Employing anaerobic fungi in biogas production: challenges &amp; opportunities

Vinzelj, Julia; Joshi, Akshay; Insam, Heribert; Podmirseg, Sabine Marie

doi:10.1016/j.biortech.2019.122687

Cited by 41 publications

(16 citation statements)

References 92 publications

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“…Promising microbes and/ or CAZyme targets have been discovered in crop soil [155], compost [156], wastewater sludge [157], and herbivorous animal microbiomes [158,159]. Significantly, the anaerobic environment of the ruminant digestive tract and the termite hindgut has led to the discovery of novel species and microorganisms, including the obligate anaerobic fungi phylum Neocallimastigomycota in cattle rumen [160] and lignocellulosic microorganisms found in and cultivated by termites [159,161]. Microbial analysis of anaerobic environments is of particular interest to the bioethanol and biogas industries due to the parallels that exist between these environments.…”

Section: -Omic and Bioinformatic Approaches To Elucidate Cazyme Functionmentioning

confidence: 99%

Combined whole cell wall analysis and streamlined in silico carbohydrate-active enzyme discovery to improve biocatalytic conversion of agricultural crop residues

Tingley

Low

Xing

et al. 2021

Biotechnol Biofuels

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The production of biofuels as an efficient source of renewable energy has received considerable attention due to increasing energy demands and regulatory incentives to reduce greenhouse gas emissions. Second-generation biofuel feedstocks, including agricultural crop residues generated on-farm during annual harvests, are abundant, inexpensive, and sustainable. Unlike first-generation feedstocks, which are enriched in easily fermentable carbohydrates, crop residue cell walls are highly resistant to saccharification, fermentation, and valorization. Crop residues contain recalcitrant polysaccharides, including cellulose, hemicelluloses, pectins, and lignin and lignin-carbohydrate complexes. In addition, their cell walls can vary in linkage structure and monosaccharide composition between plant sources. Characterization of total cell wall structure, including high-resolution analyses of saccharide composition, linkage, and complex structures using chromatography-based methods, nuclear magnetic resonance, -omics, and antibody glycome profiling, provides critical insight into the fine chemistry of feedstock cell walls. Furthermore, improving both the catalytic potential of microbial communities that populate biodigester reactors and the efficiency of pre-treatments used in bioethanol production may improve bioconversion rates and yields. Toward this end, knowledge and characterization of carbohydrate-active enzymes (CAZymes) involved in dynamic biomass deconstruction is pivotal. Here we overview the use of common “-omics”-based methods for the study of lignocellulose-metabolizing communities and microorganisms, as well as methods for annotation and discovery of CAZymes, and accurate prediction of CAZyme function. Emerging approaches for analysis of large datasets, including metagenome-assembled genomes, are also discussed. Using complementary glycomic and meta-omic methods to characterize agricultural residues and the microbial communities that digest them provides promising streams of research to maximize value and energy extraction from crop waste streams.

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Section: -Omic and Bioinformatic Approaches To Elucidate Cazyme Functionmentioning

confidence: 99%

Combined whole cell wall analysis and streamlined in silico carbohydrate-active enzyme discovery to improve biocatalytic conversion of agricultural crop residues

Tingley

Low

Xing

et al. 2021

Biotechnol Biofuels

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“…The fungal ribosomal RNA (rRNA) operon-based analysis has facilitated the understanding of the diversity and phylogenetic relationships of gut fungi in natural ecosystems [ 9 ]. The internal transcribed spacer (ITS) region is usually recommended as the DNA barcode marker for gut fungi [ 10 , 11 ]. In addition, the 28S ribosomal large subunit (28S rRNA gene (LSU); D1/D2 region) might render higher coverage of early diverging lineages of fungi, such as Neocallimastigomycota , Chytridiomycota , and Mucoromycota [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

The Fibrolytic Enzyme Profiles and the Composition of Fungal Communities in Donkey Cecum-Colon Ecosystem

