Emycin-E purified from Streptomyces sp. RG1011 from Himalayan soil has antibiofilm activity against Staphylococcus aureus

Sankhwar, Ruchi; Kumar, Abhishek; Yadav, Shilpi; Singh, Vineeta; Gupta, Ravi Kr.

doi:10.1016/j.micpath.2023.106256

Cited by 3 publications

(3 citation statements)

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“…We have explored the growth pattern, biomass production and antimicrobial properties of the potent isolate Bacillus licheniformis RG1002 for 10 days as described previously. 25,26 The seed culture of the strain RG1002 was grown in 5 ml nutrient broth (NB) medium for overnight and diluted 100x in fresh 100 ml NB medium next day in a 500 ml flask. The flask was kept in shaker incubator at 150 rpm for 10 days at 37°C.…”

Section: Growth Kinetics and Time Dependent Antimicrobial Activitymentioning

confidence: 99%

“…The minimum inhibitory concentration (MIC) was calculated against the various pathogens via 96-well microtiter plate technique described earlier with some minor modifications. 26,27 S. flexneri, S. typhi, E. coli and P. aeruginosa were grown for in LB (Luria Bertani broth) and S. aureus and B. subtilis were grown in TSB (tryptic soya broth) for 24 hr. Next day, each bacterial culture was diluted 1000 times in a fresh culture medium and 150 µl broth culture was inoculated in every well of a 96-well plate in triplicate.…”

Section: Mic and Oil Displacement Assay Of Purified Lichenysinmentioning

confidence: 99%

“…Biofilm inhibition formed by P. aeruginosa with or without purified lichenysin was quantitatively measured by crystal violet dye as described earlier with some minor modifications. 26 Briefly, P. aeruginosa was grown in LB at 37°C and 150 rpm for 24 hr. Next day, culture was diluted One hundred times in fresh LB with 0.25% glucose (Biofilm medium) and 150 µl was aliquoted in 96 well plate in triplicate with 25 µg/ml (MIC) and 12.5 µg/ml (1/2 of the MIC) lichenysin except negative controls which have only biofilm medium and positive controls have P. aeruginosa in biofilm medium.…”

Section: Quantitative Biofilm Assaymentioning

confidence: 99%

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Lichenysin Produced by Bacillus licheniformis RG1002 Inhibit the Biofilm Formation of Pseudomonas aeruginosa on Various Surfaces

Kumar,

Sankhwar,

Yadav

et al. 2024

J. Pure Appl. Microbiol.

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Pseudomonas aeruginosa is capable of producing biofilms on various surfaces, medical implants and burn wounds which inhibit the action of antimicrobial agents. This study was undertaken for the screening, identification and purification of a natural antimicrobial compound from the hospital waste sites against P. aeruginosa. The partial purification of the antimicrobial agent was performed with ethyl acetate from the culture supernatant of the isolate and further purified by HPLC. For the characterization of the active compound, we have used Fourier Transform Infrared Spectroscopy (FT-IR) and Electrospray Ionization mass spectrometry (ESI-MS) analysis. The purified compound was tested for anti-biofilm activity in vitro against P. aeruginosa and on various surfaces such as plastic, glass, and steel. The potent isolate was confirmed as Bacillus licheniformis strain RG1002. The HPLC purified compound was characterized as lichenysin using FTIR and ESI-MS analysis. This study identifies and characterize the potent antimicrobial agent against other important human pathogens such as Staphylococcus aureus, Salmonella typhi, Shigella flexneri and Escherichia coli. Purified lichenysin showed strong inhibitory effect against P. aeruginosa and other important pathogens and inhibits the biofilm formation of P. aeruginosa in vitro on various types of surfaces. The current study suggests the application of lichenysin as a protective coating on various indwelling medical devices and catheters to prevent the biofilm formation by P. aeruginosa.

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Section: Growth Kinetics and Time Dependent Antimicrobial Activitymentioning

confidence: 99%

Section: Mic and Oil Displacement Assay Of Purified Lichenysinmentioning

confidence: 99%

Section: Quantitative Biofilm Assaymentioning

confidence: 99%

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