Emulsion Electrospun Fiber Mats of PCL/PVA/Chitosan and Eugenol for Wound Dressing Applications

Abstract

In recent years, the damaging effects of antimicrobial resistance relating to wound management and infections have driven the ongoing development of composite wound dressing mats containing natural compounds, such as plant extracts and their derivatives. The present research reports the fabrication of novel electrospun Polycaprolactone (PCL)/Polyvinyl Alcohol (PVA)/Chitosan (CS) fiber mats loaded with Eugenol (EUG), an essential oil, known for its therapeutic properties. The electrospun fiber mats were prepared via electrospinning from either water-in-oil (W/O) or oil-in-water (O/W) emulsions and characterized using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), total porosity measurements, and water contact angle. The in vitro EUG release profile and antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa were also evaluated. The obtained results proved that the EUG was loaded efficiently into electrospun PCL/PVA/CS fiber mats and the two W/O and O/W emulsions prepared from the PCL/PVA/CS (7 : 3 : 1) and PCL/PVA/CS (3 : 7 : 1) revealed porosity within the ideal range of 60–90%, even when EUG was loaded. The measured contact angle values showed that the O/W emulsion exhibited a more hydrophilic character and the wettability noticeably decreased after adding EUG in both emulsion blends. Furthermore, the electrospun PCL/PVA/CS fiber mats demonstrated a rapid release of EUG during the first 8 hours, which enhanced gradually afterward (up to 120 hours). Moreover, an efficient antibacterial activity against S. aureus (inhibition ratios of 92.43% and 83.08%) and P. aeruginosa (inhibition ratios of 94.68% and 87.85%) was displayed and the in vitro cytotoxic assay demonstrated that the normal human dermal fibroblasts (NHDF) remained viable for at least 7 days, after direct contact with the produced electrospun fiber mats. Therefore, such findings support the biocompatibility and suitability of using these EUG-loaded electrospun PCL/PVA/CS fiber mats as a new innovative wound dressing material with potential for preventing and treating microbial wound infections.

Tensile Properties of Composite Reinforced with Three-Dimensional Printed Fibers

“…Similarly, the intensity of the peak at 2940 cm −1 in the composite corresponding to the -CH-group increased in comparison to the peak recorded in the spectra of PCL. These results indicate that there is some degree of interaction between carboxyl and hydroxyl groups of the PCL fibers and the PVA matrix [36].…”

Co‐Axial Gyro‐Spinning of PCL/PVA/HA Core‐Sheath Fibrous Scaffolds for Bone Tissue Engineering

“…Surface wettability is regarded as one of the desired material properties in tissue engineering and wound dressings, which may influence cell adhesion and migration in the spun fibers. [ 56 ] In addition, cellular proliferation is related to the hydrophilicity of the materials hence it may promote the rate of cellular proliferation. [ 57 ] Therefore, to assess the surface wettability and hydrophilicity of the spun fibers and the effect of HA on those properties, the water contact angle was measured and compared with pure PCL–PVA core‐sheath fibers.…”

Nanostructured Fibers Containing Natural or Synthetic Bioactive Compounds in Wound Dressing Applications

“…Mouro [98] fabricated novel electrospun PCL/PVA/CS mats loaded with 5% w/w Eugenol (EUG), using W/O and O/W emulsions, for wound dressing applications. The release profile of EUG from electrospun fiber mats was investigated and the results revealed a rapid release of EUG during the first 8 h and afterwards the release enhanced gradually over the next days (up to 120 h).…”