Enabling comprehensive optogenetic studies of mouse hearts by simultaneous opto-electrical panoramic mapping and stimulation

Rieger, Michael; Dellenbach, Christian; Berg, Johannes vom; Beil-Wagner, Jane; Maguy, Ange; Rohr, Stephan

doi:10.1038/s41467-021-26039-8

Cited by 9 publications

(7 citation statements)

References 45 publications

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“…In the realm of basic scientific researches, scholars have advanced the field by developing a comprehensive view of photoelectric measurement for the hearts of rats and a corresponding stimulation system. 122 At the heart of this system lies a configuration of 294 optical fibers and 64 electrodes that span the entirety of the heart's ventricular surface and vessels in mice. This system allows for the precise customization of experiments to meet specific requirements.…”

Section: Genetic and Heart Light Systemmentioning

confidence: 99%

“…The main research direction includes basic science research and treatment of heart diseases. In the realm of basic scientific researches, scholars have advanced the field by developing a comprehensive view of photoelectric measurement for the hearts of rats and a corresponding stimulation system 122 . At the heart of this system lies a configuration of 294 optical fibers and 64 electrodes that span the entirety of the heart's ventricular surface and vessels in mice.…”

Section: Applications Of Optogenetics In Various Fieldsmentioning

confidence: 99%

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Emerging optogenetics technologies in biomedical applications

Ren,

Cheng,

Wen

et al. 2023

Smart Medicine

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Optogenetics is a cutting‐edge technology that merges light control and genetics to achieve targeted control of tissue cells. Compared to traditional methods, optogenetics offers several advantages in terms of time and space precision, accuracy, and reduced damage to the research object. Currently, optogenetics is primarily used in pathway research, drug screening, gene expression regulation, and the stimulation of molecule release to treat various diseases. The selection of light‐sensitive proteins is the most crucial aspect of optogenetic technology; structural changes occur or downstream channels are activated to achieve signal transmission or factor release, allowing efficient and controllable disease treatment. In this review, we examine the extensive research conducted in the field of biomedicine concerning optogenetics, including the selection of light‐sensitive proteins, the study of carriers and delivery devices, and the application of disease treatment. Additionally, we offer critical insights and future implications of optogenetics in the realm of clinical medicine.

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Section: Genetic and Heart Light Systemmentioning

confidence: 99%

Section: Applications Of Optogenetics In Various Fieldsmentioning

confidence: 99%

Emerging optogenetics technologies in biomedical applications

Ren,

Cheng,

Wen

et al. 2023

Smart Medicine

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“…If significant curvature of the tissue persists within the mapped region then wavefronts moving in three dimensions can impact measurements of conduction velocity ( 169 ). Technical innovations have enabled multidimensional optical mapping, including panoramic mapping of the entire epicardium ( 169 , 322 , 323 ), simultaneous optical and electrical panoramic mapping of mouse hearts ( 324 ), and transmural mapping of thick tissue using transillumination ( 325 ) and near-infrared indicators ( 326 , 327 ).…”

Section: Experimental Approachesmentioning

confidence: 99%

Guidelines for assessment of cardiac electrophysiology and arrhythmias in small animals

Ripplinger

Glukhov

Kay

et al. 2022

American Journal of Physiology-Heart and Circulatory Physiology

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Cardiac arrhythmias are a major cause of morbidity and mortality worldwide. Although recent advances in cell-based models, including human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CM), are contributing to our understanding of electrophysiology and arrhythmia mechanisms, pre-clinical animal studies of cardiovascular disease remain a mainstay. Over the past several decades, animal models of cardiovascular disease have advanced our understanding of pathological remodeling, arrhythmia mechanisms, drug effects, and have led to major improvements in pacing and defibrillation therapies. There exist a variety of methodological approaches for assessment of cardiac electrophysiology, and a plethora of parameters may be assessed with each approach. This Guidelines article will provide an overview of the strengths and limitations of several common techniques used to assess electrophysiology and arrhythmia mechanisms at the whole animal, whole heart, and tissue level, with a focus on small animal models. We also define key electrophysiological parameters that should be assessed, along with their physiological underpinnings, and the best methods with which to assess these parameters.

