Emulsification of Hydrocarbons by Biosurfactant: Exclusive Use of Agrowaste

Kinetic modelling of cell growth, substrate utilization, and biosurfactant production from solid agrowaste (Beta vulgaris) by Bacillus licheniformis STK 01

“…Bacillus licheniformis STK 01 [Accession Number KR011152] was isolated in our research laboratory from decaying wood and identified by morphological as well as 16S ribosomal deoxyribonucleic acid (rDNA) sequencing analysis. The bacteria strain was capable of producing biosurfactant under mesophilic growth conditions . The isolate was maintained on nutrient agar slants at 4 °C and subcultured every three weeks.…”

“…In most cases, these wastes are supplemented with refined carbon sources and trace elements to make up the proportion required for microbial growth and synthesis of requisite biological products. However, recent studies have shown that these agrowastes can be used exclusively by microorganisms as growth substrates for biosurfactant synthesis, without supplementation with refined carbon sources or nutrient elements . This is particularly suitable when the biological systems are designed for contaminant bioremediation, in either in‐situ or ex‐situ bioremediation settings.…”

“…However, a recent report has shown that these solid residues could sustain microbial metabolic functions during biosurfactant production . An example of such an application is in composting of organic waste for enhanced soil remediation .…”

“…In our previous studies a novel Bacillus licheniformis strain STK 01 [GenBank Accession Number KR011152] was identified that was able to utilize Beta vulgaris waste exclusively for biosurfactant production and subsequently the culture parameters were optimized . The objective of this work was therefore to investigate the kinetics of B. vulgaris waste sugar utilization by B. licheniformis STK 01, and the subsequent biosurfactant production under optimized conditions.…”

Biodegradation Kinetics of Free Cyanide in Fusarium oxysporum-Beta vulgaris Waste-metal (As, Cu, Fe, Pb, Zn) Cultures under Alkaline Conditions

“…The high degradation rate observed may be attributed to the sufficient enzyme activity aided by suitable bioreactor conditions and the utilisation of Beta vulgaris waste extract which contains soluble sugars, minerals and proteins to support enzyme production. Earlier reports have shown that substrates such as Beta vulgaris have a large content in pectin, reducing sugars, cellulose, and protein (Mulligan 2005;Anuradha et al 2010;Amodu et al 2014). Anuradha et al (2010) showed that Fusarium oxysporum is capable of producing numerous enzymes when grown on Beta vulgaris, orange peel, carrot peel, and pineapple peel.…”