Discussions on prevention and control of infection Related to Health Care (IRHC) should be unrestricted and expanded to other health care facilities. The literature on infection control is traditionally based on the practice of hospital context, and there is a gap when it comes to prehospital care. In addition to health care professionals, other professionals make up the team, firefighters, police officers and others who have not taken their training specific content to provide them with knowledge about prevention and control of infections in situations with potential risk of contamination by biological fluids, therefore, education is the weapon against the spread of infectious diseases. Objective: To evaluate the effectiveness of an educational intervention using concept maps and compliance indicators for cleaning of mobile cars of Prehospital care from a capital of the midwestern region of Brazil. Method: quantitative study, quasi-experimental, data collection was from July 2014 to January 2015. The study included 34 firefighters Rescue Battalion Emergency (BSE) of the Fire Brigade of the State of Goiás working in basic and advanced life support vehicles. The study was developed in four phases: Development and content validity of compliance indicator for cleaning of vehicles; Conformity assessment of the cleaning of vehicles before training; Educational Intervention and Conformity assessment of the cleaning of vehicles after intervention. A questionnaire was applied before the intervention and 30 days after. Compliance indicators were validated by an 8 experts judge with Content Validity Index (IVC≤ 70%). The evaluation of training effectiveness was taken by McNemar test, being considered p ≤ 0.05. The training of professionals was based on the theory of meaningful learning of David Ausubel and the construction of a conceptual map designed by Joseph Novak. The data show that after the course there was a slight improvement in the number of correct questions, being statistically significant (p = 0.021) and that the course brought a homogenization knowledge given to reducing the standard deviation. Results: Of the 71 indicators of compliance, 26 were excluded, totaling 45 indicators.The pre-intervention assessment, only one (2.9%) rescuer knew cleaning definition, four (11.8%) knew the concept of disinfection, 14 (41.2%) knew the importance of sanitize the vehicle and the use of PPE. After intervention a total of three (8.8%) firefighters knew conceptualize cleanliness, five (14.7%), disinfection, on the importance of cleaning and disinfection 16 (47.1%) and of the importance of the use of PPE were 15 (44.1%). The observation of the cleaning procedure improved regarding these parameters drying after washing (p=0,004) and disinfection of the vehicle (p=0,002) Conclusion: The educational intervention using an active methodology and the construction of conceptual maps was not fully effective. The observation of the vehicles cleaning procedures remained the same pattern, except for drying and disinfecting res...