En bloc transfer of extracellular matrix<i>in vitro</i>

Tc, Ho; Lv, Del Priore; Hj, Kaplan

doi:10.3109/02713689609017645

Cited by 18 publications

(12 citation statements)

References 22 publications

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“…Culture of bovine retinal epithelium (RPE) cells was obtained, basically, as described previously [Ho et al, 1996]. Freshly enucleated eyes were obtained from a local slaughterhouse within 2 h of death.…”

Section: Cell Culturesmentioning

confidence: 99%

Gamma synuclein: Subcellular localization in neuronal and non‐neuronal cells and effect on signal transduction

Surguchov

Palazzo

Surgucheva

2001

Cell Motil. Cytoskeleton

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Synucleins are small, highly conserved proteins in vertebrates, especially abundant in neurons and typically enriched in presynaptic terminals. alpha-Synuclein protein and a fragment of it, called NAC, have been found in association with pathological lesions of neurodegenerative diseases. Recently, mutations in a alpha-synuclein gene have been reported in families susceptible to an inherited form of Parkinson's diseases. In addition, alpha-synuclein has been implicated in the pathophysiology of other neurodegenerative diseases, including Alzheimer's disease and multiple system atrophy. Far less is known about other members of the synuclein family, beta- and gamma-synucleins. gamma-synuclein is up-regulated in several types of cancer and may affect the integrity of the neurofilament network, while its bovine ortholog, synoretin, activates the Elk-1 signal transduction pathway. In this paper, we present data about the localization and properties of human and bovine gamma-synuclein in several neuronal and non-neuronal cell cultures derived from ocular tissues. We show that gamma-synuclein is present in the perinuclear area and is localized to centrosomes in several types of human interphase cells and in bovine retinal pigment epithelium. In mitotic cells, gamma-synuclein staining is localized to the poles of the spindle. Further, overexpression of synoretin in retinoblastoma cells up-regulates MAPK and Elk-1. These results support the view that gamma-synuclein is a centrosome protein that may be involved in signal transduction pathways.

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Section: Cell Culturesmentioning

confidence: 99%

Gamma synuclein: Subcellular localization in neuronal and non‐neuronal cells and effect on signal transduction

Surguchov

Palazzo

Surgucheva

2001

Cell Motil. Cytoskeleton

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“…In addition, gelatin, which is collagen that has been processed to remove its higher organized structure, has been widely studied with RPE [24][25][26]. To the best of our knowledge, collagen type IV has been assessed with RPE cells and hESC-RPE cells merely in the form of protein coating [27][28][29].…”

Section: Introductionmentioning

confidence: 99%

Biomimetic collagen I and IV double layer Langmuir–Schaefer films as microenvironment for human pluripotent stem cell derived retinal pigment epithelial cells

et al. 2015

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Skottman, H. (2015). Biomimetic collagen I and IV double layer Langmuir-Schaefer films as microenvironment for human pluripotent stem cell derived retinal pigment epithelial cells. BIOMATERIALS, 51, 257-269. DOI: 10.1016/j.biomaterials.2015 AbstractThe environmental cues received by the cells from synthetic substrates in vitro are very different from those they receive in vivo. In this study, we applied the Langmuir-Schaeffer (LS) deposition, a variant of Langmuir-Blodgett technique, to fabricate a biomimetic microenvironment mimicking the structure and organization of native Bruch's membrane for the production of the functional human embryonic stem cell derived retinal pigment epithelial (hESC-RPE) cells. Surface pressure-area isotherms were measured simultaneously with Brewster angle microscopy to investigate the self-assembly of human collagens type I and IV on airsubphase interface. Furthermore, the structure of the prepared collagen LS films was characterized with scanning electron microscopy, atomic force microscopy, surface plasmon resonance measurements and immunofluorescent staining. The integrity of hESC-RPE on double layer LS films was investigated by measuring transepithelial resistance and permeability of small molecular weight substance. Maturation and functionality of hESC-RPE cells on double layer collagen LS films was further assessed by RPE-specific gene and protein expression, growth factor secretion, and phagocytic activity. Here, we demonstrated that the prepared collagen LS films have layered structure with oriented fibers corresponding to architecture of the uppermost layers of Bruch's membrane and result in increased barrier properties and functionality of hESC-RPE cells as compared to the commonly used dip-coated controls.

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“…Collagen has been widely investigated as a scaffold for a variety of tissue types and can be crosslinked into gels of various mechanical properties and degradation rates [50,53]. Gelatin, collagen that has been processed to remove its higher order structure [54], has been examined for both RPE and photoreceptor transplants [55,56]. Fibrin is a primary component of blood clots [57] and consists of fibrinogen that has been crosslinked in the presence of thrombin to form a dense mesh network [58].…”

Section: Materials For Scaffoldsmentioning

confidence: 99%

A tissue-engineered approach towards retinal repair: Scaffolds for cell transplantation to the subretinal space

Hynes

Lavik

2010

Graefes Arch Clin Exp Ophthalmol

160

135

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En bloc transfer of extracellular matrixin vitro

Cited by 18 publications

References 22 publications

Gamma synuclein: Subcellular localization in neuronal and non‐neuronal cells and effect on signal transduction

Gamma synuclein: Subcellular localization in neuronal and non‐neuronal cells and effect on signal transduction

Biomimetic collagen I and IV double layer Langmuir–Schaefer films as microenvironment for human pluripotent stem cell derived retinal pigment epithelial cells

A tissue-engineered approach towards retinal repair: Scaffolds for cell transplantation to the subretinal space

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