Enantiomeric methadone quantitation on real post-mortem dried matrix spots samples: Comparison of liquid chromatography and supercritical fluid chromatography coupled to mass spectrometry

Mueller, F.; Losacco, Gioacchino Luca; Nicoli, Raul; Guillarme, Davy; Thomas, Aurélien; Grata, Elia

doi:10.1016/j.jchromb.2021.122755

Cited by 13 publications

(6 citation statements)

References 38 publications

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“…The method published by Mueller et al 11 was used as a starting point for method development. As the choice of column has a critical impact on separation in chiral analysis using SFC, 16 the Chiralpak IH‐3 column as presented in the method by Mueller et al was chosen for method development.…”

Section: Resultsmentioning

confidence: 99%

“…9 Several chiral LC-MSMS methods for the separation of R/Smethadone and its inactive metabolite 2-ethylidine-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) have been presented. [10][11][12][13][14][15] Plasma, saliva, post-mortem whole blood and various tissues are matrices used in these published methods, and all of them report analytical runtimes of 12 minutes or more.…”

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confidence: 99%

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Quantitative determination of R/S‐methadone in human serum using ultra‐high performance supercritical fluid chromatography‐tandem mass spectrometry: A method for routine use

Hansen,

Havnen,

Andreassen

et al. 2024

Drug Testing and Analysis

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Methadone has two enantiomers, which exhibit differences in pharmacological effects, with R‐methadone being the active and S‐methadone the inactive enantiomer. A robust, simple and rapid method for chiral separation of the two enantiomers in serum samples using ultra‐high performance supercritical fluid chromatography‐tandem mass spectrometry (UHPSFC‐MSMS) has been developed and validated. Enantiomeric separation was achieved using a Chiralpak IH‐3 column with a mobile phase consisting of CO2 and 30mM ammonium acetate in methanol/water (98/2, v/v). Runtime was 4 minutes. Sample preparation was semi‐automated using a Hamilton ML Star robot with protein precipitation, and phospholipid removal was carried out using a Waters OSTRO™ 96‐well plate. The calibration range was 50.0–1,500 nM for each enantiomer. The between‐assay relative standard deviations were in the range of 1.2–3.6%. Matrix effects ranged from 99% to 115% corrected with internal standard. The method has been implemented in our laboratory and has proven to be a robust and reliable method for determining the ratio of R/S‐methadone in authentic patient samples.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Quantitative determination of R/S‐methadone in human serum using ultra‐high performance supercritical fluid chromatography‐tandem mass spectrometry: A method for routine use

Hansen,

Havnen,

Andreassen

et al. 2024

Drug Testing and Analysis

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“…Table 1 summarizes the details based on the use of LC techniques utilized for BUP and MTD measurement. LC can be coupled with ultraviolet (UV) [40][41][42][43]54], photodiode array detector (PAD) [40], fluorescence (FL) and electrochemical (EC) [29,38] MS detection [7,13,15,18,[23][24][25][26][27][28]49,50,52,56,[60][61][62]64,65,[68][69][70][71][72][73]88]. Reversed-phase columns (C 18 or C 8 columns) with spherical sorbent particles are popular for the determination of BUP, MTD and their metabolites.…”

Section: Liquid Chromatography Hyphenated Techniquesmentioning

confidence: 99%

“…MTD is a synthetic opioid, which is available as a racemic mixture, with R-methadone being more potent than S-methadone [15]. The use of MTD may result in overdose because it has no ceiling effect.…”

Section: Introductionmentioning

confidence: 99%

“…Different biological specimens including urine [7,, plasma [13,20,21,35,38,40,[42][43][44][45][46][47][48][49][50][51][52][53][54][55], serum [30,[56][57][58][59], blood [15,18,22,49,[60][61][62][63], nails [64], oral fluid [17,31,55,[65][66][67][68][69], hair [47,[70][71][72], tissue [73,74] and exhaled breath condensate [75,76] have been used for the measurement of BUP, MTD and their metabo...…”

Section: Introductionmentioning

confidence: 99%

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Analytical Approaches for the Determination of Buprenorphine, Methadone and Their Metabolites in Biological Matrices

Shan¹,

Cao²,

Yang³

2022

Molecules

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The abuse of buprenorphine and methadone has grown into a rising worldwide issue. After their consumption, buprenorphine, methadone and their metabolites can be found in the human organism. Due to the difficulty in the assessment of these compounds by routine drug screening, the importance of developing highly sensitive analytical approaches is undeniable. Liquid chromatography tandem mass spectrometry is the preferable technique for the determination of buprenorphine, methadone and their metabolites in biological matrices including urine, plasma, nails or oral fluids. This research aims to review a critical discussion of the latest trends for the monitoring of buprenorphine, methadone and their metabolites in various biological specimens.

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Supercritical Fluid Chromatography in Bioanalysis–A Review

Toribio,

Martín,

Bernal

2024

J of Separation Science

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In the last decade, the instrumentation improvements in supercritical fluid chromatography (SFC) and the hyphenation to mass spectrometry (MS), have increased the SFC acceptance between scientists, becoming today a valuable tool in analytical chemistry. The unique selectivity, short analysis times, low consumption of organic solvents, and the greener mobile phase, have contributed to expanding its applicability which has led to an increase in the number of publications especially in the bioanalysis area. This work reviews the advantages and main applications of SFC in bioanalysis during the last 5 years. Fundamental aspects concerning mobile phase composition, stationary phase, hyphenation to MS as well as matrix effect have been discussed. Finally, the most relevant applications have been summarized.

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Enantiomeric methadone quantitation on real post-mortem dried matrix spots samples: Comparison of liquid chromatography and supercritical fluid chromatography coupled to mass spectrometry

Cited by 13 publications

References 38 publications

Quantitative determination of R/S‐methadone in human serum using ultra‐high performance supercritical fluid chromatography‐tandem mass spectrometry: A method for routine use

Quantitative determination of R/S‐methadone in human serum using ultra‐high performance supercritical fluid chromatography‐tandem mass spectrometry: A method for routine use

Analytical Approaches for the Determination of Buprenorphine, Methadone and Their Metabolites in Biological Matrices

Supercritical Fluid Chromatography in Bioanalysis–A Review

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