Emphasized Mechanistic Antimicrobial Study of Biofunctionalized Silver Nanoparticles on Model<i> Proteus mirabilis</i>

Parveen, Asra; Yalagatti, Manjunath Sooganna; Venkataraman, A.; Deshpande, Raghunandan

doi:10.1155/2018/3850139

Cited by 14 publications

(6 citation statements)

References 45 publications

(35 reference statements)

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“…This disintegration of the membrane was affirmed by increasing in the leakage of DNA and proteins. These findings concord with previous reports, suggesting that the antimicrobial effect of various nanoparticles may be attributed to the disintegration of membranes and leakage of the intracellular content, as well as the rupture of cell walls [ 2 , 86 , 87 , 88 , 89 , 90 , 91 ]. Interestingly, the biogenic TeNPs produced by Gayadomonas sp.…”

Section: Discussionsupporting

confidence: 86%

Biosynthesis of Novel Tellurium Nanorods by Gayadomonas sp. TNPM15 Isolated from Mangrove Sediments and Assessment of Their Impact on Spore Germination and Ultrastructure of Phytopathogenic Fungi

et al. 2023

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The biosynthesis of nanoparticles using green technology is emerging as a cost-efficient, eco-friendly and risk-free strategy in nanotechnology. Recently, tellurium nanoparticles (TeNPs) have attracted growing attention due to their unique properties in biomedicine, electronics, and other industrial applications. The current investigation addresses the green synthesis of TeNPs using a newly isolated mangrove-associated bacterium, Gayadomonas sp. TNPM15, and their impact on the phytopathogenic fungi Fusarium oxysporum and Alternaria alternata. The biogenic TeNPs were characterized using transmission electron microscopy (TEM), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), Raman spectroscopy and Fourier transform infrared (FTIR). The results of TEM revealed the intracellular biosynthesis of rod-shaped nanostructures with a diameter range from 15 to 23 nm and different lengths reaching up to 243 nm. Furthermore, the successful formation of tellurium nanorods was verified by SEM-EDX, and the XRD pattern revealed their crystallinity. In addition, the FTIR spectrum provided evidence for the presence of proteinaceous capping agents. The bioinspired TeNPs exhibited obvious inhibitory effect on the spores of both investigated phytopathogens accomplished with prominent ultrastructure alternations, as evidenced by TEM observations. The biogenic TeNPs impeded spore germination of F. oxysporum and A. alternata completely at 48.1 and 27.6 µg/mL, respectively. Furthermore, an increase in DNA and protein leakage was observed upon exposure of fungal spores to the biogenic TeNPs, indicating the disruption of membrane permeability and integrity. Besides their potent influence on fungal spores, the biogenic TeNPs demonstrated remarkable inhibitory effects on the production of various plant cell wall-degrading enzymes. Moreover, the cytotoxicity investigations revealed the biocompatibility of the as-prepared biogenic TeNPs and their low toxicity against the human skin fibroblast (HSF) cell line. The biogenic TeNPs showed no significant cytotoxic effect towards HSF cells at concentrations up to 80 μg/mL, with a half-maximal inhibitory concentration (IC50) value of 125 μg/mL. The present work spotlights the antifungal potential of the biogenic TeNPs produced by marine bacterium against phytopathogenic fungi as a promising candidate to combat fungal infections.

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Section: Discussionsupporting

confidence: 86%

Biosynthesis of Novel Tellurium Nanorods by Gayadomonas sp. TNPM15 Isolated from Mangrove Sediments and Assessment of Their Impact on Spore Germination and Ultrastructure of Phytopathogenic Fungi

et al. 2023

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“…Another important factor is the nanoparticle concentration. 146,147 This property is directly correlated to microbial species. 56,79 Kim et al reported that the antibacterial activity of AgNPs was more effective against E. coli than against S. aureus.…”

Section: Factors Affecting the Antimicrobial Activity Of Silver Nanopmentioning

confidence: 99%

Green synthesis of silver nanoparticles: biomolecule-nanoparticle organizations targeting antimicrobial activity

et al. 2019

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“…Proteus spp. at 10 μg/mL and 16 μg/mL as reported by Parveen et al and Raheem et al[53,54], and at 0.015 mg/ mL as investigated by Junevičius and co-workers[55]. But the MIC values reached to 50 mg/mL when Proteus spp.…”

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confidence: 67%

Down-Regulation of fliL Gene Expression by Ag Nanoparticles and TiO2 Nanoparticles in Pragmatic Clinical Isolates of Proteus mirabilis and Proteus vulgaris from Urinary Tract Infection

Saleh¹,

Malik²,

Al-Rubaii³

2019

Nano BioMed ENG

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Ten isolates belonging to Proteus spp. were collected and obtained from Department of Biology, College of Science, the University of Baghdad. The diagnosis was done by polymerase chain reaction (PCR) technique using 16S rRNA gene and urease C gene. All isolates (100%) were sensitive to meropenem, imipenem, ciprofloxacin, gentamycin, amoxicillin, clavulanic acid and levofloxacin. These isolates also showed 60% sensitivity to cefixime and nitrofurantoin. However, both species of P. mirabilis and P. vulgaris showed the lowest sensitivity when treated with tetracycline (60%) and amikacin (20%). Cephalothin had a variable effect on the species under study as P. mirabilis isolates were 100% sensitive in comparison with the 80% sensitivity of P. vulgaris isolates. The antibacterial activities of Ag and TiO 2 nanoparticles (NPs) were investigated. The minimum inhibitory concentration (MIC) value of Ag NPs against both species isolates was 10 mg/mL, while the MIC value of TiO 2 NPs was 14 mg/mL against P. mirabilis and 15 mg/mL against P. vulgaris isolates. P. mirabilis isolates showed larger swarming diameter than P. vulgaris, but this motility phenomenon of P. vulgaris was arrested rapidly after incubation with sub-MIC of TiO 2 NPs and Ag NPs comparatively with control. All isolates showed shifting to down-regulation in the fliL gene expression under the effect of the NPs using TiO 2 NPs and Ag NPs. In conclusion, down-regulation of the fliL gene expression is directly linked to the inhibition of swarming movement of Proteus species. We encourage using these inhibitors (after tests to ensure minimal toxicity to human) in combination with antibiotics to ensure bactericidal /bacteriostatic effect to treat Proteus infections.

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Emphasized Mechanistic Antimicrobial Study of Biofunctionalized Silver Nanoparticles on Model Proteus mirabilis

Cited by 14 publications

References 45 publications

Biosynthesis of Novel Tellurium Nanorods by Gayadomonas sp. TNPM15 Isolated from Mangrove Sediments and Assessment of Their Impact on Spore Germination and Ultrastructure of Phytopathogenic Fungi

Biosynthesis of Novel Tellurium Nanorods by Gayadomonas sp. TNPM15 Isolated from Mangrove Sediments and Assessment of Their Impact on Spore Germination and Ultrastructure of Phytopathogenic Fungi

Green synthesis of silver nanoparticles: biomolecule-nanoparticle organizations targeting antimicrobial activity

Down-Regulation of fliL Gene Expression by Ag Nanoparticles and TiO2 Nanoparticles in Pragmatic Clinical Isolates of Proteus mirabilis and Proteus vulgaris from Urinary Tract Infection

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