Enantiomeric separation of norgestrel by reversed phase high-performance liquid chromatography using eluents containing hydroxypropyl-beta-cyclodextrin in stereoselective skin permeation study

Advances in the analysis of steroid hormone drugs in pharmaceuticals and environmental samples (2004–2010)

“…For example, ion-pairing reagent (octanesulfonic acid) was added to the eluent for the determination of the quaternary ammonium derivative pancuronium bromide [91] and hydroxypropyl-␤-cyclodextrin for the separation of norgestrel enantiomers [92]. A special branch of HPLC, micellar liquid chromatography where micelle forming agents such as in this case cetyl trimethyl ammonium bromide are added to the eluent was applied to the determination of betamethasone and dexamethasone in tablets and in a cocktail containing both drugs [93].…”

Enantiomeric separation of 2-arylpropionic acid nonsteroidal anti-inflammatory drugs and β-blockers by RP-HPLC using an amylose chiral stationary phase for the enantioselective skin permeation study

“…In vitro skin permeation studies of S-, R-and RS-PL hydrochloride across the excised rat dorsal skin were carried out according to the procedures described in our previous study. 19 The skin was mounted on the receptor compartment of a two-chambered Valia-Chien glass diffusion cell at 32 C with stirring at a constant rate of 500 rpm. The Valia-Chien cell was composed of a receptor compartment and a donor compartment, with a volume of 4 ml for each compartment, and an effective diffusion area of approximately 0.65 cm 2 .…”

Chromatographic and Computational Studies on the Chiral Recognition of Sulfated β-Cyclodextrin on Enantiomeric Separation of Milnacipran

“…The survey of literature revealed that there are chromatographic methods for enantiomeric separation of chiral drugs using β-cyclodextrin as chiral mobile phase additives [7][8][9][10][11][12][13][14]. But there are no reported methods for enantiomeric separation of milnacipran using chiral mobile phase additives.…”