EMT, MET, Plasticity, and Tumor Metastasis

Bakir, Basil; Chiarella, Anna M.; Pitarresi, Jason R.; Rustgi, Anil K.

doi:10.1016/j.tcb.2020.07.003

Cited by 671 publications

(501 citation statements)

References 79 publications

(96 reference statements)

Supporting

Mentioning

491

Contrasting

Order By: Relevance

“…Metastasis is one of the most important characteristics of malignant tumors and often results in treatment failure and poor prognosis. 34 Mounting evidence has confirmed that ezrin and MMP2 are critical molecules involved in migration and invasion of tumors including cervical cancer. 35 , 36 In our study, quercetin enhanced the inhibitory effect of cisplatin on the migration and invasion of cervical cancer cells.…”

Section: Discussionmentioning

confidence: 99%

Effects of Quercetin on the Efficacy of Various Chemotherapeutic Drugs in Cervical Cancer Cells

Xu¹,

Xie²,

Chen³

et al. 2021

DDDT

View full text Add to dashboard Cite

Purpose This study aimed to investigate the effects of quercetin on the efficacy of various chemotherapeutic drugs in cervical cancer cells. Methods All drug experiments were performed in HeLa and SiHa cells. The cell viability was detected by Cell Counting Kit-8 assay, and cell proliferation was estimated by bromodeoxyuridine assay. CompuSyn software was utilized to calculate the combination index (CI) and evaluate the synergistic or antagonistic effect of quercetin with cisplatin, paclitaxel, 5-fluorouracil and doxorubicin on cell viability. Cell migration and invasion abilities were detected by transwell assays, and cell apoptosis was measured by flow cytometry. The expression levels of matrix metallopeptidase 2 (MMP2), ezrin, P-glycoprotein (P-Gp) and methyltransferase-like 3 (METTL3) protein treated with various drugs were analyzed by Western blotting. Results Quercetin inhibited the viability of HeLa and SiHa cells in a dose- and time-dependent manner. The CI values of quercetin with cisplatin, paclitaxel, 5-fluorouracil and doxorubicin were <1, >1, >1 and >1, respectively. The effect of combination of quercetin and cisplatin on cell proliferation was stronger than their individual effects. Co-treatment group could inhibit more cell migration and invasion in contrast to single-drug group. Besides, quercetin combined with cisplatin group induced more cell apoptosis in contrast to single-drug group. The results of Western blotting showed that the expression levels of MMP2, ezrin, P-Gp and METTL3 in co-treatment group were lower than in cisplatin group, respectively. Conclusion Quercetin and cisplatin had synergistic inhibitory effect on cervical cancer cells. Quercetin might enhance the antitumor effect of cisplatin via inhibiting proliferation, migration and invasion and elevating apoptosis through weakening MMP2, ezrin, METTL3 and P-Gp expression of cancer cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Effects of Quercetin on the Efficacy of Various Chemotherapeutic Drugs in Cervical Cancer Cells

Xu¹,

Xie²,

Chen³

et al. 2021

DDDT

View full text Add to dashboard Cite

show abstract

“…Epithelial–mesenchymal transition (EMT) is a phenomenon of epithelial cells transforming into mesenchymal cells, which is a process of cell dedifferentiation or redifferentiation [ 6 ]. Through the EMT process, the migration and motility of cancer cells are enhanced, contributing to the invasion and metastasis.…”

Section: Introductionmentioning

confidence: 99%

An epithelial–mesenchymal transition-related long noncoding RNA signature correlates with the prognosis and progression in patients with bladder cancer

