EMS mutagenesis and characterization of Brassica rapa mutants

Abstract

Brassica rapa is an important model crop in the genus Brassica, which includes various important vegetable crops such as Chinese cabbage and turnip, and is closely related to Arabidopsis thaliana (Arabidopsis). B. rapa var. trilocularis (yellow sarson), a rapid-cycling population of B. rapa, is commonly used for genetic research and is expected to bridge the gap between the model plant Arabidopsis and Brassica crops. In this study, 940 M 2 mutagenized lines of yellow sarson were produced using ethylmethanesulfonate (EMS) mutagenesis, and these M 2 plants were examined for alterations in visible phenotypes. In total, 293 independent M 2 lines (including 2188 M 2 plants) were investigated for phenotypic alterations, and 394 individual mutants were isolated. Subsequently, the observed mutant phenotypes were classified into 8 major categories and 18 subcategories. In addition, three mutants, namely, early flowering 1 (elf1), crane-like 1 (crl1), and rosette 2 (ros2), were selected for further phenotypic and genetic analysis. elf1 and ros2 showed early and late flowering phenotypes, respectively. Based on their phenotypes, crl1 and ros2 are likely mutations in genes for auxin signaling and gibberellic acid biosynthesis, respectively.

Phenotypic, biochemical and genomic variability in generations of the rapeseed (Brassica napus L.) mutant lines obtained via chemical mutagenesis

“…Currently, producing short-stem lines is a high-priority task, and due to breeding efforts for growth limitation of lateral meristems in joints of a plant stem, plant height is considered to be an important agronomic trait. The decrease in plant height was described earlier in several EMS mutagenized populations of crops including Brassica species [63]. In the studied EMS populations of different types, we observed moderate decrease in plant height and also high variability of this feature.…”

“…These mutagens was found to induce non-lethal point DNA mutations which could be retained in the genome due to its capacity for self-pollination [60]. These induced genetic variations correlate to variability in agronomic and phenotypic traits in rapeseed mutant populations [61–63]. In the present study, EMS mutagenesis induced extensive morphological diversity among mutant progeny of canola B .…”

Phytoremediation of EMS InducedBrassica junceaHeavy Metal Hyperaccumulator Genotypes

“…Improvement of plants by genetic modifications also has beneficial impacts on phytoremediation of metal-polluted soils. Researchers induced mutation by EMS in Brassica rapa (Mizuno et al, 2014) and in Brassica juncea (Ibrahim IA, 2009) to select highly tolerant plants to some heavy metals.…”

Mutagen Ems Treatment of Microspore-Derived Embryos for Rapeseed Breeding (Brassica Napus)

“…The treatment of Brassica seeds with mutagens can cause promote the manifestation of traits such as resistance to seed shedding [1], changes in the qualitative composition of oil [2,3] changes in flowering time [4], change in seed coat color from black to yellow [5]. Additionally, haploid biotechnology is widely used in plant breeding practices, primarily for the creation of homozygous lines in one generation.…”