Enantiomeric Determination of DOPA in Dietary Supplements Containing &lt;i&gt;Mucuna pruriens&lt;/i&gt; by Liquid Chromatography/Mass Spectrometry

Hasegawa, Takashi; Takahashi, Kazunaga; Fukiwake, Tomohide; Saijo, Masaaki; Motoki, Yuji

doi:10.3358/shokueishi.54.379

Cited by 7 publications

(5 citation statements)

References 9 publications

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“…Extraction and Quanti cation of L-DOPA Seeds of M. pruriens collected were grinded into ne powder and L-dopa content was determined and quanti ed by reversed phase highperformance liquid chromatography (RP-HPLC) method described by Hasegawa et al (2011). Dried sample of powdered seeds (100mg) was weighed and ultrasonically extracted for 15 minutes using 5ml of acetonitrile/water/ formic acid (50:50:1).…”

Section: Methodsmentioning

confidence: 99%

Genetic variability and divergence studies on seed traits and L-dopa content of Mucuna pruriens (L.) DC. accessions

Patel

Azeez

Krishnamurthy

2023

Preprint

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Mucuna pruriens (L.) DC. belongs to the Fabaceae family and is widely distributed across the tropical and subtropical regions of the world. Seeds of Mucuna are the industrially important source of natural L-dopa. The present study is intended to screen out the superior Mucuna germplasm which can be utilized for commercial cultivation. A total of 59 accessions of M. pruriens (L.) DC. were collected from various geographical locations across India and evaluated for seed-associated agronomic traits and L- dopa content in the seed. Morphological observations of seed samples revealed a wide variation in seed color pattern including complete white, black and striped/mottled. Quantitative traits such as 100 seed weight (90.30-150.59gm), seed length (5-19.3 mm), width (2.66-13.00 mm), thickness (0.90–8.67 cm), length width ratio (1.17–2.26), length thickness ratio (1.50–7.40), width thickness ratio (0.89-6.00) seed geometric diameter (2.34–12.60 mm), arithmetic mean diameter (2.88–13.33 mm), seed sphericity (41.89–78.75), seed surface area (17.19-498.51 mm2) and L-dopa content (1.79–6.05%) were evaluated in order to compare the features of the accessions collected from different places. The inter-association between morphological observations and L-dopa content was measured using principal component analysis and Ward’s hierarchical clustering, as these are of vital importance in selecting the best accession of velvet bean for breeding programs.

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Section: Methodsmentioning

confidence: 99%

Genetic variability and divergence studies on seed traits and L-dopa content of Mucuna pruriens (L.) DC. accessions

Patel

Azeez

Krishnamurthy

2023

Preprint

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“…For example, Mucuna pruriens, which is also a legume, is characterized as a major source of L-DOPA. In a study of dietary supplements containing M. pruriens, DOPA was enantiomerically analyzed; in all samples (14 samples), only L-DOPA was detected and in no sample was D-DOPA identified [37]. This suggests that in Vicia faba L. something similar could occur, but this still needs to be tested.…”

Section: Bioactive Compounds Determined By Hplc: Concentration Of L-d...mentioning

confidence: 97%

Content and Yield of L-DOPA and Bioactive Compounds of Broad Bean Plants: Antioxidant and Anti-Inflammatory Activity In Vitro

Fuentes-Herrera,

Herrera-Cabrera,

Martínez-Ayala

et al. 2023

Plants

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The broad bean plant contains L-DOPA, a compound that is essential for patients with Parkinson’s disease. However, little has been reported on other broad bean compounds that have beneficial effects on health. The objective was to evaluate plants of four Mexican broad bean varieties to determine the content and yield of total phenolic compounds (TPC), total flavonoids (TF), and L-DOPA, as well as to analyze the flavonoid profile and antioxidant (AA) and anti-inflammatory (AANTI) activity in vitro. Broad bean seeds were sown in the field and plants were harvested 20 days after emergence. The analyses were performed with visible UV spectrophotometry and HPLC. The variety José María produced the highest yield of TPC (9.30 g m−2), TF (8.08 g m−2), and L-DOPA (5.64 g m−2) per unit of area. The highest yields per plant were obtained with the Rojita variety: TPC (0.25 g plant−1), TF (0.21 g plant−1), and L-DOPA (0.17 g plant−1). This variety also had the highest antioxidant (IC50 = 87.68 µg mL−1) and anti-inflammatory (IC50 = 74.40 mg mL−1) activity, which was attributed to the L-DOPA compounds and to rutin and isoorientins, respectively. The flavonoid profile revealed the presence of rutin and isoorientins, which had not been previously detected in the broad bean plant.

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“…The dried plants were ground to a fine powder with a granite mortar and pestle. The plants were extracted by adapting the method of Hasegawa et al (2011). Ten milligrams of dried sample was mixed with 500 µl of acetonitrile/ water/formic acid (50 : 50 : 1).…”

Section: Extraction Of L-dopa From In Vitro Cultivated Plantsmentioning

confidence: 99%

Productivity of L-DOPA in in vitro shoots of Mucuna pruriens var. utilis enhanced by gamma radiation

Detmontree¹,

Marsud²,

Subhadhcha³

et al. 2020

J Appl Pharm Sci

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Enantiomeric Determination of DOPA in Dietary Supplements Containing <i>Mucuna pruriens</i> by Liquid Chromatography/Mass Spectrometry

Cited by 7 publications

References 9 publications

Genetic variability and divergence studies on seed traits and L-dopa content of Mucuna pruriens (L.) DC. accessions

Genetic variability and divergence studies on seed traits and L-dopa content of Mucuna pruriens (L.) DC. accessions

Content and Yield of L-DOPA and Bioactive Compounds of Broad Bean Plants: Antioxidant and Anti-Inflammatory Activity In Vitro

Productivity of L-DOPA in in vitro shoots of Mucuna pruriens var. utilis enhanced by gamma radiation

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