa potentially linked to methanogenesis and the turnover of organic carbon in the alimentary canal of methane-emitting E. eugeniae.

show abstract

Section: Gut Content Microcosmsmentioning

confidence: 99%

Section: Extraction Of Nucleic Acidsmentioning

confidence: 99%

Section: Reverse Transcription and Pcr Amplificationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations