Enantiodetermination of R,S-3,4-methylenedioxypyrovalerone in urine samples by high pressure in-line solid-phase extraction capillary electrophoresis-mass spectrometry

Pérez‐Alcaraz, Albert; Borrull, Francesc; Aguilar, Carme; Calull, Marta; Benavente, Fernando

doi:10.1016/j.talanta.2020.121994

Cited by 24 publications

(9 citation statements)

References 31 publications

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“…Two different BGEs-10 mM ammonium acetate and 1 M HCOOH-were evaluated in terms of resolution. This was performed considering their compatibility with the MS system and taking into account that they are both widely employed BGEs in the determination of ATS by CE-MS [21,35,40,41]. Table 2 shows the resolution between the different ATS included in this study using the two aforementioned BGEs.…”

Section: Separation and Detection Optimizationmentioning

confidence: 99%

“…The advantages of CE in chiral analyses are related to the rapid method development and its flexibility when it comes to choosing or changing the chiral selector and its concentration, since the enantioseparation is performed simply by adding this substance to the BGE [11,30,[32][33][34]. However, when a chiral CE method is combined with MS it is important to take into account that, apart from volatile and low conductivity BGEs, compatible chiral selectors to prevent source dirtying should be used since this can cause problems when low sensitivity is required [29,30,34,35]. Although CDs are the most common chiral selectors used in CE, they are nonvolatile compounds and may bring about source contamination of the MS system.…”

Section: Introductionmentioning

confidence: 99%

“…Although lowering the concentration of the chiral selector can minimize these drawbacks, different strategies have been reported in the literature as regards minimizing the contamination of the ionization source [34]. One of these is the countercurrent migration technique (CMT), in which the selected chiral selector migrates in the opposite direction to the EOF [35,36]. To achieve this, charged chiral selectors are required.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of different chiral selectors for the enantiomeric determination of amphetamine‐type substances in human urine by solid‐phase extraction followed by capillary electrophoresis‐tandem mass spectrometry

et al. 2021

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The present study develops a method for the enantioseparation of a group of amphetamines and their metabolites in urine by CE coupled to MS/MS (CE-MS/MS). Amphetamines present a chiral center and thus two enantiomers, which is important from a toxicological point of view because they may have different pharmacokinetic and pharmacological properties. It is therefore essential to find suitable methods to distinguish both enantiomers. Today the use of CE is becoming more important in this field since, with the simple addition of a chiral selector to the background electrolyte, the enantioseparation can easily be achieved. However, when CE is coupled to MS, the use of volatile chiral selectors and compatible background electrolytes or other strategies such as the countercurrent migration approach are required to avoid contamination of the ion source from nonvolatile species. In the present study, we use the latter strategy to evaluate six different chiral selectors using CE-MS/MS. As a sample pre-treatment, two cationic-exchange sorbents-Oasis WCX and Oasis MCX-are compared for the urine pre-treatment. Using this method, it was possible to achieve the complete chiral separation of the amphetamines under study with detection limits ranging between 0.8 and 1.5 ng/mL and method quantification limits between 2.0 and 8.0 ng/mL. Matrix-matched calibration curves up to 150 ng/mL were used to cover the usual concentration ranges at which amphetamines have generally been found in toxicological and forensic analyses.

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Section: Separation and Detection Optimizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparison of different chiral selectors for the enantiomeric determination of amphetamine‐type substances in human urine by solid‐phase extraction followed by capillary electrophoresis‐tandem mass spectrometry

et al. 2021

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“…In this way, no suppression of the signal was observed. Figure 1 shows the enantioseparation of racemic R,S ‐MDPV spiked to a urine sample and using an in‐line SPE‐CE‐MS [26].…”

Section: Analysis Of Drugs Of Abuse By Using Miniaturized Techniquesmentioning

confidence: 99%

“…Table 1 reports selected applications by using miniaturized techniques [26][27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44].…”

Section: Selected Applications Of Miniaturized Techniques Used For Th...mentioning

confidence: 99%

Potentiality of miniaturized techniques for the analysis of drugs of abuse

et al. 2021

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Capillary electromigration (CE) and liquid chromatographic techniques (CLC/nano‐LC) are miniaturized techniques offering distinct advantages over conventional ones in the field of separation science. Among these, high efficiency, high chromatographic resolution, and use of minute volumes of both mobile phase and sample volumes are the most important. CE and CLC/nano‐LC have been applied to the analysis of many compounds including peptides, proteins, drugs, enantiomers, ions, etc. Over the years, the methods described here have also been used for the analysis of compounds of clinical, forensic, and toxicological interest. In this review article, the main features of the mentioned techniques are summarized. Their potentiality for the analysis of drugs of abuse are discussed. Some selected applications in this field in the period of 2015–present are also reported.

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Development of a sensitive MEKC‐LIF method for synthetic cathinones analysis

et al. 2021

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Synthetic cathinones are phenylalkylamine compounds related to natural cathinone from Catha edulis leaves. Due to their sympathomimetic effects comparable to common illicit drugs, these substances are mainly drugs of abuse and constitute the second most frequently seized group of new psychoactive substances. In order to ensure their regulation and to promote public health, reliable analytical tools are required to track these substances. In the present study, we developed a CE hyphenated to laser-induced fluorescence detection method to demonstrate its suitability to perform fast and cost-effective synthetic cathinones analysis. Fourteen compounds including isobaric compounds and position isomers were selected to encompass the large panel of chemical structures. To separate the FITC-labeled analytes (presenting the same negative charge and close mass to charge ratios), MEKC separation mode was selected. Method selectivity was not suitable using common surfactants. In this context, alkyl polyethylene glycol ether surfactants were successfully used as neutral surfactant to overcome this analytical challenge. The effect of surfactant nature on separation performances and migration behaviors of the analytes was also studied. Optimal BGE composition included 75 mM borate buffer at pH 9.3 and 0.4 mM of C12E10 surfactant. Final MEKC separation conditions were proposed to analyze a large panel of synthetic cathinones. This method helped to reach a sensitivity with LOD from 0.1 to 0.4 nM (pg/mL order).

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Enantiodetermination of R,S-3,4-methylenedioxypyrovalerone in urine samples by high pressure in-line solid-phase extraction capillary electrophoresis-mass spectrometry

Cited by 24 publications

References 31 publications

Comparison of different chiral selectors for the enantiomeric determination of amphetamine‐type substances in human urine by solid‐phase extraction followed by capillary electrophoresis‐tandem mass spectrometry

Comparison of different chiral selectors for the enantiomeric determination of amphetamine‐type substances in human urine by solid‐phase extraction followed by capillary electrophoresis‐tandem mass spectrometry

Potentiality of miniaturized techniques for the analysis of drugs of abuse

Development of a sensitive MEKC‐LIF method for synthetic cathinones analysis

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