Emodin, A Naturally Occuring Anthraquinone: Its Isolation and Spectrophotometric Determination in Rumex Cyprius Plant

Al-Nuri, Mohammed; Zatar, Nidal A.; Abu-Eid, Maher; Hannoun, Mohammad; J, Al-Jondi W; Hussein, Ahmad I.; Ali‐Shtayeh, Mohammed S.

doi:10.1080/00387019608007144

Cited by 4 publications

(2 citation statements)

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“…Emodin was determined in its natural sources by several methods as thin layer chromatography (TLC) (6-8) HPLC (9) , capillary electrophoresis (10,11) and high performance liquid chromatography (HPLC) (12)(13)(14)(15). Al-Nuri etal (9) suggested a spectrophorometric method for determination of emodin by measuring the absorbance at 250 am.…”

Section: Introductionmentioning

confidence: 99%

Adsorptive Cathodic Stripping Voltammetric Studies of Emodin

Hannoun¹

1999

An-Najah University Journal for Research - A

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A differential pulse cathodic stripping voltammetric (DP-CSV) method for determination of emodin in Britton- Robinson buffer (pH2) at a hanging mercury drop electrode (HMIDE) is described. The method is based on measuring the reductive peak height at —0.25V vs. Ag/AgCl reference electrode. The linear relationship between the peak current and emodin concentration allowed the voltarnmetric determination of emodin over a wide concentration range 1.0 X 10-2 —2.5 X10-6 M, with a relative standard deviation of 3.3% (10 determinations at 1 X 10-2 M). Adsorption of emodin at HMDE enabled a detection limit of 2X10-1°M after 3 min accumulation at 0.0V. The applicability of the suggested method was found to be suitable for the determination of emodin in roots, stems and leaves of Rumex cyprius plant.

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Section: Introductionmentioning

confidence: 99%

Adsorptive Cathodic Stripping Voltammetric Studies of Emodin

Hannoun¹

1999

An-Najah University Journal for Research - A

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“…Figure 1 displays the structures of the four anthraquinones, which are typically used as cathartics and also exhibit useful antitumor activity in the related anthracycline antibiotics 27,. 28 Anthraquinones have been studied by various analytical methods, including UV/Vis spectrometry, EIMS, NCIMS, 13 C‐ and 1 H‐NMR, and high‐performance liquid chromatography 27–34. However, anthraquinones and alkyl anthraquinones have only been analyzed by EIMS in a traditional internal source ITMS.…”

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confidence: 99%

Probing reactive sites for ion‐molecule reactions of anthraquinones with dimethyl ether using an external source ion trap tandem mass spectrometer and computational chemistry

Chen

2001

Rapid Comm Mass Spectrometry

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Gas-phase ion-molecule reactions of anthraquinone derivatives with dimethyl ether (DME) were investigated using an external source ion trap mass spectrometer. Semi-empirical calculations were executed to determine possible reactive sites for the product ions. Collision activated dissociation (CAD) was successfully performed for very low abundance of ion-molecule products. Even for product ions with a relative intensity below 1%, CAD experiments can be successfully performed. Significantly more structural information could be elucidated based on this special feature. Importantly, the CAD spectra of very minor ions could be measured by this ion trap instrument, which significantly enhances the future role of the ion trap as a powerful analytical instrument. CAD of all product ions on anthraquinone compounds typically eliminates neutral molecules such as CO or H(2)O. A hydration phenomenon in the CAD processes resulting from the precursor ions incorporating one molecule of H(2)O and then eliminating one molecule of CO was observed in this study.

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Effects of increased accumulation of doxorubicin due to emodin on efflux transporter and LRP1 expression in lung adenocarcinoma and colorectal carcinoma cells

2018

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Treatment with doxorubicin (dox) and emodin, separately and together, under normoxic and hypoxia-like conditions induced by CoCl, led to greater intracellular compound accumulation over 10 h post-addition in the presence of CoCl in lung adenocarcinoma (A549) and colorectal carcinoma (HCT-15) cell lines. Confocal microscopy revealed that emodin, by itself, showed high cytosolic distribution in both cell lines, at 40 min post-addition but had entered the nuclei by 2 h, while dox entered the nuclei by 40 min. Both compounds modulated the expression of the efflux transporters (PgP, ABCG2, or MRP1-4) and the endocytic receptor, low-density lipoprotein receptor-related protein 1 (LRP1), to different extents under the study conditions. Efflux transporter upregulation was linked to lower intracellular compound levels due to greater efflux. Increased dox accumulation was accompanied by unaltered expression or upregulation of LRP1 in A549 cells. In both cell lines, increased accumulation of dox and emodin was observed whenever LRP1 and the efflux transporters known to transport dox and emodin were all up- or downregulated concomitantly. Increased growth inhibition was linked to co-treatment with dox and emodin and with increased ligand accumulation. The results presented in this study raise the hypothesis that higher production of LRP1 protein may be associated with higher endocytosis of upregulated transporter proteins at the cell surface, and hence, increased dox and emodin accumulation and growth inhibition. If so, elevation of LRP1 expression may be a useful target for interventions to promote the efficacy of these and other anticancer drugs.

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Emodin, A Naturally Occuring Anthraquinone: Its Isolation and Spectrophotometric Determination in Rumex Cyprius Plant

Cited by 4 publications

References 1 publication

Adsorptive Cathodic Stripping Voltammetric Studies of Emodin

Adsorptive Cathodic Stripping Voltammetric Studies of Emodin

Probing reactive sites for ion‐molecule reactions of anthraquinones with dimethyl ether using an external source ion trap tandem mass spectrometer and computational chemistry

Effects of increased accumulation of doxorubicin due to emodin on efflux transporter and LRP1 expression in lung adenocarcinoma and colorectal carcinoma cells

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