Zhang¹,

Wang²,

Huang³

et al. 2022

Animals

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The fibrolytic enzymes and the hindgut fungi in donkey cecum-colon ecosystem play an important role in dietary fiber digestion. A better understanding of the fibrolytic enzyme profiles and the fungal community along donkey caecum and colon is key for optimizing hindgut function. In the present study, the fibrolytic enzyme activities within donkey caecum and colon were firstly measured by spectrophotometry. Activities of carboxymethyl cellulase, avicelase, xylanase, and acetyl esterase were greater in donkey dorsal colon than in caecum, indicating that the colon microorganisms may be more efficient in producing fibrolytic enzymes compared to caecum microbes. The fungal community composition along donkey hindgut was determined by sequencing ITS region using Illumina MiSeq. Three fungal phyla were identified by sequence comparison: Ascomycota (66.8%–74.4%), Basidiomycota (21.6%–30.9%), and Neocallimastigomycota (0.9%–3.3%). The Aspergillus, Wallemia, Phanerochaete, Fusarium, and Penicillium were detected as the dominant genera, but their metabolic and functional significance in donkey cecum-colon ecosystem need further investigation. In terms of the anaerobic fungi Neocallimastigomycota, its abundance was greater in donkey colon than in caecum (p < 0.05), indicating that the donkey hindgut region was associated with differences in fungal community composition. Moreover, the relative abundance of enzymes related to plant cell wall degradation were predicted by PICRUSt, and they were also lower in caecum than in colon. The present study provided new information about fibrolytic enzyme profiles and fungal composition in donkey hindgut ecosystem.

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“…Studies analyzing the metabolites produced during AF growth are scarce and are mainly focused on the genera Piromyces and Neocallimastix . With the exact nutritional needs of AF being unknown but highly relevant for the biotechnological use of these promising organisms [ 35 ], such studies are of high importance. To the best of our knowledge any study has so far compared the produced metabolites from several AF species during growth on multiple carbon sources.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Biochemical Characterization of Six Anaerobic Fungal Strains from Zoo Animal Feces

et al. 2021

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Anaerobic fungi are prime candidates for the conversion of agricultural waste products to biofuels. Despite the increasing interest in these organisms, their growth requirements and metabolism remain largely unknown. The isolation of five strains of anaerobic fungi and their identification as Neocallimastix cameroonii, Caecomyces spec., Orpinomyces joyonii, Pecoramyces ruminantium, and Khoyollomyces ramosus, is described. The phylogeny supports the reassignment of Neocallimastix californiae and Neocallimastix lanati to Neocallimastix cameroonii and points towards the redesignation of Cyllamyces as a species of Caecomyces. All isolated strains including strain A252, which was described previously as Aestipascuomyces dubliciliberans, were further grown on different carbon sources and the produced metabolites were analyzed; hydrogen, acetate, formate, lactate, and succinate were the main products. Orpinomyces joyonii was lacking succinate production and Khoyollomyces ramosus was not able to produce lactate under the studied conditions. The results further suggested a sequential production of metabolites with a preference for hydrogen, acetate, and formate. By comparing fungal growth on monosaccharides or on the straw, a higher hydrogen production was noticed on the latter. Possible reactions to elevated sugar concentrations by anaerobic fungi are discussed.

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Employing anaerobic fungi in biogas production: challenges & opportunities

Cited by 41 publications

References 92 publications

Combined whole cell wall analysis and streamlined in silico carbohydrate-active enzyme discovery to improve biocatalytic conversion of agricultural crop residues

Combined whole cell wall analysis and streamlined in silico carbohydrate-active enzyme discovery to improve biocatalytic conversion of agricultural crop residues

The Fibrolytic Enzyme Profiles and the Composition of Fungal Communities in Donkey Cecum-Colon Ecosystem

Isolation and Biochemical Characterization of Six Anaerobic Fungal Strains from Zoo Animal Feces

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