Emulsion encapsulation of Bifidobacterium animalis subsp. lactis Bb12 with the addition of lecithin

Abstract

Lisová I., Horáčková Š., Kováčová R., Rada V., Plocková M. (2013): Emulsion encapsulation of Bifidobacterium animalis subsp. lactis Bb12 with the addition of lecithin. Czech J. Food Sci., 31: 270-274.The commercial probiotic strain Bifidobacterium animalis subsp. lactis Bb12 was encapsulated using emulsion encapsulation into milk protein matrix without and with the addition of 0.5% w/w lecithin into the oil. Different agitation speeds were used during the encapsulation process. The examination of microcapsules was carried out by optical microscope and fluorescence in situ hybridisation. The particle size distribution as volume based median d 0.5 was evaluated by the laser diffraction method. In the case of no lecithin addition, the agitation speed did not influence significantly the size of the microcapsules. The addition of 0.5% (w/w) of lecithin into the oil caused a decrease of d 0.5 value from 196 ± 37 µm to 79 ± 3 µm at an agitation speed of 500 rpm, and from 193 ± 24 µm to 39 ± 3 µm at 1200 rpm. It can improve the sensory properties of the products with the added microcapsules.

Preparation and properties of milk proteins-based encapsulated probiotics: a review

“…Addition of lecithin and changing agitation speed during encapsulation of B. animalis subsp. lactis Bb12 using the method of Heidebach et al (2009b) resulted in microcapsules of smaller diameter (Lisová et al 2013).…”

Lecithin - Bifidobacterium probiotics interactions: a case study

“…Furthermore, lecithin has also been used as an excipient in probiotics formulations. When lecithin and other coating materials, such as alginate or milk proteins were used, the probiotics showed higher cell viability after storage, and higher resistance to gastric conditions (Albadran et al, 2018;Bollom et al, 2020;Donthidi et al, 2010a;Huq et al, 2017;Lisová et al, 2013). Lecithin has also been proven to preserve the viability of probiotic cells, such as Lactobacillus plantarum strains and Bifidobacterium lactis, against the action of bile salts, due to the protective effect of the self-assembled lecithin vesicles (Chen et al, 2012;Hu et al, 2015).…”

Influence of co-encapsulation of Bifidobacterium animalis subsp. lactis Bb12 with inulin and ascorbic acid on its viability

“…encapsulation process. Emulsion encapsulation was done according to Heidebach et al (2009) and Lisová et al (2013) with some modifications. In brief, 120 g of 35% (w/w) reconstituted skimmed milk (Laktino skimmed milk powder, Promil, Nový Bydžov, Czech Republic) was mixed with fresh culture from 200 mL cultivation in MRS (de Man, Rogosa and Sharpe medium).…”