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“…Currently, cardiac optogenetics is primarily used in in vitro and ex vivo cardiac studies with cell cultures or explanted perfused hearts. [13][14][15][16][17] More in vivo research is crucially needed to fully exploit the unique opportunities cardiac optogenetics offers for mechanistic investigations of heart function in health and disease.Small animal models such as mice and rats are the main rodent species that could be genetically engineered for in vivo cardiac optogenetics research and their heart electrophysiology is a good approximation of the human electrophysiology. [18] Implantable cardiac devices that combine precise light delivery to targeted heart regions of small animals with electrophysiological readout capabilities remain a major technological Bioelectronic devices that allow simultaneous accurate monitoring and control of the spatiotemporal patterns of cardiac activity provide an effective means to understand the mechanisms and optimize therapeutic strategies for heart disease.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

Flexible Electro‐Optical Arrays for Simultaneous Multi‐Site Colocalized Spatiotemporal Cardiac Mapping and Modulation

Obaid

Chen

Madrid

et al. 2022

Advanced Optical Materials

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disease arises from asynchrony and abnormalities in the complex and coordinated electro-mechanical properties over time and space. Therefore, devices that allow monitoring and controlling of the spatiotemporal dynamics of cardiac activity are crucial for unraveling the pathophysiology of heart disease and developing effective treatment therapies in clinical cardiology practice. Implantable electronic pacemakers play an essential role in treating various types of arrhythmias and heart failure and studying cardiac physiology by changing the membrane potential and triggering an action potential with an electric current. [3,4] However, electrical stimulation can lead to adverse effects on cell health and integrity due to the cell membrane electroporation and redox processes. [5,6] The electrical fields created by the stimulation electrodes will generate electrical crosstalk between stimulation and recording electrodes and result in recording artifacts. [7] Furthermore, electrical stimulation is unable to target specific subtypes of cardiac cells. Optogenetics uses light to modulate the activity of genetically targeted cell types through photosensitive ion channels and pumps. [8] Despite its initial use in neuroscience research to control neural circuits, [8,9] optogenetics has now been applied as a promising tool in cardiology for pain-free, low-energy optical pacing, and defibrillation with cell-type specificity. [10][11][12] In addition, cardiac optogenetics generally interferes less with simultaneous electrical readout of cardiac activity compared to electrical stimulation. Currently, cardiac optogenetics is primarily used in in vitro and ex vivo cardiac studies with cell cultures or explanted perfused hearts. [13][14][15][16][17] More in vivo research is crucially needed to fully exploit the unique opportunities cardiac optogenetics offers for mechanistic investigations of heart function in health and disease.Small animal models such as mice and rats are the main rodent species that could be genetically engineered for in vivo cardiac optogenetics research and their heart electrophysiology is a good approximation of the human electrophysiology. [18] Implantable cardiac devices that combine precise light delivery to targeted heart regions of small animals with electrophysiological readout capabilities remain a major technological Bioelectronic devices that allow simultaneous accurate monitoring and control of the spatiotemporal patterns of cardiac activity provide an effective means to understand the mechanisms and optimize therapeutic strategies for heart disease. Optogenetics is a promising technology for cardiac research due to its advantages such as cell-type selectivity and high space-time resolution, but its efficacy is limited by the insufficient number of modulation channels and lack of simultaneous spatiotemporal mapping capabilities in current implantable cardiac optogenetics tools available for in vivo investigations. Here, soft implantable electro-optical cardiac devices integrating multilayered highly ...

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Enabling comprehensive optogenetic studies of mouse hearts by simultaneous opto-electrical panoramic mapping and stimulation

Cited by 9 publications

References 45 publications

Emerging optogenetics technologies in biomedical applications

Emerging optogenetics technologies in biomedical applications

Guidelines for assessment of cardiac electrophysiology and arrhythmias in small animals

Flexible Electro‐Optical Arrays for Simultaneous Multi‐Site Colocalized Spatiotemporal Cardiac Mapping and Modulation

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