Tong

Gao

et al. 2021

Bioscience Reports

View full text Add to dashboard Cite

Bladder cancer is a common malignant tumour worldwide. Epithelial–mesenchymal transition (EMT)-related biomarkers can be used for early diagnosis and prognosis of cancer patients. To explore, accurate prediction models are essential to the diagnosis and treatment for bladder cancer. In the present study, an EMT-related long noncoding RNA (lncRNA) model was developed to predict the prognosis of patients with bladder cancer. Firstly, the EMT-related lncRNAs were identified by Pearson correlation analysis, and a prognostic EMT-related lncRNA signature was constructed through univariate and multivariate Cox regression analyses. Then, the diagnostic efficacy and the clinically predictive capacity of the signature were assessed. Finally, Gene set enrichment analysis (GSEA) and functional enrichment analysis were carried out with bioinformatics. An EMT-related lncRNA signature consisting of TTC28-AS1, LINC02446, AL662844.4, AC105942.1, AL049840.3, SNHG26, USP30-AS1, PSMB8-AS1, AL031775.1, AC073534.1, U62317.2, C5orf56, AJ271736.1, and AL139385.1 was constructed. The diagnostic efficacy of the signature was evaluated by the time-dependent receiver-operating characteristic (ROC) curves, in which all the values of the area under the ROC (AUC) were more than 0.73. A nomogram established by integrating clinical variables and the risk score confirmed that the signature had a good clinically predict capacity. GSEA analysis revealed that some cancer-related and EMT-related pathways were enriched in high-risk groups, while immune-related pathways were enriched in low-risk groups. Functional enrichment analysis showed that EMT was associated with abundant GO terms or signaling pathways. In short, our research showed that the 14 EMT-related lncRNA signature may predict the prognosis and progression of patients with bladder cancer.

show abstract

“…However, SRGAP2 knockdown could not alter the expression of epithelial marker (E-cadherin) and mesenchymal markers (Ncadherin and vimentin). Although it is reported that EMT plays a critical role in rst step of the metastatic process [30], tumor metastasis also often occurs without stimulating EMT program [31]. A recent study shows that AKT-induced lncRNA VAL could promote metastasis of lung adenocarcinoma in the absence of activating EMT [32].…”

Section: Discussionmentioning

confidence: 99%

Identification of SRGAP2 as a Potential Oncogene and a Prognostic Biomarker in Hepatocellular Carcinoma Using Bioinformatics Analyses and Biological Experiments

Y¹,

Qiao²,

Bai³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Hepatocellular carcinoma (HCC) is one of the common malignancies worldwide. Slit-Robo GTPase-activating proteins (SRGAPs) have been shown to regulate the occurrence and development of various tumors. However, their specific role in HCC remains elusive.Methods: The expression pattern, genetic alteration and prognostic value of SRGAPs in HCC are analyzed by bioinformatics tools. The biological functions of SRGAP2 in HCC cells are demonstrated by in vitro experiments. The high-throughput RNA sequencing is conducted to explore the underlying molecular mechanisms of SRGAP2 in HCC cells.Results: The expression levels of SRGAP1 and SRGAP2 are significantly elevated in HCC tissues compared to the normal both in Oncomine and TCGA datasets, and SRGAP2 are dramatically upregulated both in mRNA and protein levels. Moreover, higher SRGAP2 is significantly related to the clinical stages of HCC. Meanwhile, SRGAP2 might be an independent prognostic indicator, as it correlates negatively with the clinical outcomes of HCC patients. Further SRGAP2-silencing experiments imply that SRGAP2 might remarkably promote the migration and invasion of HCC cells in EMT-independent manners. Based on the high-throughput RNA sequencing of SRGAP2-knockdown HCC cells, enrichment and network analyses demonstrate that SRGAP2 is closely associated with cellular metabolic signaling.Conclusions: Our study firstly illustrates the crucial role of SRGAP2 in the metastasis of HCC and explores its underlying molecular mechanisms. We identify SRGAP2 as a promising prognostic biomarker and a novel therapeutic target for HCC patients.

show abstract

EMT, MET, Plasticity, and Tumor Metastasis

Cited by 671 publications

References 79 publications

Effects of Quercetin on the Efficacy of Various Chemotherapeutic Drugs in Cervical Cancer Cells

Effects of Quercetin on the Efficacy of Various Chemotherapeutic Drugs in Cervical Cancer Cells

An epithelial–mesenchymal transition-related long noncoding RNA signature correlates with the prognosis and progression in patients with bladder cancer

Identification of SRGAP2 as a Potential Oncogene and a Prognostic Biomarker in Hepatocellular Carcinoma Using Bioinformatics Analyses and Biological Experiments

Contact Info

Product

Resources

About